Cleavage of oligodeoxyribonucleotides from polymer supports and their rapid deprotection under microwaves

Article abstract of DOI:10.1080/07328319808004711

Novel conditions for the cleavage of oligodeoxynucleotides from polymer supports and their complete deprotection under microwaves have been developed. The oligonucleotides synthesized using phosphoramidite synthons carrying base labile (Pac, Dmf and t-Bpac) and conventional (Bz for A and C and Pac for G) protecting groups for nucleic bases were deprotected using 0.2M sodium hydroxide (MeOH : H₂O :: 1:1, v/v) = Reagent A and 1M sodium hydroxide (MeOH : H₂O :: 1:1, v/v) = Reagent B, respectively under microwaves. The deprotected oligonucleotides were found to be comparable with the corresponding oligonucleotides deprotected under standard conditions (aq. ammonia at 55°C).

Full text of DOI:10.1080/07328319808004711

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Cleavage Of Oligodeoxyribonucleotides
From Polymer Supports And Their Rapid
Deprotection Under Microwaves
a
a
K. C. Gupta & P. Kumar
a
Nucleic Acids Research Laboratory, Centre for Biochemical
Technology , Mall Road, Delhi University Campus, Delhi, 110 007,
India
Published online: 22 Aug 2006.
To cite this article: K. C. Gupta & P. Kumar (1998) Cleavage Of Oligodeoxyribonucleotides From
Polymer Supports And Their Rapid Deprotection Under Microwaves, Nucleosides and Nucleotides,
17:9-11, 1761-1766, DOI: 10.1080/07328319808004711
To link to this article: http://dx.doi.org/10.1080/07328319808004711
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