10.1002/chem.201901151
Chemistry - A European Journal
COMMUNICATION
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We compared target staining and the background fluorescence
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intensive green and blue light irradiation (Figure 6).
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Figure 6. Photobleaching behavior of fluorogen:FAST complexes with
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The dye appears to be non-toxic at the 10 μM concentration: the
cells continue to divide in the presence of N871b in the cultural
media at the same rate (see SI part 13) which makes it suitable
for live-cell imaging applications.
To conclude, we report on the development of a new efficient
substrate for the fluorogen-activating FAST protein. The novel
fluorogen N871b forms complex with FAST which is three orders
of magnitude brighter than the free fluorogen and is
characterized by red-shifted emission (580-650 nm). The N871b
instantly stains the target cellular proteins fused with FAST and
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Conflicts of interest
There are no conflicts to declare.
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Acknowledgements
The authors gratefully acknowledge support from the Russian
Science Foundation, grant No. 18-73-10105.
Keywords: fluorescence • chromophore • fluorogen-activating
protein • fluorescent labelling • GFP
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