Polymer-Supported Photosensitizers for Oxidative Organic Transformations in Flow and under Visible Light Irradiation

Article abstract of DOI:10.1021/acscatal.7b00888

A 2,1,3-benzothiadiazole (BTZ)-based vinyl cross-linker was synthesized and copolymerized with large excesses of styrene using free radical polymerization to deliver heterogeneous triplet photosensitizers in three distinct physical formats: gels, beads, and monoliths. These photosensitizers were employed for the production of singlet oxygen (¹O₂) and for the aerobic hydroxylation of arylboronic acids via superoxide radical anion (O₂^?-), whereby the materials demonstrated good chemical and light stability. BTZ-containing beads and monoliths were exploited as photosensitizers in a commercial flow reactor, and ¹O₂ production was also demonstrated using direct sunlight irradiation, with a conversion rate comparable to the rates achieved when a 420 nm LED module is used as the source of photons.

Full text of DOI:10.1021/acscatal.7b00888

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Article
Polymer-Supported Photosensitizers for Oxidative Organic
Transformations in Flow and Under Visible Light Irradiation
John M Tobin, Timothy J. D. McCabe, Andrew Prentice, Sarah Holzer, Gareth O.
Lloyd, Martin J. Paterson, Valeria Arrighi, Peter A.G. Cormack, and Filipe Vilela
ACS Catal., Just Accepted Manuscript • Publication Date (Web): 02 Jun 2017
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PolymerꢀSupported Photosensitizers for Oxidative
Organic Transformations in Flow and Under Visible
Light Irradiation
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John M. Tobin,† Timothy J. D. McCabe,‡ Andrew W. Prentice,† Sarah Holzer, † Gareth O.
Lloyd,† Martin J. Paterson,† Valeria Arrighi,† Peter A. G. Cormack*,‡ and Filipe Vilela*,†
†School of Engineering and Physical Sciences, HeriotꢀWatt University, Edinburgh, EH14 4AS,
Scotland, United Kingdom.
‡WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Thomas
Graham Building, 295 Cathedral Street, Glasgow, G1 1XL, Scotland, United Kingdom.
ABSTRACT. A 2,1,3ꢀbenzothiadiazole (BTZ)–based vinyl crosslinker was synthesized and
copolymerized with large excesses of styrene using free radical polymerization to deliver
heterogeneous triplet photosensitizers in three distinct physical formats: gels, beads and
monoliths. These photosensitizers were employed for the production of singlet oxygen (1O2) and
for the aerobic hydroxylation of aryl boronic acids via superoxide radical anion (O2˙ꢀ) whereby
the materials demonstrated good chemical and photo stability. BTZꢀcontaining beads and
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monoliths were exploited as photosensitizers in a commercial flow reactor, and O2 production
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was also demonstrated using direct sunlight irradiation, with a conversion rate comparable to the
rates achieved when using a 420 nm LED module as the source of photons.
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Keywords: flow chemistry, free-radical polymerization, heterogeneous photosensitizer, reactive
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oxygen species, singlet oxygen, visible light
INTRODUCTION
New advances in photocatalytic processes (including new photocatalysts) have been made in
recent years, not only in the production of energy dense materials, as shown in the field of Solar
Fuels (with extensive studies on achieving waterꢀsplitting and CO2 reduction), but also in organic
synthesis.1ꢀ3 These efforts represent a true advancement in realizing more sustainable,
economical and environmentally friendly strategies for a wideꢀrange of chemical processes.
One photocatalytic process that has been under scrutiny is the generation of singlet oxygen
(1O2) through wellꢀknown photosensitization reactions.4, 5 These reactions proceed under mild
conditions, requiring only a photosensitizer, a light source and molecular oxygen. Organic dyes
such as Rose Bengal, Eosin Blue and Methylene Blue, among others, are some of the most
commonly used photosensitizers in this process.6ꢀ11 When irradiated with light of an appropriate
wavelength, energy is absorbed by the photosensitizer which is excited electronically to the
triplet state through an intersystem crossing mechanism. The excited photosensitizer then
transfers this energy to the ground state triplet oxygen (3O2), resulting in the formation of O2.7
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The electrophilic and oxidizing properties of O2 have driven its use as an oxidant and as a
synthetic reagent in a wide range of applications, such as organic synthesis,12 water and
wastewater treatment13 and photodynamic therapy for the treatment of cancer.14, 15
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Another area of catalysis that has come to prominence recently is that of photoredox reactions
for organic synthesis.16 Along with the generation of 1O2, photooxidations use molecular oxygen
in air as a green reagent, resulting in highly desirable reaction conditions. However, the reactive
species for these reactions involve a superoxide radical anion (O2˙ꢀ), not the aforementioned 1O2.
Formation of O2˙ꢀ can proceed through a photoredox cycle in the presence of an amine base
acting as a sacrificial reductant, a photosensitizer capable of occupying the excited triplet state
and molecular oxygen.17
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While both reactive forms of molecular oxygen are generally wellꢀknown and have their uses
in a variety of applications, many of the photocatalytic materials used rely largely on transition
metal complexes or organic dyes, which can lead to several issues. For example, the use of
organometallic photosensitizers, while efficient light harvesters,17ꢀ21 tend to be expensive,
unsustainable and toxic. Some photosensitizers are also prone to photobleaching, i.e., the
degradation of the photosensitizer due to prolonged exposure to light and/or reactivity with 1O2.7
This leads to both a reduction in the efficiency and the lifetime of the photosensitizer. Lastly,
most of these materials are employed under homogeneous conditions, leading to a cumbersome
separation and recovery process of the photosensitizer from the reaction mixture, and ultimately
the desired products, which impedes the reusability of the photosensitizer.22 These disadvantages
demonstrate the need to develop metalꢀfree and photostable materials that can act as
heterogeneous photosensitizers in a variety of reactions and conditions.
