Encapsulated energy-transfer cassettes with extremely well resolved fluorescent outputs

Article abstract of DOI:10.1021/ja107193j

This paper concerns the development of water-compatible fluorescent imaging probes with tunable photonic properties that can be excited at a single wavelength. Bichromophoric cassettes 1a-1c consisting of a BODIPY donor and a cyanine acceptor were prepare

Full text of DOI:10.1021/ja107193j

Published on Web 11/24/2010
Encapsulated Energy-Transfer Cassettes with Extremely Well
Resolved Fluorescent Outputs
Yuichiro Ueno,† Jiney Jose,† Aurore Loudet,† Ce´sar Pe´rez-Bol´ıvar,‡
Pavel Anzenbacher, Jr.,‡ and Kevin Burgess*,†
Departments of Chemistry, Texas A & M UniVersity, Box 30012, College Station, Texas 77841,
United States, and Bowling Green State UniVersity, Bowling Green, Ohio 43403, United States
Received August 10, 2010; E-mail: burgess@tamu.edu
Abstract: This paper concerns the development of water-compatible fluorescent imaging probes with tunable
photonic properties that can be excited at a single wavelength. Bichromophoric cassettes 1a-1c consisting
of a BODIPY donor and a cyanine acceptor were prepared using a simple synthetic route, and their
photophysical properties were investigated. Upon excitation of the BODIPY moiety at 488 nm the excitation
energy is transferred through an acetylene bridge to the cyanine dye acceptor, which emits light at
approximately 600, 700, and 800 nm, i.e., with remarkable dispersions. This effect is facilitated by efficient
energy transfer that gives a “quasi-Stokes” shift between 86 and 290 nm, opening a huge spectral window
for imaging. The emissive properties of the cassettes depend on the energy-transfer (ET) mechanism: the
faster the transfer, the more efficient it is. Measurements of rates of ET indicate that a through-bond ET
takes place in the cassettes 1a and 1b that is 2 orders of magnitude faster than the classical through-
space, Fo¨rster ET. In the case of cassette 1c, however, both mechanisms are possible, and the rate
measurements do not allow us to discern between them. Thus, the cassettes 1a-1c are well suited for
multiplexing experiments in biotechnological methods that involve a single laser excitation source. However,
for widespread application of these probes, their solubility in aqueous media must be improved.
Consequently, the probes were encapsulated in calcium phosphate/silicate nanoparticles (diameter ca. 22
nm) that are freely dispersible in water. This encapsulation process resulted in only minor changes in the
photophysical properties of the cassettes. The system based on cassette 1a was chosen to probe how
effectively these nanoparticles could be used to deliver the dyes into cells. Encapsulated cassette 1a
permeated Clone 9 rat liver cells, where it localized in the mitochondria and fluoresced through the acceptor
part, i.e., red. Overall, this paper reports readily accessible, cyanine-based through-bond ET cassettes
that are lypophilic but can be encapsulated to form nanoparticles that disperse freely in water. These particles
can be used to enter cells and to label organelles.
from ground to excited states;5,6 for most planar, conjugated
Introduction
organic labels, which have relatively small Stokes’ shifts (10-20
nm),7 this parameter is not easily manipulated.8,9 Thus, multi-
plexed fluorescent labels for excitation at one wavelength must
involve compromise between two opposing physical param-
eters.3 If the excitation source is set at the absorption maxima
of the dyes used, then all the dyes must have nearly the same
absorption maxima, and the limit of the resolution is defined
by the Stokes’ shifts of the dyes. Conversely, if the dyes are
chosen for their diverse fluorescence emission maxima, then
the UV absorption maxima of some of the dyes will not
correspond to the excitation wavelength, these dyes will absorb
less light, and they will fluoresce less brightly.
Fluorescent labels that display high photostability and chemi-
cal stability, bright fluorescence, and emission wavelength
tunability are important tools in cellular biology.1,2 Such labels
generally allow for high-quality images at lower photon flux,
without sacrificing accuracy (low background). If the labels
allow channel multiplexing, then that is also an advantage, as
it facilitates tracking of several components in a single
experiment.3,4 However, large Stokes’ shifts are required to
achieve resolutions necessary for multiplexing experiments.
