3,4-Dihydro-3-methyl-6-nitro-2H-1,3-benzoxazin-2-one, a Reagent for Labeling p-Nitrophenyl Esterases with a Chromophoric Reporter Group - Synthesis and Reaction with Chymotrypsin

Article abstract of DOI:10.1006/bioo.1993.1030

We reported the synthesis of 3,4-dihydro-3-methyl-6-nitro-2H-1,3-benzoxazin-2-one (DMNB), a close structural anlogue of p-nitrophenyl dimethylcarbamate.DMNB is unstable in aqueous solution when exposed to light, but is stable in the dark.The compound reac