In principle, the use of heterogeneous photosensitizers offers advantages in practical
applications when compared to their homogeneous counterparts because they are easy to isolate,
recover and recycle. Immobilization of commonly used homogeneous photosensitizers, such as
organic dyes23, 24 and transition metal complexes,25 in polymer matrices has been shown to
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provide a more photostable material. This is due to the polymer matrix acting as an efficient
oxygen diffusion barrier whilst inhibiting photobleaching of the photosensitizer.26 However, this
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immobilization technique may lead to a decrease in the quantum yield of O2 production and
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therefore its efficiency as a photosensitizer.7 Nevertheless, the added benefits of a heterogeneous
photosensitizer (ease of recovery, reusability and avoiding contamination of the product)
outweigh the lower efficiency of the photocatalytic material.
Recently, we reported the use of conjugated microporous polymers (CMPs) as heterogeneous
triplet photosensitizers. Through visible light irradiation, these 2,1,3ꢀbenzothiadiazole (BTZ) 27ꢀ29
or 4,4ꢀdifluoroꢀ4ꢀboraꢀ3a,4aꢀdiazaꢀsꢀindacene (BODIPY)ꢀrich30 CMPs have been shown to be
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efficient and reusable materials for the production of O2. Reports employing heterogeneous
photocatalysts for photoredox reactions have begun to emerge, primarily through the use of
CMPs.31 However, along with this work, CMP synthetic strategies largely involve Pd catalysts,
resulting in an expensive material that is likely to be contaminated with residual Pd. Residual
metal, even at ppb levels, is problematic as it may interfere with catalytic processes or lead to the
toxicity of the reaction medium due to leaching. To circumvent this issue, we have developed a
photoactive vinyl monomer, based upon the photoactive repeat unit used within the BTZꢀ
CMPs,27ꢀ29 which can be incorporated with ease into polymer matrices via free radical
copolymerization. The BTZꢀbased monomer contains two styrene units, which results in an
electronic “pushꢀpull” system, in which the BTZ unit acts as the strong electron acceptor and the
styrene as a weak electron donor. Similarly to the CMPs, we expected that this system would
allow the monomer, and polymers derived from the monomer, to act as photosensitizers for the
production of 1O2.
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One of the most widely available monomers for the production of polymers is styrene, with
over 25 million tons produced globally every year.32 It is a highly versatile monomer used to
prepare a variety of polymer architectures including linear, branched and crosslinked polymers.
Various formats of polystyrene have been described, such as foams, gels, beads and monolithic
structures.33
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Herein, the synthesis and characterization of a photosensitizing crosslinking monomer and its
incorporation into polystyrene structures through free radical copolymerization is presented. A
BTZꢀbased crosslinker was designed with two styrene units (Scheme 1) allowing for its
exploitation as a coꢀmonomer together with styrene and/or divinylbenzene (DVB) in traditional,
as well as more specialized, free radical polymerization techniques. Using 3ꢀ5 mol % only of the
BTZꢀbased crosslinker, three insoluble forms of photoactive polystyrene were synthesized: gels,
micrometerꢀsized polymer beads and monoliths. Computational models of the monomer and
polymer repeat unit were investigated to demonstrate their ability to occupy the triplet excited
state and thereby act as triplet photosensitizers. To validate these models, both the monomer and
corresponding polymers were evaluated for the production of 1O2 in chloroform under both batch
and flow conditions under visible light irradiation (420 nm). Furthermore, the same materials
were screened as triplet photosensitizers in aerobic hydroxylation of a series of aryl boronic acids
via the formation O2˙ꢀ. These materials have been presented as a proof of concept where both
computational and catalytic experiments verify them as efficient and stable triplet
photosensitizers.
EXPERIMENTAL
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General polymer synthesis and photocatalytic procedures will be described henceforth. All
other information pertaining to specific experimental methods not presented here can be found in
the ESI.
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Synthesis of 4,7-distyrene-2,1,3-benzothiadiazole (St-BTZ). To a dry, 2ꢀnecked flask equipped
with a magnetic stirring bar and a reflux condenser was added a mixture of 4,7ꢀdibromoꢀ2,1,3ꢀ
benzothiadiazole (147 mg, 0.5 mmol), 4ꢀvinylphenylboronic acid (148 mg, 1 mmol), and
Pd(PPh3)4 (17 mg, 0.015 mmol). The flask was connected to a Schlenk line, evacuated and backꢀ
filled three times with N2. In the flask whilst under N2, dry THF (40 mL) was added to dissolve
the solid reagents. An aqueous solution (5 mL) was then prepared with K2CO3 (140 mg, 1
mmol), degassed for approximately 10 minutes with N2 and added to the organic solution. The
mixture
Scheme 1. Reaction schemes for the synthesis of the monomer (St-BTZ) and gelꢀtype polymers
(Gel (M%)).
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was heated to 70 °C and left overnight, stirring under a N2 atmosphere. The reaction mixture was
allowed to cool to room temperature before adding approximately 40 mL of water and
transferring to a separating funnel with 50 mL of dichloromethane. The organic layer was
washed three times with deionized water and the collected fractions were dried over Na2SO4.
The crude mixture was filtered and purified via silica gel column chromatography with
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hexane:CH2Cl2 (4:6) as eluent, yielding a bright yellow colored solid (96 mg, 56%). H NMR
(300 MHz, CDCl3): δppm = 5.36 (d, 1 H), 5.89 (d, 1 H), 6.85 (m, 1 H), 7.63 (d, 2 H), 7.83 (s, 1 H),
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8.00 (d, 2 H); C NMR (100 MHz, CDCl3): δppm = 114.5, 126.5, 127.6, 129.4, 133.0, 136.4,
136.8, 137.7, 154.1.