These shifts correspond to energies required for reorganization
(5) Delmotte, C.; Delmas, A. Bioorg. Med. Chem. Lett. 1999, 9, 2989–
2994.
† Texas A & M University.
‡ Bowling Green State University.
(6) Taylor, D. L.; Haskins, J. R.; Giuliano, K. A. High Content Screening:
A Powerful Approach to Systems Cell Biology and Drug DiscoVery;
Humana Press: Totowa, NJ, 2007; Vol. 356.
(1) Sauer, M.; Hofkens, J.; Enderlein, J. Handbook of Fluorescence
Spectroscopy and Imaging: From Ensemble to Single Molecules;
Wiley-VCH: Weinheim, 2010.
(2) Sabnis, R. W. Handbook of Biological Dyes and Stains. Synthesis and
Industrial Applications; Wiley & Sons: Hoboken, NJ, 2010.
(3) Levenson, R. M.; Mansfield, J. R. Cytometry 2006, 69, 748–758.
(4) Haney, S. A. High Content Screening: Science, Techniques and
Applications; Wiley-Interscience: Hoboken, NJ, 2008.
(7) Montalti, M.; Credi, A.; Prodi, L.; Gandolfi, M. T. Handbook of
Photochemistry, 3rd ed.; CRC Press-Taylor & Francis: Boca Raton,
FL, 2006.
(8) Wang, L.; Tan, W. Nano Lett. 2006, 6, 84–88.
(9) Lakowicz, J. R. Principles of Fluorescence Spectroscopy, 3rd ed.;
Springer: New York, 2006.
9
10.1021/ja107193j  2011 American Chemical Society
J. AM. CHEM. SOC. 2011, 133, 51–55 51

A R T I C L E S
Ueno et al.
Scheme 1. Syntheses of the Through-Bond Energy-Transfer
Cassettes 1a-1c
Many applications in bioimaging require probes that are
compatible with aqueous media. Incorporation of water-solu-
bilizing groups in labels is a challenge10 and presents problems
during purification,5 especially since even small amounts of
fluorescent impurities may skew the data obtained in imaging
experiments. Furthermore, water-solubilizing groups attached
to fluors often result in decreased fluorescence intensities due
to nonradiative decay processes facilitated by solvation sphere
rearrangement in the excited state.11,12 Finally, if these obstacles
are overcome, the resulting labels may be highly polar; this tends
to impart an increased affinity for cytoplasm, making the probes
less useful for imaging of other cellular compartments.
Here we present a rational design of fluorescent labels that
allows tuning of fluorescent outputs through a wide emission
window via cassettes composed of donor and acceptor
chromophores.8,11,13,14 Specifically, this paper describes cassettes
that work via excitation of a BODIPY-based15-18 donor with
blue light (e.g., 488 nm) followed by fast energy transfer (ET)
to variable cyanine-based19,20 acceptors, which then emit red
light. Three cassettes were prepared, 1a-1c (Scheme 1). The
wavelength of the emitted light depends on the structure of the
acceptor and varies from 590 to 794 nm. While the Stokes’
shift of the donor is short (∼20 nm), the red shift in the cassettes
is between 86 and 290 nm; this dispersion is greater than any
other achieved in cassettes generated in these laboratories.21-29
These probes are relatively easy to make, partly because they
are lipophilic, but they have poor water solubilities. To obviate
this issue, the cassettes were encapsulated in calcium phosphate/
silicate nanoparticles that are freely dispersed in water. Experi-
ments are described to elucidate how these particles can be used
as delivery agents wherein the dye-containing particles become
localized within the cells.
Results and Discussion
(10) Romieu, A.; Brossard, D.; Hamon, M.; Outaabout, H.; Portal, C.;
Renard, P.-Y. Bioconjugate Chem. 2008, 19, 279–289.
(11) Fo¨rster, T. Naturwissenschaften 1946, 6, 166–175.
(12) Laia, C. A. T.; Costa, S. M. B. Chem. Phys. Lett. 1998, 285, 385–
390.
Syntheses and Spectroscopic Properties of the Cassettes.
