TERPENOID INDOLE BIOTRANSFORMATION CAPACITY OF SUSPENSION CULTURES OF TABERNAEMONTANA DIVARICATA

Article abstract of DOI:10.1016/S0031-9422(00)90584-3

Two cell lines of Tabernaemontana divaricata derived from the same suspension culture were compared with respect to their biotransformation capacity.One is high indole alkaloid-producing culture wich accumulated mainly O-acetylvallesamine.The other cell line biosynthesizes terpenoid indole alkaloids in much lower amounts.Both cell lines were cultured in medium containing either conopharyngine, coronaridine, vobasine or tabersonine.Chemical breakdown was followed in fresh and the used culture medium in the absence of cell culture.All the alkaloids investigated underwent chemical transformation.Most of the biotransformation products accumulated by the cultures have been reported to occur in intact plants.Since all the alkaloids added were transformed in the same way by both cultures, the two cell lines seem to have the same biotrasformation potential.