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General synthesis of Styrene-St-BTZ copolymers via free radical polymerization (Gel
(M%)). Styrene (1 g, 9.6 mmol) was added to a sample vial with a magnetic stirring bar and
dissolved in 5 mL of degassed toluene. St-BTZ (2, 3 or 5 mol %) and AIBN (16 mg, 0.096
mmol) were added to the solution, allowed to dissolve and degassed with N2 for 10 minutes. The
vial was sealed, heated to 60 °C and left for 16 h to polymerize while stirring. The solution
began as a bright, yellow liquid and changed according to the percentage of St-BTZ added. The
reaction was allowed to cool to room temperature and the product was removed from the vial and
worked up as follows: (i) Precipitation of product was performed in 100 mL of MeOH, for
soluble copolymers; (ii) Soxhlet extraction was performed for insoluble (crosslinked)
copolymers with THF and methanol. All copolymers were dried in vacuo. It is noted that only
those polymers containing 3 or more mol % of the St-BTZ crosslinker produced an insoluble
gel. At concentrations lower than 3 mol % a soluble polymer was formed. Product recovery: Gel
(2%) 367 mg, 37 %; Gel (3%) 240 mg, 24 %; Gel (5%) 465 mg, 47 %.
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Synthesis of Styrene-DVB-St-BTZ copolymer via precipitation polymerization (Bead-
BTZ). DVBꢀ55 (221 mg, 1.7 mmol), styrene (666 mg, 6.4 mmol), St-BTZ (115 mg, 0.3 mmol)
(2% w/v total monomer in feed, relative to solvent) and AIBN (41 mg, 0.2 mmol) (2 mol %
relative to polymerizable double bonds in monomers) were added to acetonitrile (50 mL) in a
Nalgene® bottle (250 mL). Note that the monomer mole ratio employed for this synthesis was
76:20:4 (styrene:DVBꢀ55:St-BTZ), providing a good comparative analogue to the gels. The
bottle contents were ultrasonicated for 15 minutes. After ultrasonication, N2 gas was bubbled
through the reaction mixture for 15 minutes in an iceꢀbath. After degassing, the Nalgene® bottle
was sealed under N2 and placed on a low profile roller (Stovall Life Sciences Inc., North
Carolina, U.S.A) contained within an incubator (Stuart Scientific, Stone, UK) and rotated slowly
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about its long axis. The temperature inside the incubator was ramped from ambient temperature
to 60 °C over a period of around two hours. The polymerization was allowed to proceed for a
further 46 hours to give a milky suspension of polymer particles. The resulting particles were
visualized via optical microscopy prior to filtration. The product was filtered by vacuum on a
0.45 µm nylon membrane filter. The particles were then washed with solvent to remove any
unreacted monomer or initiator (~100 mL of acetonitrile followed by ~100 mL of toluene,
methanol and finally acetone) before being dried overnight in vacuo (60 mbar, 40 °C). Product
recovery: 120 mg, 12 %.
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Synthesis of Styrene-DVB-St-BTZ copolymer via high internal phase emulsion
polymerization (pHIPE-BTZ). Styrene (0.213 g, 2.04 mmol), DVBꢀ80 (40 mg, 0.29 mmol), St-
BTZ (25 mg, 0.07 mmol) and Span 80 (0.054 mL) were added to a sample vial and potassium
persulfate (18 mg, 0.2 % wt. of water) and sodium chloride (90 mg, 1 % wt. of water) were
dissolved in water (9 mL) in a separate vial. Note that the monomer mole ratio was 85:12:3
(styrene:DVB:St-BTZ), providing a good comparative analogue to the gels. The organic phase
was placed in an electric mixer (Polytron PT 1200 E) and the aqueous solution was added
slowly. The addition of the aqueous solution was stopped when the emulsion could no longer be
mixed properly due to thickening. Remaining liquid was decanted and part of the emulsion was
delivered into a transparent glass column (7 mm inner diameter) equipped with porous frit on
both ends via syringe. The column was sealed and placed in a 60 °C oven and left for 24 h. The
column containing the synthesized polyHIPE was flushed with 100 mL of THF, CH2Cl2 and
chloroform. The bright yellow monolith (35 mm length x 7 mm diameter) was left in the glass
column to be used for flow experiments.
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Computational modelling of St-BTZ excited states. Geometry optimization was performed
with density functional theory (DFT) using both the CAMꢀB3LYP and MN12ꢀL functionals,
while excited electronic states were computed with timeꢀdependent DFT, utilizing the temporal
adiabatic approximation with the same functionals. The 6ꢀ311G(d,p) basis set was used for all
computations. Analytical Hessian computation was used to confirm optimized stationary
geometries as minima. We note only negligible difference between functionals and so discuss the
CAMꢀB3LYP results below (full results for both are given in the supporting information). All
computations were performed using Gaussian09.34
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General procedure for photocatalytic reactions under batch conditions. To a vial
containing the required solution (5ꢀ10 mL), 5ꢀ10 mg of photosensitizing material was added. The
mixture was left to stir, whilst open to air, for 10 minutes to allow for proper dissolution or
swelling and to aerate the solution. The vial was then placed 7 cm from a 420 nm LED lamp and
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the lamp was switched on. The reaction was monitored via TLC and/or H NMR spectroscopy
until full consumption of the starting materials was noted, at which time the reaction was stopped
and analyzed.
General procedure for photocatalytic reactions in flow employing Bead-BTZ. To a flask
containing the required solution (10ꢀ15 mL), 5 mg of Bead-BTZ was added. The suspension was
first sonicated for 2 minutes to ensure an even dispersion of particles. The mixture was then left
to stir, whilst open to air, for 10 minutes to aerate the solution. The dispersion was then pumped
through the photochemical reactor (Figure S2) at a flow rate of 1 mL.minꢀ1. Concurrently, air
was pumped through a second pump at the same flow rate, mixing with the dispersion at a Tꢀ
junction before entering the photochemical reactor equipped with a 420 nm LED lamp. The
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dispersion was cycled for the required time and monitored via TLC and/or H NMR
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spectroscopy. Upon consumption of the starting materials, the reaction was stopped and
evaluated further.