Cassettes 1a-1c were prepared by cross-coupling the readily
available donor fragment D with the iodine-functionalized
cyanine dyes 2a-2c (Scheme 1 and Supporting Information).
These materials were easily purified via flash chromatography
because they are lipophilic and colored.
Spectroscopic data for the cassettes 1 are presented in Figure
1 and Table 1. Figure 1a shows the absorbance spectra; these
resemble the summation of components from the donor and
acceptor fragments. When the cassettes are excited at 504 nm
(the donor), 1a and 1b emit predominantly from their acceptor
fragments; only about 10% of the fluorescence “leaks” from
the donor part. Leakage from the donor is prevalent in the
fluorescent spectra of 1c under the same conditions. Quantita-
tively, this parameter is reflected by the energy-transfer ef-
ficiencies (ETEs; {Φd/Φa} × 100%)29 of these cassettes (1a
and 1b, >88%; 1c, 43%).
We speculate that the difference in quantum yields for the
three cassettes after encapsulation is due to the orientation of
the cassettes within the particles. In the case of cassette 1a (Cy3
cassette), the fluor is well encapsulated and devoid of any
π-stacking; hence, its quantum yield is high. However, 1b and
1c may be oriented in ways that favor more π-stacking, resulting
in lower quantum yields.
Ultrafast Spectroscopy Measurements. Ethynylene linkers
were incorporated in cassettes 1a-1c to facilitate donor-to-
acceptor ET through bonds,30 though through-space ET is also
plausible. Femtosecond transient spectroscopy was performed
to compare actual rates of ET in these systems to ones calculated
(13) Zhu, L.; Soper, S. A. ReV. Fluoresc. 2006, 3, 525–574.
(14) Lakowicz, J. R. Principles of Fluorescence Spectroscopy, 2nd ed.;
Kluwer Academic/Plenum Publishers: New York, 1999.
(15) Loudet, A.; Burgess, K. In Handbook of Porphyrin Science: With
Applications to Chemistry, Physics, Materials Science, Engineering,
Biology and Medicine; Kadish, K., Smith, K., Guilard, R., Eds.; World
Scientific: Singapore, 2010; p 203.
(16) Ulrich, G.; Ziessel, R.; Harriman, A. Angew. Chem., Int. Ed. 2008,
47, 1184–1201.
(17) Ziessel, R.; Ulrich, G.; Harriman, A. New J. Chem. 2007, 31, 496–
501.
(18) Loudet, A.; Burgess, K. Chem. ReV. 2007, 107, 4891–4832.
(19) Mishra, A.; Behera, R. K.; Behera, P. K.; Mishra, B. K.; Behera, G. B.
Chem. ReV. 2000, 100, 1973–2011.
(20) Ozhalici-Unal, H.; Pow, C. L.; Marks, S. A.; Jesper, L. D.; Silva,
G. L.; Shank, N. I.; Jones, E. W.; Burnette, J. M., III; Berget, P. B.;
Armitage, B. A. J. Am. Chem. Soc. 2008, 130, 12620–12621.
(21) Burgess, K.; Burghart, A.; Chen, J.; Wan, C.-W. Proc. SPIE-Int. Soc.
Opt. Eng. 2000, 3926, 95–105.
(22) Burghart, A.; Thoresen, L. H.; Chen, J.; Burgess, K.; Bergstrom, F.;
Johansson, L. B.-A. Chem. Commun. 2000, 2203–2204.
(23) Jiao, G.-S.; Thoresen Lars, H.; Burgess, K. J. Am. Chem. Soc. 2003,
125, 14668–14669.
(24) Wan, C.-W.; Burghart, A.; Chen, J.; Bergstroem, F.; Johansson,
L. B. A.; Wolford, M. F.; Kim, T. G.; Topp, M. R.; Hochstrasser,
R. M.; Burgess, K. Chem.-Eur. J. 2003, 9, 4430–4441.
(25) Burgess, K. (The Texas A&M University System). U.S. Patent
0032120 A1, 2005.
(26) Bandichhor, R.; Petrescu, A. D.; Vespa, A.; Kier, A. B.; Schroeder,
F.; Burgess, K. J. Am. Chem. Soc. 2006, 128, 10688–10689.
