Steroids p. 275 - 282 (1984)
Update date:2022-08-04
Topics:
Nordblom, Gerald D.
Counsell, Raymond E.
England, Barry G.
Antibody used in a steroid radioimmunoassay raised against a steroid hapten-carrier protein conjugate may recognize both the hapten and the chemical bridge to the protein.Use of the same bridge in the radioisotopic label may lead to higher affinity binding to the label than to the native steroid.Inhibition curves under these conditions are shallow and generally not acceptable for radioimmunoassay procedures.We have developed a radioimmunoassay for androstenedione that employs different bridges at the 11β position of the steroid for the protein conjugate and label.The resulting assay has greatly reduced bridgebinding, has an acceptable slope for the standard curve and is very specific as evidenced by low crossreactivies to other steroids.
View MoreBrightGene Bio-Medical Technology Co., Ltd.
website:https://en.bright-gene.com/
Contact:+86-512-62551801
Address:Building C25 - C31, No. 218 Xinghu Road, Suzhou Industrial Park, Suzhou, Jiangsu, China.
Daicel Chiral Technologies (China)CO.,LTD
Contact:021-5046-0086*8
Address:Part C, FL 5, the 16th Building, No. 69, XiYa Road, WaiGaoQiao Free Trade Zone, Shanghai, 200131, P.R.China
Hangzhou Eastbiopharm Co.,Ltd.
Contact:+86-571-88931780
Address:Hangzhou,China
Hangzhou Mole's Science & Technology Co.,Ltd.(expird)
Contact:+86-571-56880228
Address:15F Guodu development Building, NO.182 Zhaohui Road
Shandong LuZhou Amino Acid Co., Ltd
Contact:86-539-2218025
Address:yishui economic and technical development zone zhenxing south road
Doi:10.1016/S0957-4166(98)00155-4
(1998)Doi:10.1021/jm4017762
(2014)Doi:10.1002/ejoc.201400142
(2014)Doi:10.1039/c5cc00780a
(2015)Doi:10.1021/acs.joc.0c02667
(2021)Doi:10.1134/S1068162014030042
(2014)