1108
X. L. Armesto et al. / Tetrahedron 56 (2000) 1103–1109
Cytobiol. 1993, 31, 71. (k) Cantin, A.; Woods, D. E. J. Clin. Invest.
OD53, 5 mm. The mobile phase was a 72:20:8 mixture of
H2O/0.25 mol dmϪ3 sodium propionate (pHϷ6.5)/
CH3CN at 298.0 K. The fluorescence detection was
carried out with lexcitation360 nm and lemission455 nm:
The minimal fluorescence yield of cysteine/cystine
OPA/MCE derivatives was solved by pre-treatment
with iodoacetic acid.24 The yields of the fluorescent
OPA/MCE amino acid derivatives were quantified by
comparison with the peak produced by homoserine
ꢀtRϷ30:0 min; that was used as internal standard.
Under these conditions, and for the different pH values
used, a mean of 31% yield of Cya ꢀtRϷ5:0 min and 11%
of Cys^Cys ꢀtRϷ12:0 min were obtained after oxidation
of Cys ꢀtRϷ12:0 min with HOCl. However, these yields
must be taken as lower limits, since the stability of the
OPA/MCE derivatives of Cys, Cya and Cys^Cys is not
high, according to the available studies.23b Moreover, it is
worth noting that using this method the peaks corre-
sponding to Cys and Cys^Cys would elute at the same
time, since the latter is reduced to the former.
¨
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The concentration of ClϪ generated after oxidation of Met
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decomposition of the initial oxidation product.
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Acknowledgements
M.I.F. thanks the Xunta de Galicia for a PhD fellowship.
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