M.W. Sumarah et al. / Phytochemistry 71 (2010) 760–765
765
by monitoring the UV and MS spectral data for each. Compounds 4
Appendix A. Supplementary data
and 5 were isolated by LC–MS–SPE using a triple trap for each. The
SPE cartridges were dried for 30 min with a constant flow of N2.
The compounds were eluted from the SPE cartridges directly into
Supplementary data associated with this article can be found, in
3 mm NMR tubes with 160
lL of CD3CN. The solvent was eventu-
ally changed by evaporation in the NMR tubes using N2 and recon-
stitution with 160 lL of C6D6 for optimized NMR.
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This study was supported by the Industrial Research Assistance
Program (National Research Council of Canada), the Atlantic Inno-
vation Fund and J.D. Irving, Limited. The authors acknowledge the
expertise and contributions of Dr. Kristian Nielsen, Danish Techni-
cal University. Dr. Keith Seifert and Gerry Louis-Seize, AAFC, and
Dr. Christoph Gruenig, Swiss Federal Institute of Technology, for
expertise and interpretation of the DNA sequencing data. Dr. Yves
Aubin for acquisition of the 175 MHz 13C NMR data. John Dedes,
Natural Resources Canada provided helpful advice and assistance
with the C. fumiferana and diet. Hafsa Cherid, Carleton University
and John Nikiforuk, AAFC, provided critical technical support. John
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