Some ligation methods, such as NCL, involve the use of cysteine residues for ligation. To achieve traceless purification, you may need to desulfurize the product by selectively removing thioether (sulfur) groups without affecting other functional groups.
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2-azido-1-(2-nitrophenyl)ethyl (2,5-dioxopyrrolidin-1-yl)carbonate
O-ethyl ethylphosphorochloridothioate
