JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
523
Kinetic study
Funding
Compound 6g was selected for further kinetic study since it had The present investigation was supported by “Science and
the strongest inhibiting activity against AChE among new synthe- Technology Foundation of Huizhou city, Guangdong province”
sized compounds. The graphical analysis of the steady-state inhib- [No. 2014B040008004], “Natural Science Foundation of Hu’nan
ition data of compound 6g is shown in Figure 2 Supplement. province” [No. 2017JJ2050 and No. 2018JJ3572] and the grant of
m
According to the analysis, the increase of K and the decline of Key Laboratory Breeding Base of Hu’nan Oriented Fundamental
V
max with the increasing concentration of compound 6g presents and Applied Research of Innovative Pharmaceutics [No.
the characteristics of mixed-type inhibition. The competitive inhibit 2016TP1029].
constant (K ) of compounds 6g is 7.27 mmol/L, and the non-com-
i
0
petitive constant (K
i
) is 2.14 mmol/L, respectively. This result sug-
gested that compound 6g could simultaneously bind the active
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Log P and pKa assay
Log P was thought as an important physical chemistry parameter
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2
9
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(
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Conclusions
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The unsaturated bond and aromatic ring of cinnamic acid deriva-
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Disclosure statement
1
6. Zhao JY, Song Y, Wang HJ, et al. High performance liquid
The authors have declared no conflict of interest.
chromatographic method for the determination of