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downstream to the T7 promotor also results in full – and upon Notes and references
illumination reversible – silencing of transcription, while random
1 C. Brieke, F. Rohrbach, A. Gottschalk, G. Mayer and A. Heckel,
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indicating that the T7 RNAP can efficiently transcribe past
4MNB-modified sites (Fig. S21, ESI†). The fact, that already a
single 4MNB group located in proximity to the promoter either
on coding or noncoding strand (see Fig. S7 and S8, ESI†) aborts
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need for methods that enable manipulation of processes in
living cells, we anticipate a broad range of applications for this
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This work was supported by the Max Planck Society. The
authors thank M. Mu¨ller and S. Fabritz for technical support
and J. Reinstein, A. Meinhart and E. Weinhold for discussions.
C. B. thanks I. Schlichting (MPI-Hd) for her constant support.
Open Access funding provided by the Max Planck Society.
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Conflicts of interest
There are no conflicts to declare.
This journal is ©The Royal Society of Chemistry 2018
Chem. Commun., 2018, 54, 12718--12721 | 12721