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Rhodamine B
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Name

Rhodamine B

 EINECS 201-383-9
CAS No. 81-88-9 Density 0.79 g/mL at 20 °C
PSA 56.69000 LogP 2.56500
Solubility soluble in water Melting Point 210-211 (dec.)(lit.)
Formula C28H31N2O3.Cl Boiling Point N/A
Molecular Weight 479.019 Flash Point 12°C
Transport Information UN 1219 3/PG 2 Appearance red/brown or green crystals
Safety 7-16-24/25-26-36/37/39-39-36-22 Risk Codes 22-41-68-67-36-11-40-20/21/22
Molecular Structure Molecular Structure of 81-88-9 (Rhodamine B) Hazard Symbols HarmfulXn,IrritantXi,FlammableF
Synonyms

Rhodamine BXL;Rhodamine BXP;Rhodamine Lake Red B;Rhodamine O;Rhodamine S (Russian);Rhodamine, tetraethyl-;Sicilian Cerise Toner A 7127;Sumiplast Pink B;Symulex Magenta F;Symulex Rhodamine B Toner F;TakaokaRhodamine B;Calcozine Rhodamine BXP;Cerise Toner X1127;Chromatint Rhodamine B;D and C Red No. 19;C RedNo. 19;Diabasic Rhodamine B;Eriosin Rhodamine B;FD And C Red No. 19;Flexo Red 540;Hexacol Rhodamine BExtra;Ikada Rhodamine B;Japan Red No. 213;OP 312;Red No. 213;Rhodamine B;Rhodamine B Extra;Rhodamine B Extra M 310;Rhodamine BA;Rhodamine BAExport;11411 Red;ADC Rhodamine B;AizenRhodamine BH;Akiriku Rhodamine B;BasicRose Extract;Basonyl Red 540;Basonyl Red 545FL;C.I. Basic Violet 10;Calcozine Red BX;

Article Data 19

Rhodamine B Synthetic route

C34H34N5O2(1+)*Cl(1-)

A

95-14-7

1,2,3-Benzotriazole

B

81-88-9

rhodamine B

Conditions
ConditionsYield
With water In acetonitrile at 20℃; for 1h;
1167601-09-3

3',6'-bis(diethylamino)-2-(2-hydroxyethylamino)spiro[iso-indoline-1,9'-xanthen]-3-one

81-88-9

rhodamine B

Conditions
ConditionsYield
With Cu+
760154-10-7

rhodamine B hydrazide

81-88-9

rhodamine B

Conditions
ConditionsYield
With mercury (II) perchlorate trihydrate; water In ethanol at 20℃; pH=7.0;
74317-53-6

rhodamine B hydrazide

81-88-9

rhodamine B

Conditions
ConditionsYield
Multi-step reaction with 3 steps
1: methanol / 12 h / 65 °C / Inert atmosphere
2: mercury (II) perchlorate trihydrate / ethanol; water / 20 °C / pH 7.0
3: water; mercury (II) perchlorate trihydrate / ethanol / 20 °C / pH 7.0
View Scheme
Multi-step reaction with 2 steps
1: ethanol / 6 h / Reflux
2: mercuric(II) nitrate monohydrate / ethanol / 0.08 h
View Scheme
4344-42-7

rhodamine B

81-88-9

rhodamine B

Conditions
ConditionsYield
With trichlorophosphate In 1,2-dichloro-ethane for 2h; Reflux;
1374146-64-1

C39H39N5O4

A

5326-89-6

quinolin-8-yloxy-acetic acid

B

81-88-9

rhodamine B

Conditions
ConditionsYield
With water; hypochlorite In acetonitrile at 25℃;

C39H38N4O2

81-88-9

rhodamine B

Conditions
ConditionsYield
With mercuric(II) nitrate monohydrate In ethanol for 0.0833333h; Mechanism;
85-44-9

phthalic anhydride

91-68-9

3-diethylaminophenol

81-88-9

rhodamine B

Conditions
ConditionsYield
Stage #1: phthalic anhydride; 3-diethylaminophenol In 1,2-dichloro-benzene at 175℃; for 13h;
Stage #2: With water; sodium hydroxide at 25℃; for 0.5h;
Stage #3: With hydrogenchloride; sodium chloride In water at 80℃; for 1h;		35 g
With sulfuric acid at 150℃; for 2h;