(27) Kim, T. G.; Castro, J. C.; Loudet, A.; Jiao, J. G. S.; Hochstrasser,
R. M.; Burgess, K.; Topp, M. R. J. Phys. Chem. A 2006, 110, 20–27.
(28) Jose, J.; Ueno, Y.; Wu, L.; Loudet, A.; Chen, H.-Y.; Son, D. H.;
Burgess, K. Chem. Commun. 2009, submitted.
(29) Wu, L.; Loudet, A.; Barhoumi, R.; Burghardt, R. C.; Burgess, K. J. Am.
Chem. Soc. 2009, 131, 9156–9157.
(30) Polyansky, D. E.; Danilov, E. O.; Voskresensky, S. V.; Rodgers,
M. A. J.; Neckers, D. C. J. Am. Chem. Soc. 2005, 127, 13452–13453.
9
52 J. AM. CHEM. SOC. VOL. 133, NO. 1, 2011

Cyanine-Based Through-Bond Energy-Transfer Cassettes
A R T I C L E S
Figure 2. Spectral overlap of BODIPY donor emission (black line) and
cassette absorption (1a, red line; 1b, green line; 1c, blue line) ensures
resonance energy transfer.
rates calculated for through-space ET using the Fo¨rster model
(Table 2). From the perspective of the resonance energy transfer
(RET) theory, the overlap integral J for the emission of the donor
(common for the three cassettes) and the absorption of the
indocyanine acceptor (Figure 2) show a stepwise decrease 1a
> 1b > 1c, while the Fo¨rster radius (R0) increases. Following
the Fo¨rster model, the calculated theoretical value for the RET
decreases from 8.59 × 109 for 1a to 1.88 × 109 for 1b and
1.39 × 109 for 1c (Table 2). Consequently, the Fo¨rster ET rate
calculated for cassette 1a, for instance, was approximately 4
times faster than the observed rate for the intermolecular transfer
in the control described above.
Figure 1. Normalized (a) absorbance and (b) fluorescence spectra of the
cassettes in EtOH (at 10-6 and 10-7 M for absorbance and fluorescence
measurements, respectively).
Table 1. Photophysical Properties of Cassettes
λabs (nm)
λem (nm)
Φda
Φab
ETE (%)
In EtOH
1a
1b
1c
504, 569
504, 662
504, 763
519, 590
519, 687
519, 794
0.20(0.01
0.35(0.02
0.11
0.22(0.02
0.40(0.03
90
87
c
-
43d
Observed ET rates for cassettes 1a and 1b were 2 orders of
magnitude higher than the rates predicted for the Fo¨rster model.
Specifically, the rates for 1a and 1b are 56 and 67 times faster
than the ones calculated for through-space ET (Table 2). These
data suggest a dramatic effect of the donor-acceptor com-
munication through the phenylethyne bridge.
Cassette 1c shows an ET rate equal (within 10% error) to
the through-space ET rate following the Fo¨rster model. This
suggests that in 1c the RET proceeds through-space or in a
mixed through-space and through-bond mechanism. Overall, the
measured rates suggest the efficiency decreases from 90 to 87
and 43% for 1a, 1b, and 1c, respectively.
On a picosecond time scale, ET was observed by following
the concerted kinetics of the donor-stimulated emission decay
after the excitation and the population of the acceptor excited
state following the bleaching of the donor. After the energy is
transferred, the acceptor decays at its usual time scale, i.e., as
if excited directly. For example, excitation of cassette 1a at the
donor (420 nm) by a femtosecond laser pulse is followed by
the decay of a singlet state, which is complete within 2.1 ps
and is accompanied by the population of an acceptor excited
Nanoparticles in pH 7.4 Phosphate Buffer
1a
1b
1c
504, 568
504, 659
504, 764
519, 592
519, 687
519, 793
0.26(0.01
0.063
0.053
0.29(0.02
88
0.071
89
c
-
41d
a Quantum yield of acceptor when excited at donor relative to
rhodamine 6G (Φ ) 0.92 in EtOH). b Quantum yield of acceptor when
excited at acceptor relative to rhodamine 101 for 1a (Φ ) 1.0 in EtOH)
and Nile Blue for 1b (Φ ) 0.27 in EtOH). c No appropriate standard
due to range of wavelengths. d See Supporting Information for
calculation. Quantum yields were measured three times and averaged,
and they were corroborated using the absolute fluorescence quantum
yield measurements. ETE, energy transfer efficiency, calculated as
(Φd/Φa) × 100%.