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General procedure for photocatalytic reactions in flow employing pHIPE-BTZ. The
pHIPE-BTZ monolith, fitted to a transparent glass column, was allowed to swell in the required
solvent for 16 hours. The solution containing the starting materials (10 mL) was prepared and
aerated for 10 minutes open to air. The column was fitted to a light source (Figure S3) and the
solution was pumped through the column at 0.5ꢀ1.0 mL.minꢀ1. Concurrently, oxygen was
pumped through a second pump at the same flow fate, mixing with the solution at a Tꢀjunction
before entering the column. The operating pressure was kept at 1.3 bar to ensure a steady stream
of both the solution and air. The 10 mL solution was pumped through the column and monitored
via TLC and/or 1H NMR spectroscopy. Upon consumption of the starting materials, the reaction
was stopped and evaluated.
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RESULTS AND DISCUSSION
Monomer properties. The UVꢀVis absorption spectra for St-BTZ presented as a broad
absorption peak in the range 350 to 450 nm with a maximum absorbance at 400 nm (Figure S13).
This falls within the visible spectrum and is comparable to the absorbance characteristics which
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we have reported for other BTZꢀbased photosensitizers.27ꢀ29 FTꢀIR, H NMR and 13C NMR
spectroscopic analysis was fully consistent with the structure of St-BTZ (see ESI). An Xꢀray
crystal structure and the corresponding analysis data can also be found in the ESI.
By virtue of its polymerizable vinyl groups, St-BTZ can be copolymerized with styrene via
free radical polymerization. Similarly to DVB, St-BTZ can act as a branching node and
consequently as a crosslinker. Employing the crosslinker at an appropriate concentration in a
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copolymerization can result in an insoluble, crosslinked polymer that can then be employed as a
heterogeneous photosensitizer.
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Synthesis and properties of Gel (M%). One of the key aims of the work was to minimize the
amount of photoactive St-BTZ required whilst ensuring the formation of an insoluble polymer
with the desired photosensitizing properties. The selection of styrene as coꢀmonomer as the basis
for the heterogeneous photosensitizer was influenced by the stability of polystyrene in the
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presence of O2 and the fact that the photoactive monomer also contains styrene units, which
leads to a more compatible copolymerization between the coꢀmonomers.
The formation of these polymers (Gel (3%) and Gel (5%)) suggests that there was sufficient
crosslinking of the polymer chains to create an insoluble material. The bright yellow color,
similar to that of St-BTZ, is also indicative of the integration of the photoactive crosslinker into
the polymer network. Most of the solvent used in these reactions was sorbed by the polymers,
resulting in bright yellow, swollen gels (Figure 1). A swelling test was performed on dried Gel
(3%), demonstrating an increase of up to seven times its original volume when added to
chloroform (Figure S1).
UVꢀVis absorption spectra of the materials showed a broad absorption band (350ꢀ450 nm)
with a maximum at 400 nm (Figure S13). FTꢀIR spectroscopic measurements showed peaks
corresponding to St-BTZ residues (825 and 887 cmꢀ1), which were not observed in a polystyrene
standard (Figure S12). Note that these signals were relatively weak due to the high ratio of
styrene to St-BTZ within the polymer.
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Figure 1. Inverted reaction vials showing Gel (3%) (left) and Gel (5%) (right) as insoluble gels
swollen in chloroform.
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Solid state C NMR CPꢀMAS spectroscopy was also employed to characterize Gel (3%).
Whilst aromatic carbons are present primarily, at 128 ppm (CꢀC) and 146 ppm (CꢀN), signals in
the alkyl region are also identified at 40ꢀ45 ppm and correspond to the saturated aliphatic
backbone of the copolymer (Figure S18). These observations suggest that St-BTZ was
incorporated successfully into the polystyrene chains via wellꢀestablished, metalꢀfree, free
radical polymerization and that the polymer has the potential to be used a polystyreneꢀbased
heterogeneous photosensitizer.
Heterogeneous phase polymerizations (including emulsion, dispersion, precipitation and
suspension polymerization)33 using the same free radical initiator allow us to demonstrate the
versatility of the photoactive crosslinker. This versatility arises from the ability of St-BTZ to be
implemented in a variety of free radical polymerization techniques whilst yielding polymers
which retain the photoactive properties of the monomer. For the purposes of this study,
precipitation polymerization and high internal phase emulsion (HIPE) polymerization were
employed, which allows for the synthesis of the polymers in different physical formats derived
from the same monomer set: micronꢀsized beads from precipitation polymerizations and
monolithic structures from HIPE polymerizations.
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Precipitation polymerization (Bead-BTZ). As seen in Scheme S2, precipitation
polymerization utilizing a free radical initiator was used to synthesize particles in the form of
micronꢀsized beads. In order to ensure the production of an insoluble polymer, divinylbenzeneꢀ
55 (DVBꢀ55) was added as a nonꢀphotoactive crosslinker. The incorporation of DVB as a
crosslinker also adds rigidity to the structure and good mechanical robustness to the beads. In
parallel with the photoactive polymer synthesis, a styreneꢀDVB copolymer (Bead) was
synthesized without any photoactive component for comparison.
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UVꢀVis spectroscopy showed a maximum absorption at ~420 nm for Bead-BTZ, which is
comparable to the absorbance wavelength of the previously described materials (Figure S15).
