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Although we present Mn-PDA-based esterase substrates as a
proof of concept for MRI detection of intracellular analytes,
these contrast agents could also be used for molecular imaging
themselves. In particular, ester derivatives of MnL3 are likely to
be effective reagents for cell labeling and in vivo cell tracking.
Specific labeling of cells expressing PLE or related enzymes
could also provide a pathway to reporter gene mapping by
MRI. Although extracellular esterases might interfere under
some circumstances,28 extracellularly hydrolyzed agents would
not tend to be trapped, and therefore might not contribute
heavily to background signal. Additional targeted or responsive
agents could be constructed by modifying the Mn-PDA
platform. This new family of contrast agents would be most
applicable for molecular imaging applications in animals, but
the stability and relaxivity of Mn-PDA derivatives might
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ASSOCIATED CONTENT
* Supporting Information
The Supporting Information is available free of charge on the
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Detailed methods and further characterization of
synthetic compounds, including Scheme S1, Figures
S1−S9, and Tables S1 and S2 (PDF)
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AUTHOR INFORMATION
Corresponding Author
■
Author Contributions
∥A.B. and B.B.B. contributed equally.
Notes
The authors declare no competing financial interest.
ACKNOWLEDGMENTS
■
The authors thank Atushi Takahashi, Koli Taghizadeh, Emily
Young, and Emily Loucks. Funding came from the MIT Simons
Center and NIH grants R21-MH102470 and U01-NS090451
to A.J., as well as grant P30-ES002109 for the CEHS
Bioanalytical Core.
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