10.1002/chem.201901151
The study presents the development of a novel genetically encoded fluorescent tag for live cell microscopy, which is based on the fluorogen-activating protein FAST and a new fluorogenic derivative of a GFP-like chromophore with red fluorescence. This tag allows for the reversible binding of the fluorogen to FAST, resulting in a significant increase in red fluorescence (580-650 nm). The researchers found that the new dye can rapidly stain target cellular proteins fused with FAST, wash out quickly, and exhibit higher photostability compared to previously published fluorogen:FAST complexes, making it suitable for use in confocal and widefield microscopy.
10.1016/j.bmc.2008.05.013
The research focuses on the synthesis and evaluation of novel uracil nucleotide derivatives as agonists for the P2Y2 and P2Y6 receptors, which are G protein-coupled receptors activated by nucleotides. The study involves structural modifications of the phosphate, uracil, and ribose moieties of uracil nucleotides to assess their agonist activity at human P2Y2, P2Y4, and P2Y6 receptors. Key modifications include the 2-thio modification, phosphonomethylene bridges for stability, and truncation of dinucleotide agonists. The synthesized compounds were tested for their ability to activate phospholipase C (PLC) in human astrocytoma cells stably expressing the respective P2Y receptors. The experiments utilized various analytical techniques such as NMR, HPLC, and HRMS for compound identification and purity assessment. The main reactants included uracil nucleotides, phosphonic acids, and other chemical modifiers used to synthesize the novel derivatives. The analyses were conducted to determine the EC50 values of the compounds, reflecting their potency in stimulating PLC activity, and to explore structure-activity relationships (SARs).
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