C36H39N5O4

81-88-9

rhodamine B

Conditions
ConditionsYield
With copper(II) ion In water; acetonitrile Reagent/catalyst;
509-34-2

rhodamine B

81-88-9

rhodamine B

Conditions
ConditionsYield
With trichlorophosphate In 1,2-dichloro-ethane for 3h; Reflux;

Rhodamine B Chemical Properties

Molecular Structure:

Molecular Formula: C28H31ClN2O3
Molecular Weight: 479.0103 
IUPAC: [9-(2-Carboxyphenyl)-6-(diethylamino)xanthen-3-ylidene]-diethylazanium chloride
Synonyms of Rhodamine B (CAS NO.81-88-9): 9-(2-Carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride ; Ammonium, (9-(o-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene)diethyl-, chloride ; C.I. Basic Violet 10 ; Ethanaminium, N-(9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene)-N-ethyl-, chloride (9CI) ; Xanthylium, 9-(2-carboxyphenyl)-3,6-bis(diethylamino)-, chloride ; Xanthylium, 9-(2-carboxyphenyl)-3,6-bis(diethylamino)-, chloride (1:1) ; C.I. Food Red 15 ; D&C Red No. 19
CAS NO: 81-88-9
Classification Code: Agricultural Chemical ; Experimental pesticide ; Mutation data ; Reproductive Effect ; Tumor data ; Unspecified / Unclassified pesticide
Melting point: 210-211 °C
Density: 0.79 g/mL at 20 °C
Flash Point:  12 °C
Storage temp: Store at RT.
Colour Index: 45170
Water Solubility: SOLUBLE
BRN: 4119648
Stability: Stable. Incompatible with strong oxidizing agents.

Rhodamine B Uses

  Rhodamine B (CAS NO.81-88-9) is used as dye, esp for paper, as reagent for antimony, bismuth, cobalt, niobium, gold, manganese, mercury, molybdenum, tantalum, thallium, tungsten, as biological stain.

Rhodamine B Toxicity Data With Reference

Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
mouse LD50 intraperitoneal 144mg/kg (144mg/kg)   "Recent Advances of Chemistry and Molecular Biology in Cancer Research, Insternational Symposium on Recent Advances of Chemistry and Molecular Biology in Cancer Research, Held in Beijing/China, July 23rd-26th, 1991" Dai Qianhuan, Margaret-Ann Armour and Zheng Qingying eds. Science Press Vol. -, Pg. 281, 1993.
mouse LD50 oral 887mg/kg (887mg/kg)   Toxicological European Research. Vol. 1, Pg. 23, 1978.
mouse LD50 subcutaneous 180mg/kg (180mg/kg)   Osaka-furitsu Koshu Eisei Kenkyusho Kenkyu Hokoku, Koshu Eisei Hen. Research Reports of the Oaka Prefectural Institute of Public Health, Public Health Section. Vol. (3), Pg. 185, 1953.
rat LD50 intraperitoneal 112mg/kg (112mg/kg)   Toxicological European Research. Vol. 1, Pg. 23, 1978.
rat LD50 intravenous 89mg/kg (89mg/kg)   Toxicology and Applied Pharmacology. Vol. 3, Pg. 696, 1961.
rat LDLo oral 500mg/kg (500mg/kg)   National Academy of Sciences, National Research Council, Chemical-Biological Coordination Center, Review. Vol. 5, Pg. 8, 1953.

Rhodamine B Safety Profile

Hazard Codes of Rhodamine B (CAS NO.81-88-9): HarmfulXn,Xi,FlammableF
Risk Statements: 22-41-68-67-36-11
R22: Harmful if swallowed. 
R41: Risk of serious damage to the eyes. 
R68: Possible risk of irreversible effects. 
R67: Vapours may cause drowsiness and dizziness. 
R36: Irritating to eyes. 
R11: Highly flammable.
Safety Statements: 7-16-24/25-26-36/37/39 
S7: Keep container tightly closed. 
S16: Keep away from sources of ignition. 
S24/25: Avoid contact with skin and eyes. 
S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. 
S36/37/39: Wear suitable protective clothing, gloves and eye/face protection.
WGK Germany: 2
 

Rhodamine B Specification

Stability: Stable at room temperature in closed containers under normal storage and handling conditions.
Storage：Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances
Handling：Wash thoroughly after handling. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Use with adequate ventilation. Wash clothing before reuse.
First Aid Measures:
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid.
Inhalation: Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
Skin: Get medical aid. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.
 

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