for transfer through space (fluorescence resonance energy
transfer, FRET). As a control, intermolecular ET between the
donor D in ethanol and an equimolar (3.1 × 10-6 M)
concentration of the non-iodinated analogues of the acceptors
(2a, Cy3) was also studied. Energy transfer did occur under
these conditions, at a rate of 1.85 × 109 s-1
(at the concentration of 3.1 × 10-6 M) and compared with the
.
Energy transfer rates were measured for the cassettes 1a-1c
Table 2. Energy-Transfer Data Calculated and Recorded Using Time-Resolved Fluorescence Spectroscopy and Femtosecond Transient
Spectroscopy in Ethanol at 22 °C
transfer rate
measured (s-1
Fo¨rster radius
calcd R0 (Å)
overlap integral J
(M-1 cm3 nm4)
transfer rate
a
measured ET
efficiency (%)
cassette
)
calcd (s-1
)
donor decay (ps)
acceptor raise (ps)
acceptor decay (ps)
1a
1b
1c
4.85 × 1011
1.26 × 1011
1.26 × 109
129
100
94
3.47 × 1017
7.61 × 1016
5.14 × 1016
8.59 × 109
1.88 × 109
1.39 × 109
90
87
43
2.06
7.96
794
2.18
9.55
NDb
352
∼1000
719
a Transfer rate for the Fo¨rster through-space ET was calculated using r (donor-acceptor distance), determined as an average center-to-center distance
using kT(r) ) R0 /τD, ꢀ2 ) 2/3, r6 formula, and r ) 66.41 Å. b Relatively fast population of the acceptor singlet excited state occurred due to the direct
6
pumping of the acceptor absorption by the laser (1c, ε420 ) 9800 M-1 cm-1).
9
J. AM. CHEM. SOC. VOL. 133, NO. 1, 2011 53

A R T I C L E S
Ueno et al.
Figure 3. (Left) Transient absorption spectra of the cassette 1a showing a concerted depletion of the stimulated emission donor maximum at 505 nm (blue
arrow) and raise of the acceptor maxima at 580 nm (red arrow), followed by acceptor relaxation within 350 ps. (Right) Kinetic profiles of the donor decay
(2.06 ps) and acceptor raise (2.18 ps) reflect the concerted process.
Figure 4. Atomic force microscopy images of cassettes encapsulated in calcium phosphate nanoparticles. Average particle size, 22 nm.
state with 2.2 ps raise time. Thereafter, the acceptor decays with
a fluorescence lifetime of 352 ps by emitting with a λmax of
600 nm (Figure 3). Similarly, exciting cassette 1b at 420 nm
results in singlet-state population of the BODIPY donor, decay
within 8.0 ps, and then complete population of the acceptor
excited state after 9.6 ps; the acceptor singlet excited state decays
with a fluorescence lifetime of ∼1 ns (λmax ) 680 nm). In
contrast to the other two cassettes, the donor of 1c decays slowly
(within 800 ps), which suggests that the excited state of the
donor is not being depopulated by a strongly coupled acceptor
as it is in the cassettes 1a and 1b, and the ET is less efficient.
Through-space RET is still operational in the cassette 1c since
the donor alone decays with a lifetime of 4 ns, which is
comparable to the 800 ps rate in the cassette.
with citrate-derived surface carboxylate groups and fluorescent
dye cargoes.38 They have investigated these particles for imaging
in cells and in ViVo.39-43
In our work, calcium phosphate/silicate nanoparticles encap-
sulating cassettes 1a-1c were prepared via Adair’s procedure38
except that a longer reaction time was used (24 h, not 5 min),
the particles were purified via medium-pressure rather than high-
pressure liquid chromatography, and finally a dialysis step was
performed to transfer the particles from an ethanolic to an
aqueous medium. Figure 4 shows atomic force microscopy
(31) Davis, W. B.; Svec, W. A.; Ratner, M. A.; Wasielewski, M. R. Nature
1998, 396, 60–63.
