After synthesizing the compound, it's crucial to evaluate its inhibitory activity against thymidylate synthase. This typically involves biochemical assays to determine the compound's potency as an antifolate inhibitor.
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2-[4-({[2-(2,2-dimethyl-propionylamino)-6-methyl-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-ylmethyl]-amino}-methyl)-benzoylamino]-pentanedioic acid