Bead did not display any absorption in the visible region and produced a white product
compared to the bright yellow Bead-BTZ material. An FTꢀIR spectrum of Bead-BTZ showed
the two distinct peaks characteristic of St-BTZ (887 and 825 cmꢀ1). These peaks were not
observed for Bead (Figure S14). Figures 2a and 2b show both bead types together with the
corresponding SEM images. These images show the polymer microspheres very clearly, with a
bead size range of approximately 1ꢀ2 µm for both materials. While Bead resulted in betterꢀ
defined particles, Bead-BTZ showed some signs of aggregation and agglomeration. Based on
these observations, we concluded that the St-BTZ crosslinker was integrated successfully into
the polystyrene beads.
High Internal Phase Emulsion Polymerization (pHIPE-BTZ). The synthesis of pHIPE-
BTZ was carried out through the free radical polymerization of a high internal phase emulsion
(Scheme S3). A UVꢀVis spectrum showed a maximum absorption at ~420 nm for pHIPE-BTZ,
which is comparable to the absorption wavelengths of the other synthesized polymers (Figure
S15). An FTꢀIR spectrum showed only weak bands attributable to St-BTZ (887 and 825 cmꢀ1)
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(Figure S14). Figure 2c shows pHIPE-BTZ in the column and the corresponding SEM image.
This image demonstrates the high degree of porosity typical of polyHIPEs. Again, DVB was a
crucial component to stabilize the structure as attempts with only St-BTZ (3ꢀ5 mol %) as the
crosslinker resulted in amorphous, gelꢀlike polymers.
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Figure 2. Synthesized copolymers and the corresponding SEM images: a) Bead containing no
St-BTZ; b) Bead-BTZ with a yellow color indicating incorporation of St-BTZ; c) pHIPE-BTZ
contained within a column as a stationary material for flow applications.
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Given the variety of freeꢀradical polymerization techniques used, the synthesized monomer
showed exceptional versatility and stability. By incorporating very limited amounts of the
monomer into a polymer matrix, we were able to dilute the amount of photoactive component
when compared to more common materials, as previously described. To better understand these
materials as potential triplet photosensitizers, computational excited state data were gathered.
St-BTZ excited state computational modelling. Computations have been performed to
investigate the lowest singlet and triplet state geometries, and a characterization of vertical
electronic excited states, for the St-BTZ monomer and in the absence of vinyl groups as it would
appear in the photoactive polymers. For the systems investigated both C2 and Cs ground state
singlet conformers are found (differing in relative sign of dihedral twist for the benzene rings).
Each isomer is essentially isoenergetic and results below are for the C2 matching the crystal
structure (further comparison in ESI). We note generally very good agreement for the S0
geometry with experiment. For both systems the lowest singlet and triplet states are of the same
character, being 94% represented by the HOMOꢀLUMO orbital transition (Table S1 and S2).
This corresponds to charge transfer through the πꢀsystem from the styreneꢀbenzene/isopropylꢀ
benzene groups to the BTZ group as shown in Figure 3 below for the monomer and polymer
systems. Finally in Table S3 we give the singletꢀtriplet energy gap computed at the optimized
triplet state geometry for both systems. There is around a 0.8 eV triplet stabilization from the
vertical geometry for both systems.
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Computational verification of the synthesized materials demonstrated their potential to occupy
the triplet excited state. These results showed promise and prompted investigations into their
catalytic capabilities. For the purposes of this study, we explored their ability to act as triplet
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photosensitizers in both the production of O2 and O2˙ꢀ. Furthermore, due to the photoactive
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component being dramatically reduced within the polymeric materials, it was of great interest to
understand if this had any significant effect on their ability to act as photosensitizers.
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Figure 3. NTO particleꢀhole representation of a) St-BTZ monomer and b) polymer repeat unit
T1 state, computed with TDꢀCAMꢀB3LYP/6ꢀ311G(d,p).
Homogeneous photosensitization at 420 nm with St-BTZ. The St-BTZ crosslinker was first
examined as a homogeneous triplet photosensitizer in the production of 1O2. Scheme 2 shows the
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Alderꢀene reaction, which only proceeds in the presence of O2.36 The production of O2 was
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monitored by observing the conversion of αꢀterpinene into ascaridole via H NMR spectroscopy
(Figure S20). Full conversion to ascaridole was observed after 90 minutes of irradiation.
However, a sudden decrease in the rate of conversion of αꢀterpinene to ascaridole was observed
after 45 minutes, causing near full conversion to take upwards of 90 minutes.
Scheme 2. Synthesis of ascaridole from αꢀterpinene via the photosensitization of singlet oxygen
under visible light irradiation.
O
CHCl3, Photosensitizer
O
Air,
λ @ 420 nm
α-terpinene
Ascaridole
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Samples taken at 15ꢀminute time intervals revealed a dramatic color change (Figure S21),
prompting further investigations to determine any potential photoꢀdegradation or oxidative effect
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on St-BTZ. H NMR spectroscopic analysis for each sample confirmed the degradation of the
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photosensitizer, where a clear decrease in the characteristic peaks for the monomer was observed
(Figure S20).
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Unsurprisingly, interactions between O2 and vinylarenes can result in the production of
monoadducts and endoperoxides.37 The reaction potential of the vinyl groups may also have
increased due to the electron withdrawing effects of the BTZ component within the monomer.
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Along with the known reactivity between vinylarenes and O2, these observations demonstrate
the instability of St-BTZ under highly oxidative conditions. Degradation issues aside, we were
able to demonstrate the ability of St-BTZ to act as a triplet photosensitizer in the production of
1O2 under visible light irradiation. However, we have shown in the past that when incorporating
BTZꢀbased building blocks into CMPs, the stability of the resulting CMP upon production of 1O2
is increased when compared to the monomeric version of BTZ.27ꢀ29 Therefore, and knowing that
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polystyrene is largely unaffected by O2,35 we postulated that incorporating St-BTZ into a
polystyrene matrix would mitigate the photoꢀdegradation or oxidative effect on the polymer.