The following conclusions can be drawn from the measure-
ment of ET rates. First, a significantly more efficient pathway
for donor-acceptor communication exists for cassettes 1a and
1b compared to that in 1c. Aryl-ethynyl-aryl moieties display
a strong electronic coupling,30 and this suggests that the ET
takes place through the bridge. The efficiency and rate of the
ET processes in 1a and 1b are a strong indication of an exciton
hopping mechanism.31-34 Cassette 1c transfers energy at a rate
that can be rationalized using the Fo¨rster through-space model.
Encapsulation of the Dyes in Calcium Phosphate/Silicate.
Cassettes 1a-1c are not water-soluble, but we show here that
they can be encapsulated in calcium phosphate to form
nanoparticles. With respect to biotechnological applications,
calcium phosphate is an excellent matrix for nanoparticle
encapsulation because (i) moderate concentrations of Ca2+ ions
are not toxic to cells; (ii) it is not toxic in ViVo (found in human
bone and teeth); (iii) it is claimed that calcium phosphate
dissolves below pH 5.5 to liberate the cargo but is stable at
7.4;35 (iv) particles of this matrix disperse freely in aqueous
media; and (v) the surface of these particles can be functional-
ized.36,37 Adair and co-workers have shown that spherical, ca.
18 nm diameter calcium phosphate nanoparticles can be formed
(32) Kim, D.; Osuka, A. Acc. Chem. Res. 2004, 37, 735–745.
(33) Montes, V. A.; Perez-Bolivar, C.; Estrada, L. A.; Shinar, J.;
Anzenbacher, P., Jr. J. Am. Chem. Soc. 2007, 129, 12598–12599.
(34) Montes, V. A.; Perez-Bolivar, C.; Agarwal, N.; Shinar, J.;
Anzenbacher, P., Jr. J. Am. Chem. Soc. 2006, 128, 12436–12438.
(35) Bisht, S.; Bhakta, G.; Mitra, S.; Maitra, A. Int. J. Pharm. 2005, 288,
157–168.
(36) Altinoglu, E. I.; Russin, T. J.; Kaiser, J. M.; Barth, B. M.; Eklund,
P. C.; Kester, M.; Adair, J. H. ACS Nano 2008, 2, 2075–2084.
(37) Morgan, T. T.; Muddana, H. S.; Altinoglu, E. I.; Rouse, S. M.;
Tabakovic, A.; Tabouillot, T.; Russin, T. J.; Shanmugavelandy, S. S.;
Butler, P. J.; Eklund, P. C.; Yun, J. K.; Kester, M.; Adair, J. H. Nano
Lett. 2008, 8, 4108–4115.
(38) Altınog˘lu, E. I.; Russin, T. J.; Kaiser, J. M.; Barth, B. M.; Eklund,
P. C.; Kester, M.; Adair, J. H. ACS Nano 2008, 2075–2086.
(39) Morgan, T. T.; Muddana, H. S.; lu, E. I. A.; Rouse, S. M.; Tabakovic,
A.; Tabouillot, T.; Russin, T. J.; Shanmugavelandy, S. S.; Butler, P. J.;
Eklund, P. C.; Yun, J. K.; Kester, M.; Adair, J. H. Nano Lett. 2008,
8, 4108–4115.
(40) Muddana, H. S.; Morgan, T. T.; Adair, J. H.; Butler, P. J. Nano Lett.
2009, 1559–1566.
(41) Kester, M.; Heakal, Y.; Fox, T.; Sharma, A.; Robertson, G. P.; Morgan,
T. T.; Altinoglu, E. I.; Tabakovic, A.; Parette, M. R.; Rouse, S.; Ruiz-
Velasco, V.; Adair, J. H. Nano Lett. 2008, 8, 4116–4121.
(42) Gupta, R.; Mishra, P.; Mittal, A. J. Nanosci. Nanotechnol. 2009, 9,
2607–2615.
(43) Glowka, E.; Lamprecht, A.; Ubrich, N.; Maincent, P.; Lulek, J.;
Coulon, J.; Leroy, P. Nanotechnology 2006, 17, 2546–2552.
