To use this system for quantitative measurements, you may need to create a calibration curve relating the change in the fluorescence intensity ratio to the concentration of Cu2+. This allows for the determination of Cu2+ concentration in a given sample.
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C14H9NHSO2C6H4CH3
C28H22N8O6
tris[2-(5-nitrosalicylideneamino)ethyl]amine
