COELENTERAZINE 400 A

Base Information

• Chemical Name: COELENTERAZINE 400 A
• CAS No.: 70217-82-2
• Molecular Formula: C26H21N3O
• Molecular Weight: 391.472
• Mol file: 70217-82-2.mol

Synonyms:2,8-DIBENZYL-6-PHENYL-IMIDAZO[1,2A]PYRAZIN-3-(7H)-ONE;BISDEOXYCOELENTERAZINE;COELENTERAZINE 400 A;DI-DEHYDRO COELENTERAZINE;DEEPBLUEC(TM)

Chemical Property of COELENTERAZINE 400 A

Chemical Property:

• Vapor Pressure: 3.33E-12mmHg at 25°C
• Refractive Index: 1.661
• Boiling Point: 551.4°C at 760 mmHg
• Flash Point: 287.3°C
• PSA: 50.16000
• Density: 1.2g/cm³
• LogP: 4.87120

Purity/Quality:

98%,99%, ^*data from raw suppliers

Coelenterazine400a ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Uses: Coelenterazine 400a is a chromophore that produces the chemiluminescence displayed by the Aequorin protein in the presence of Ca2+.

Technology Process of COELENTERAZINE 400 A

There total 16 articles about COELENTERAZINE 400 A which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 80.0%

benzylglyoxal (56485-04-2) + 2-amino-3-benzyl-5-phenyl-pyrazine (70217-86-6) → 2-benzyl-8-benzyl-6-(phenyl)imidazo[1,2-a]pyrazin-3(7H)-one (70217-82-2)

Guidance literature:

benzylglyoxal; 2-amino-3-benzyl-5-phenyl-pyrazine; In ethanol; for 0.333333h; Inert atmosphere;

With hydrogenchloride; In ethanol; water; for 18h; Reflux;

Reference yield: 71.7%

1,1-diethoxy-3-phenylpropane-2-one (19256-31-6) + 2-amino-3-benzyl-5-phenyl-pyrazine (70217-86-6) → 2-benzyl-8-benzyl-6-(phenyl)imidazo[1,2-a]pyrazin-3(7H)-one (70217-82-2)

Guidance literature:

With hydrogenchloride; In ethanol; Heating;

DOI:10.1021/ja073936h

Reference yield: 30.0%

ethyl (3-benzyl-5-phenylpyrazin-2-yl)phenylalaninate → 2-benzyl-8-benzyl-6-(phenyl)imidazo[1,2-a]pyrazin-3(7H)-one (70217-82-2)

Guidance literature:

With hydrogenchloride; In ethanol; at 0 - 90 ℃; Inert atmosphere; Sealed tube;

upstream raw materials:

1,1-diethoxy-3-phenylpropane-2-one

2-amino-3-benzyl-5-phenyl-pyrazine

tributylphenylstannane

benzylglyoxal

Downstream raw materials:

2,8-dibenzyl-3-methoxy-6-phenyl-imidazo[1,2-a]pyrazine