9
54 J. AM. CHEM. SOC. VOL. 133, NO. 1, 2011

Cyanine-Based Through-Bond Energy-Transfer Cassettes
A R T I C L E S
that facilitate energy transfer through bonds. The dispersion of
fluorescence emissions from these cassettes is greater than any
others prepared by us or, to the best of our knowledge, by others.
The observed ET rates for cassettes 1a and 1b are significantly
faster than for dipole-dipole coupling in a through-space
manner, indicating transfer through bonds is indeed occurring.
Overall, they are excellent tools for fluorescence multiplexing
in biotechnology.
Syntheses of such cassettes are straightforward because they
are lipophilic. The idea of using water-dispersible nanoparticles
to bring lipophilic cassettes into aqueous media, thereby
circumventing syntheses of water-soluble materials, is novel and
has the potential to be applied to other donor-acceptor systems
where water-soluble modifications would be hard to make. The
absorbance and emission maxima of the cassettes and the extent
of donor-acceptor energy transfer (ETE) remained significantly
unchanged after encapsulation into nanoparticles. Conversely,
and surprisingly, the fluorescence quantum yields of the three
systems studied in this paper were significantly affected. Thus,
the fluorescence quantum yield for 1a-CaNPs increased by
about 30% relative to the free dye in EtOH, while for 1b-CaNPs
and 1c-CaNPs it decreased by 5- and 2-fold, respectively.
Nanoparticles encapsulating these cassettes may have applica-
tions for intracellular imaging and for observation of parallel
macroscopic events in ViVo, e.g., as labels for targeting different
cancer forms simultaneously, and allow specific targeting of
organelles. Other applications beyond organelle targeting in cells
can now be envisaged.
Figure 5. Fluorescence images of cassette 1a (left) and 1a-CaNP (right)
in normal rat liver cells (Clone 9). Scale bar is 10 µm.
images of the particles formed from the cassettes 1; these are
quite uniform, with diameters around 22 nm.
Spectroscopically, encapsulated cassettes 1a-1c had absorp-
tion and fluorescent spectra that are almost identical to those
of the free cassettes in EtOH (see Table 1). The quantum yield
of encapsulated 1a -CaNP increased about 30% relative to the
free dye in EtOH, but for 1b-CaNP and 1c-CaNP it decreased
by 5- and 2-fold respectively, which is satisfactory for some
applications. Throughout, the ETEs were essentially unchanged.
Permeation of the Particles into Clone 9 Rat Liver Cells.
Cassette 1a and 1a-CaNPs were imported in Clone 9 cells at
37 °C to study their subcellular localization. Cassette 1a was
observed in different organelles: the mitochondria, the lysos-
omes, the endoplasmic reticulum (ER), and the cytoplasm.
Interestingly, the emission output observed was dependent on
the subcellular compartment. Thus, in the mitochondria, perfect
ET was observed; i.e., only emission from the cyanine was seen,
hence the probes fluoresced red. Diffuse green fluorescence was
observed in the cytoplasm and ER and in some bright punctates
(lysosomes); the green fluorescence is indicative of emission
from the donor part. Conversely, 1a-CaNPs localized only in
the mitochondria, and sole emission from the cyanine part was
observed; i.e., the dyes fluoresced red (Figure 5). When the cells
were treated with 1a-CaNPs at 4 °C, no fluorescent signal could
be observed after 2 h incubation, indicating that the nanoparticles
may enter via endocytosis.
Acknowledgment. The TAMU/LBMS-Applications Laboratory
directed by Dr. Shane Tichy assisted with mass spectrometry, and
Prof. A. Tarnovsky and his staff helped with acquiring the transient
spectra. Support for this work was provided by the National
Institutes of Health (GM72041) and by The Robert A. Welch
Foundation (A1121).
Supporting Information Available: Detailed procedure for the
synthesis of the calcium phosphate nanoparticles, their physical
properties, rate of energy transfer calculations, and cell imaging.
This material is available free of charge via the Internet at http://
pubs.acs.org.
Conclusions
The research described here represents the first report of
coupling BODIPY-based donors with cyanine acceptors in ways
JA107193J
9
J. AM. CHEM. SOC. VOL. 133, NO. 1, 2011 55