10.1021/ja0162212
The research focuses on the nucleic acid template-directed assembly of metallosalen-DNA conjugates, aiming to expand the versatility of nucleic acid base pairing for the addressable synthesis of bioconjugates in aqueous solutions. The study demonstrates a unique approach to synthesizing a new class of metal-DNA hybrids, which have potential applications in DNA-organized materials, nucleic acid cleavage and detection strategies, and in vitro evolution of novel ribozymes and deoxyribozymes. The process involves the use of DNA oligonucleotides modified with salicylaldehyde moieties, a complementary nucleic acid template, and the addition of an appropriate metal and diamine to assemble the metallosalen conjugate. Key chemicals used in this process include salicylaldehyde, diamine, metal ions (such as Mn and Ni), ethylenediamine (EN), and manganese(II) acetate (Mn(OAc)2). The conclusions of the research highlight the efficient template-directed synthesis and characterization of metallosalen-DNA conjugates, showcasing their potential for applications in targeted nucleic acid cleavage, biosensors, and catalysis.