ENZYMIC SYNTHESIS OF CHEMICALLY UNSTABLE CARDIAC GLYCOSIDES BY β-GALACTOSIDASE FROM ASPERGILLUS ORYZAE

Article abstract of DOI:10.1016/S0040-4039(01)80221-5

β-Galactosidase obtained from Asp. oryzae was found to have strong transglycosylation activity.The glycosylation of water soluble and insoluble alcohols was successfully performed under such mild conditions at pH 5 at 4-37 deg C by using the enzyme.This enzymic method was applied to the one step synthesis of several cardiac glycosides difficult to obtain by ordinary chemical methods.