5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER

Base Information

• Chemical Name: 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER
• CAS No.: 197850-75-2
• Molecular Formula: C35H31NO11
• Molecular Weight: 641.631
• Mol file: 197850-75-2.mol

Synonyms:6-CARBOXYFLUORESCEIN-PIV SE;6-CARBOXYFLUORESCEIN DIPIVALATE-N-HYDROXYSUCCINIMIDE;6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER;5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER;5,6-CARBOXYFLUORESCEIN-PIV SE;6-CARBOXYFLUORESCEIN DIPIVALATE NHS ESTER;6-CARBOXYFLUORESCEIN DIPIVALATE N-HYDROXYSUCCINIMIDE ESTER

Chemical Property of 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER

Chemical Property:

• PSA: 151.81000
• LogP: 5.31630

Purity/Quality:

≥98% ^*data from raw suppliers

6-CarboxyfluoresceinDipivalateN-HydroxysuccinimideEster ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Uses: 6-Carboxyfluorescein Dipivalate N-Hydroxysuccinimide Ester is used as a probe in fluorescence activation-coupled protein labeling method for real-time imaging/measurements of protein dynamics.

Technology Process of 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER

There total 4 articles about 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 92.0%

→ 1-{[(3,6-di-O-pivaloyl)fluorescein-6-yl]oxy}pyrrolidine-2,5-dione (197850-75-2)

Guidance literature:

Reference yield:

1-hydroxy-pyrrolidine-2,5-dione (6066-82-6) + 3',6'-bis(2,2-dimethyl-1-oxopropoxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid (192374-17-7) → 1-{[(3,6-di-O-pivaloyl)fluorescein-6-yl]oxy}pyrrolidine-2,5-dione (197850-75-2)

Guidance literature:

With N-(3-dimethylaminopropyl)-N-ethylcarbodiimide; In various solvent(s);

DOI:10.1021/jo991449h

Reference yield:

2,2-dimethylpropanoic anhydride (1538-75-6) → 1-{[(3,6-di-O-pivaloyl)fluorescein-6-yl]oxy}pyrrolidine-2,5-dione (197850-75-2)

Guidance literature:

Multi-step reaction with 2 steps

1: DIPEA / dimethylformamide; various solvent(s) / 72 h / 20 °C

2: EDC / various solvent(s)

With N-(3-dimethylaminopropyl)-N-ethylcarbodiimide; N-ethyl-N,N-diisopropylamine; In N,N-dimethyl-formamide; 1: Esterification / 2: Esterification;

DOI:10.1021/jo991449h

upstream raw materials:

1-hydroxy-pyrrolidine-2,5-dione

3',6'-bis(2,2-dimethyl-1-oxopropoxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid

2,2-dimethylpropanoic anhydride

6-carboxyfluorescein

Downstream raw materials:

N-(3',6'-dipivaloylfluorescein-6-ylcarbonyl)-3'-amino-3'-deoxythymidine