Studies on the biosynthesis of corrinoids and porphyrinoids. X. Biosynthetic studies of bacteriochlorophyll-a in Rhodopseudomonas spheroides: Incorporation of 2H-, 13C- and 15N-labeled substrates and origin of the hydrogen atoms

Article abstract of DOI:10.1248/cpb.43.1311

The biosynthetic pathway of bacteriochlorophyll-a in Rhodopseudomonas spheroides was investigated by administration of [2-¹³C]glycine, L-[methyl- ¹³C]methionine, L-[1-¹³C]glutamic acid and ¹³C- or ¹⁵N-labeled δ- aminolevulinic acid (ALA). The origin of the hydrogen atoms was studied by following the incorporation into bacteriochlorophyll of ¹³C-labeled ALA in a medium containing 50% D₂O and that of ²H-, ¹³C-labeled ALA. The labeled bacteriochlorophylls were converted into more stable methyl bacteriopheophorbides, which were subjected to ¹³C-NMR analyses to obtain information about the biosynthetic origin of the hydrogen atoms. The use of ¹³C-enriched bacteriochlorophylls and methyl bacteriopheophorbides allowed us to make ¹³C-NMR signal assignments for all carbons of the porphyrin nucleus.

Full text of DOI:10.1248/cpb.43.1311

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