Preparation of oligonucleotides containing 5-bromouracil and 5-methylcytidine

Article abstract of DOI:10.1080/07328319608002137

A previously described side reaction on 5-bromouracil during standard oligonucleotide deprotection conditions has been studied in detail. The side product, 5-amino-2′-deoxyuridine, is isolated and characterized. The use of several 5-methylcytidine protected derivatives for the preparation of oligonucleotides containing 5-bromouracil and 5-methylcytidine free of 5-amino-2′-deoxyuridine is discussed.

Full text of DOI:10.1080/07328319608002137

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Preparation of Oligonucleotides
Containing 5-Bromouracil and 5-
Methylcytidine.
E. Ferrer a b , C. Fàbrega a b , R. G. Garcia b , F. Azorín a & R.
Eritja a b
a Centro de Investigación y Desarrollo , CSIC. Jordi Girona 18,
Barcelona, 08034, Spain
b European Molecular Biology Laboratory (EMBL). ,
Meyerhofstrasse 1, D-69117, Heidelberg, Germany
Published online: 20 Aug 2006.
To cite this article: E. Ferrer , C. Fàbrega , R. G. Garcia , F. Azorín & R. Eritja (1996) Preparation
of Oligonucleotides Containing 5-Bromouracil and 5-Methylcytidine., Nucleosides and Nucleotides,
15:4, 907-921, DOI: 10.1080/07328319608002137
To link to this article: http://dx.doi.org/10.1080/07328319608002137
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