DIHYDROFLUORESCEIN DIACETATE

Base Information

• Chemical Name: DIHYDROFLUORESCEIN DIACETATE
• CAS No.: 35340-49-9
• Molecular Formula: C24H18O7
• Molecular Weight: 418.403
• Hs Code.: 29329990
• Mol file: 35340-49-9.mol

Synonyms:Dihydrofluoresceindiacetate;

Chemical Property of DIHYDROFLUORESCEIN DIACETATE

Chemical Property:

• Vapor Pressure: 4.01E-13mmHg at 25°C
• Melting Point: 213-215 °C(lit.)
• Refractive Index: 1.626
• Boiling Point: 554.3 °C at 760 mmHg
• PKA: 3.85±0.36(Predicted)
• Flash Point: 190.3 °C
• PSA: 99.13000
• Density: 1.353g/cm³
• LogP: 4.52130

Purity/Quality:

99.90% ^*data from raw suppliers

Dihydrofluorescein diacetate ^*data from reagent suppliers

Safty Information:

• Safety Statements: 22-24/25

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Uses: Dihydrofluorescein diacetate is a chemical used for intracellular staining. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as 2'',7''-dichlorodihydrofluorescein and Dihydrorhodamine 123. Dihydrofluorescein diacetate is first hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by hydrogen peroxide.

Technology Process of DIHYDROFLUORESCEIN DIACETATE

There total 5 articles about DIHYDROFLUORESCEIN DIACETATE which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 94.0%

dihydrofluorescein (518-44-5) + acetic anhydride (108-24-7) → dihydrofluorescein diacetate (35340-49-9)

Guidance literature:

In pyridine; Ambient temperature;

DOI:10.1002/hlca.19840670136

Reference yield:

O,O'-Dibenzylfluorescein (97744-44-0) → dihydrofluorescein diacetate (35340-49-9)

Guidance literature:

Multi-step reaction with 2 steps

1: H₂ / Pd(OH)2/C / ethanol / 0.5 h / Ambient temperature

2: pyridine / Ambient temperature

With pyridine; hydrogen; palladium dihydroxide; In ethanol;

Reference yield:

fluorescein (2321-07-5,126605-73-0) → dihydrofluorescein diacetate (35340-49-9)

Guidance literature:

Multi-step reaction with 2 steps

1: pyridine

2: acetic acid; zinc / tetrahydrofuran / 0.25 h / 85 °C / Microwave irradiation

With pyridine; acetic acid; zinc; In tetrahydrofuran;

DOI:10.1002/bkcs.12181

upstream raw materials:

dihydrofluorescein

acetic anhydride

O,O'-Dibenzylfluorescein

fluorescein

Downstream raw materials:

erythrosine B

dihydrofluorescein

Refernces

• 1、 Le, Hoa Thi,Jo, Hye-Ryeong,Oh, Se-Yun,Jung, Jinwook,Kim, Young Gi,Kang, Chulhun,Kim, Tae Woo. "Endoplasmic Reticulum Targeting Reactive Oxygen Species Sensor Based on Dihydrofluorescein: Application of Endoplasmic Reticulum Stress". . p. 279 - 285. Retrieved 2020/12/21.
• 2、 Tadic, Dragana,Brossi, Arnold. "CHIRAL PRODYES. ETHERS AND ESTERS OF DIHYDROFLUORESCEIN, PART 1: DIBENZYLDIHYDROFLUORESCEIN (DBDF) A NEW REAGENT". . p. 1975 - 1982. Retrieved 2007/10/02.