Heterogeneous photosensitization at 420 nm with Gel (M%). A procedure similar to that
described above for St-BTZ (Scheme 2) was employed with a reaction time of 60 minutes. Three
control experiments were performed first, as follows: (i) in the absence of any photosensitizing
material; (ii) using a polystyrene standard as the photosensitizer; (iii) performing the experiment
in the dark and in the presence of Gel (3%). Each of these controls showed no conversion of αꢀ
terpinene to ascaridole, and therefore that 1O2 was not produced under these conditions.
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As seen in Figure 4, both Gel (3%) and Gel (5%) showed comparable conversion rates, with
close to full conversion achievable after 60 minutes of irradiation. Interestingly, compared to
these heterogeneous photosensitization experiments, both homogeneous analogues, St-BTZ and
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Gel (2%), gave significantly reduced conversion rates. The reduction in the rate of O2
production with Gel (2%) can be explained by a decrease in the level of the photoactive
monomer within the material. As the photoactive St-BTZ content decreases, there are fewer
accessible sites for an energy transfer to 3O2, resulting in reduced production of 1O2. The reduced
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rate of O2 production with St-BTZ has been explored as explained above. H NMR spectra
corresponding to each reaction can be found in the ESI.
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St-BTZ
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Gel (3%)
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0
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Time (min)
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Figure 4. Conversion rates of αꢀterpinene to ascaridole using O2 produced from the irradiation
of St-BTZ, Gel (2%), Gel (3%), and Gel (5%) with a 420 nm LED lamp.
Based on these results, it is clear that the insoluble photoactive gels performed more efficiently
than their soluble counterparts. While Gel (5%) performed slightly better than Gel (3%), it also
required a greater amount of the photoactive monomer. The gels also displayed greater stability
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than St-BTZ; no appreciable color change was observed over the reaction period. Most notably,
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these results demonstrate the generation of O2 with a heterogeneous photosensitizer where the
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main component is polystyrene (95ꢀ97%). This is advantageous and costꢀeffective since it
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reduces the amount of St-BTZ required in the copolymer to produce O2 when compared to
other heterogeneous photosensitizers where each repeat unit consist of a BTZ monomer.7, 27ꢀ29
Moreover, selfꢀquenching of the desired triplet state between photocatalytic segments of the
polymer can occur if the concentration of the photoactive component is too great. This can
greatly hinder the triplet state lifetime, and therefore diminish the effectiveness of the material as
a photosensitizer. By reducing the concentration of St-BTZ the selfꢀquenching effect is
mitigated greatly without compromising the ability of the polymers to act as photocatalysts.8
These concepts are crucial when investigating routes to both reduce the cost and increase the
sustainability and effectiveness of photoactive materials.
Application of Bead-BTZ and pHIPE-BTZ in continuous flow. Continuous flow chemistry,
as an alternative to standard batch synthesis, offers a flexible route to photochemical reactions,
particularly when approaching industrial scales.38 Reactions using this method allow for
streamlined and continuous production of materials along with the potential for workꢀup and
analysis integration.39 This is critically important within industrial processes as it has the
potential to both reduce costs and increase productivity. This is especially true for photochemical
transformations as the irradiation of light to a solution is more efficient due to the shorter path
lengths when compared to traditional batch processes. On larger industrial scales, photon
penetration depth and excessive irradiation can be problematic under traditional batch
conditions.40 Continuous flow addresses these issues through higher surfaceꢀtoꢀvolume ratios and
more efficient heat transfer. This allows for investigations into new synthetic pathways and
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reaction methodologies.41 Photochemical reactions have been demonstrated widely using
continuous flow methods, even under heterogeneous conditions.29, 41
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continuous flow chemistry was impractical. When attempted in flow, the gel particles aggregated
and coagulated within the tubing. For this reason, Bead-BTZ and pHIPE-BTZ were designed
specifically for two distinct continuous flow applications: (i) The polymer beads present a
spherical shape and a low mean particle diameter (1ꢀ2 µm), which allows for facile dispersion
throughout a solvent and low susceptibility to aggregation. These properties allow a dispersion of
beads within the reaction mixture to flow through a photochemical reactor with greater ease than
amorphous gels. This dispersion can be recirculated through the flow reactor for the time
required to complete the reaction; (ii) Unlike the beads, a polyHIPE remains as a stationary
photosensitizer within a glass column where the reagents flow through the porous structure
whilst being irradiated by an external light source. This results in the continuous production of
the target product as long as the polyHIPE remains photoactive.
Heterogeneous photosensitization at 420 nm with Bead-BTZ and pHIPE-BTZ in a
commercial flow reactor. The freely dispersible beads were investigated both in batch as well
as under flow conditions. A batch experiment was set up identical to that of Gel (3%) to
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compare the production of O2. H NMR spectroscopy revealed similar conversion rates of αꢀ
terpinene to ascaridole compared to that of Gel (3%) (>95%) (Figure S25). This confirmed the
photosensitizing nature of Bead-BTZ and showed no appreciable negative effects due to the
added DVBꢀ55.
A continuous flow experiment was then carried out with Bead-BTZ with the experimental set
up depicted in Figure 5a. The dispersion was recirculated through the photochemical reactor for
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60 minutes where full conversion to ascaridole was observed via H NMR spectroscopy (Figure
S26). The production rate for the conversion of αꢀterpinene to ascaridole was found to be 136
mg.hꢀ1. In order to relate this to the amount of photoactive component in the beads, the
production rate of ascaridole per mg of St-BTZ in Bead-BTZ needs to be evaluated. This
corresponds to 680 mg.hꢀ1.
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pHIPE-BTZ was also employed as a photosensitizer under continuous flow conditions.
However, the photochemical reactor was replaced with the column containing the trapped
photoactive polyHIPE, as described above (Figure 2b). Due to the reduced contact time with
pHIPE-BTZ, some experimental conditions had to be changed: (i) The concentration of the
solution was lowered to 0.4 mmol αꢀterpinene in 10 mL of chloroform; (ii) air was replaced with
pure oxygen; (iii) flow rates of both the reaction mixture and oxygen were reduced to 0.5
mL.minꢀ1 (Figure 5b). Similarly to the gels, pHIPE-BTZ did swell in chloroform. Therefore, the
polyHIPE samples were allowed to swell for 16 h in chloroform prior to the introduction of a
reaction mixture. These changes were made to allow for a greater opportunity to yield full
conversion to ascaridole after a single pass through the irradiated pHIPE-BTZ column.
Figure 5. Schematic representation of the experimental setꢀup using the easyꢀPhotochem flow
reactor from Vapourtec Ltd. for a) Bead-BTZ and b) pHIPE-BTZ.
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Under the stated conditions, we observed full conversion of αꢀterpinene to ascaridole after a
single pass of the 10 mL solution of αꢀterpinene (0.4 mmol in chloroform) (Figure S27). To
investigate the reusability of pHIPE-BTZ, a continuous stream of the reaction mixture was
allowed to flow through the column, taking measurements in 10 mL increments. After 30
measurements under continuous flow and irradiation, no decrease in the conversion rate was
observed (Figure S28). This suggests that the pHIPE-BTZ photosensitizer is stable to both light
and 1O2 over extended usage periods. Moreover, we note that the same column was employed in
the photosensitization of O2˙ꢀ for the aerobic hydroxylation of aryl boronic acids (as described
below), demonstrating the superior stability of pHIPE-BTZ and its potential as a reusable
photoactive material.
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The production rate for the conversion of αꢀterpinene to ascaridole under pHIPE-BTZ
conditions was found to be 192 mg.hꢀ1. Thus, both of the continuous flow methods provide
specific benefits. The use of Bead-BTZ in flow results in good production rates where larger
quantities of material are required. However, the use of pHIPE-BTZ comprises a method in
which no intervention is required to obtain the desired product, such as recovery of the polymer
from the reaction mixture. These results demonstrate the potential of these stable and reusable
materials in heterogeneous photocatalytic applications.
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Production of O2 through photosensitization reactions under sunlight irradiation. To
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demonstrate further the potential and robustness of these new polymers, a O2 production
experiment was carried out in batch with Gel (3%) and Bead-BTZ under direct sunlight
irradiation as an alternative to the 420 nm LED modules. All other parameters were kept the
same as described above for the batch reactions. The experimental setꢀup (Figure 6)
demonstrates the ease with which the experiment was carried out. Full conversion was observed
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after 90 minutes with Gel (3%) and after 60 minutes with Bead-BTZ (Figure S29 and S30).
Under natural sunlight irradiation and aerobic conditions, along with comparable conversion
rates, we were able to validate the robustness of the synthesized photosensitizers. The ability to
use sunlight as a free source of energy and air as the source of oxygen demonstrates how two of
the main components for this photosensitization reaction greatly increase the potential for low
environmental impact applications.
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Figure 6. Experimental setꢀup of the conversion of αꢀterpinene to ascaridole using 1O2 produced
from direct sunlight irradiation.
Aerobic oxidative hydroxylation of aryl boronic acids. Due to the ability of the described
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polymers to reach a stable excited triplet state for the generation of O2, we successfully
demonstrated how these materials compare to our previous work.27ꢀ29 This prompted
investigations into the potential of these polymers to act as triplet photosensitizers in other
chemical transformations. Materials which can occupy an excited triplet state and subsequently
be employed as photocatalysts have been studied in depth and are well known.8 Within the
applications for which triplet photosensitizers can be employed, we envisaged the use of our
photoactive polymers for photoredox reactions.
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One such reaction that has received much attention recently is the aerobic hydroxylation of
aryl boronic acids to phenols. Xiao et al. first reported the use of a visibleꢀlight photocatalytic
strategy to convert aryl boronic acids to phenols using Ruꢀbased complexes and organic dyes.42
The large scale synthesis of phenols is of great importance as they have wide applications in the
chemical and pharmaceutical industries. However, the photosensitizers employed for this process
are largely transition metalꢀbased materials, which can be problematic when trace amounts of
these metals remain in the reaction products, as noted previously. More recently, Zhang et al.
reported the use of porous carbazole networks as effective visibleꢀlight heterogeneous
photocatalysts for organic synthesis, including the transformation of aryl boronic acids to
phenols.43 This demonstrates the growing interest for metalꢀfree, heterogeneous photocatalytic
materials. In this context, we implemented the synthesized polymers as heterogeneous
photosensitizers for the aerobic oxidative hydroxylation of aryl boronic acids.
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A standard model reaction was first performed for the oxidation of phenylboronic acid,
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demonstrating full conversion via H NMR spectroscopic analysis (see ESI) after 24 h. A series
of control experiments were then performed, eliminating either light, air, base or the
photocatalyst (Table S6). Each of these controls showed that no reaction occurred, indicating the
essential requirement of each component for this photocatalytic reaction. St-BTZ was employed
as a monomeric comparison to Gel (5%). While photoactive, the monomer was unable to fully
convert phenylboronic acid to phenol within 24 h and was therefore less efficient. The focus of
this model reaction then turned to optimization of the reaction condition. Upon utilization of
different solvents (MeOH, CH3CN) and bases (Et3N, 1,8ꢀdiazabicyclo[5.4.0]undecꢀ7ꢀene), we
discovered that the initial reaction conditions resulted in the swiftest time to full conversion of
the boronic acid.
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A scope of reagents for aerobic oxidative hydroxylation of aryl boronic acids is summarized in
Table 1. Using the initial optimized reaction conditions, a range of substituted aryl boronic acids
were oxidized to the corresponding aryl alcohols until full conversion was demonstrated. A range
of electronꢀneutral, electronꢀdonating and electronꢀwithdrawing substituents were successfully
converted to the alcohols. We discovered that among these substrates, those with electronꢀ
donating properties (Table 1, Entry 2ꢀ3) showed slower conversion rates (40ꢀ72 h) compared to
phenyl boronic acid. However, those with strong electronꢀwithdrawing substituents (Table 1,
Entry 4ꢀ6) showed markedly increased conversion rate (18 h) when compared to the other
groups. One reagent that did not follow this trend was the esterꢀsubstituted phenyl boronic acid
(Table 1, Entry 5), showing full conversion after 48 h. Within this scope, we also extended the
use of these reaction conditions for the conversion of phenyl boronic pinacol ester, a derivative
of phenyl boronic acid, to phenol. To our surprise, full conversion to the phenol product was
seen after 15 h, nearly half the time required for the conversion of phenyl boronic acid (Table 1,
Entry 7).
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Table 1. Light induced aerobic oxidative hydroxylation of aryl boronic acids.a
Conversion (%)b
Entry Photosensitizer Ar
Time (h)
Gel (5%)
Gel (5%)
Gel (5%)
24
>99
>99
>99
1
2
3
72
40
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Gel (5%)
Gel (5%)
18
48
>99
>99
4
5
9
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Gel (5%)
18
15
>99
>99
6
7
Gel (5%)
8c
9
pHIPEꢀBTZ
30
10
5
>99
>99
>99
BeadꢀBTZ
(Batch)
10c
BeadꢀBTZ
(Flow)
aReaction conditions: Aryl boronic acid (0.5 mmol/5 mL DMF), Gel (5%) (5 mg), DIPEA (1.0
b
1
mmol), DMF (5 mL), 420 nm LED irradiation, air, 24 h. Conversion calculated via H NMR
spectroscopy. DIPEA = N,N’ꢀdiisopropylethylamine, DMF = N,N’ꢀdimethylformamide.
cReaction performed under flow conditions.
Investigations into the mechanism of this reaction have been discussed within the literature and
we believe the above reactions proceed in a similar fashion, through the generation of superoxide
radicals via an electron transfer mechanism between the triplet photosensitizer and an amine
base.42, 43 By removing the base and subsequent source of superoxide radicals, we have shown
that 1O2, which we know is generated, has no effect on the aryl boronic acids. However, we have
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not found any experimental studies within the literature that investigate the generation of O2
along with O2˙ꢀ in the presence of a base. To test this, αꢀterpinene, which is well known to react
only with 1O2,36 was added to the model reaction. The resultant H NMR spectrum (Figure S45)
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showed the presence of both the phenol and ascaridole products, confirming the concomitant
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production O2 and O2˙ꢀ in the same reaction. While this may not have any particular benefit
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regarding synthetic procedures, it is important to recognize as a potential issue when reactants or
products are particularly susceptible to an unwanted attack by 1O2.
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As a further showcase of the synthesized polymers as versatile heterogeneous triplet
photosensitizers, we implemented the optimized model reaction under flow conditions,
employing both pHIPE-BTZ and Bead-BTZ. The use of the immobilized pHIPE-BTZ resulted
in a time of 30 h to reach full conversion (Table 1, Entry 8). While this is a significantly longer
reaction time when compared to the model reaction in batch, we note that the total usage of the
photoactive polyHIPE at this point was around 80 h. During this time, the polyHIPE was
exposed to direct light irradiation, a variety of solvent and chemical systems and harsh oxidative
conditions. The fact that pHIPE-BTZ continued to display good photocatalytic activity after
such an extended and extreme use illustrates the robust and nearly indestructible nature of the
material. Implementation of Bead-BTZ under both batch and flow conditions was also
performed, both resulting in unsurprisingly accelerated conversion rates. Under batch conditions
the optimized model reaction required 10 h to show full conversion to the phenol product, more
than halving the time required when employing Gel (5%) as the photosensitizer. Under flow
conditions, the same reaction showed yet another dramatic decrease in reaction time with full
1
conversion occurring after only 5 h. When comparing these results to those of O2 generation, a
large disparity can be seen with regards to the photoactive capabilities of the polymers under
different reaction conditions. While the gels and beads both showed comparable reaction rates
1
for O2 generation, we now see a vast difference in effectiveness for the photoredox reaction.
This is most likely due to dissimilar physical characteristics between the two materials. While
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the gels are able to swell greatly, they do not disperse as well as the beads in the same solvent
systems. The shape and size of Bead-BTZ aids in this as they form well distributed suspensions,
which can create a larger surface area by which triplet state interactions can occur. This idea can
also be applied to the decreased reaction time required for continuous flow over batch
conditions. A suspension can cause light penetration difficulties, resulting in areas of the reaction
mixture receiving lower flux and therefore a decrease in triplet state interactions. By applying the
same reaction in flow, we negated this issue as demonstrated by the change in the rate of
reaction.
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Lastly, due to the enhanced capabilities of Bead-BTZ, we aimed to test both the reusability
and productivity of the beads under flow conditions using the same model reaction. We found
that beads showed good reusability, with full conversion occurring within the same time as the
first run (5 h) after six subsequent uses. Furthermore, and as explained previously, the pHIPE-
BTZ monolith was used over 30 times for the production of 1O2 and then employed in the light
induced aerobic oxidative hydroxylation of aryl boronic acids, showing great photostability,
stability towards reactive oxygen species, and that it can be reused easily for both of the
reactions. The productivity of the material was investigated by determining the maximum
concentration that can be implemented where full conversion was still achieved after 5 h. From a
starting concentration of 0.1 mmol.mLꢀ1, we successfully increased this to 0.4 mmol.mLꢀ1 before
longer reaction times were required. The dramatic reduction in reaction time, along with
reusability and productivity studies, further validates the use of continuous flow technology as a
tool for enhancing and optimizing reaction protocols even in heterogeneous photocatalysis.
CONCLUSION
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