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9008-97-3

Basic Information
CAS No.: 9008-97-3
Name: Phytohemagglutinins
Molecular Structure:
Molecular Structure of 9008-97-3 (Phytohemagglutinins)
Formula: N/A
Molecular Weight: 0
Synonyms: Agglutininsand Lectins, phytohemagglutinins; Hemagglutinins, phyto-; Agglutinins andLectins, phytohem-; Agglutinins and lectins, phytohemagglutinin; Agglutinins,phytohemagglutinin; Agglutinins, phytohemagglutinins; Hemagglutinins,phytohemagglutinins; Lectins, phytohemagglutinin; Lectins, phytohemagglutinins;PHA; PHA (phytohemagglutinins); Phytohem- agglutinins and Lectins;Phytohemagglutinin; Phytohemagglutinins agglutinins and Lectins; Phytolectin
EINECS: 291-083-4
Density: N/A
Melting Point: N/A
Boiling Point: N/A
Flash Point: N/A
Solubility: PBS: 2 mg/mL
Appearance: N/A
Hazard Symbols: N/A
Risk Codes: N/A
Safety: 22-24/25
Transport Information: N/A
PSA: 0.00000
LogP: 0.00000
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Chemistry

Product Name: Phytohaemagglutinin
Synonyms: Fitohemaglutynina ; Phytohaemagglutinin ; Phytohemagglutinin ; Phytohemaglutinins ; Pigeonpeaphytohemagglutinin ; Leucoagglutinin (pha-l) ; Red kidney bean lectin ; Phaseolus vulgaris agglutinin
CAS NO: 9008-97-3
EINECS: 291-083-4
Storage temp: 2-8°C
Solubility : PBS: 2 mg/mL
Form :salt-free, lyophilized powder

Uses

Phytohaemagglutinin (CAS NO.9008-97-3) has a number of physiological effects and is used in medical research. In high doses it is a toxin.
1.Phytohaemagglutinin (CAS NO.9008-97-3) has a number of effects on cell metabolism: it induces mitosis, and affects the cell membrane in regard to transport and permeability to proteins. It agglutinates most mammalian red blood cell types.
2.As a toxin Phytohaemagglutinin (CAS NO.9008-97-3) can cause poisoning in monogastric animals, such as humans, through the consumption of raw or improperly prepared kidney beans.
3. Medically Phytohaemagglutinin (CAS NO.9008-97-3) is used as a mitogen to trigger cell division in T-lymphocytes, and to activate latent HIV-1 from human peripheral lymphocytes. PHA-L is used by neuroscientists to trace the path of efferent axons of neurons. This usage is called the anterograde labeling method.
Lymphocytes cultured with Phytohaemagglutinin can be used for karyotype analysis.

Production

Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants, especially beans.PHA actually consists of two closely related proteins, called Leucoagglutinin (PHA-L) and PHA-E.It is found in the highest concentrations in uncooked red kidney beans and white kidney beans (also known as cannellini), and it is also found in lower quantities in many other types of green beans and other common beans (Phaseolus vulgaris), as well as broad beans (Vicia faba) such as fava beans.

Toxicity Data With Reference

Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
rat LDLo intraperitoneal 1470mg/kg (1470mg/kg) BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY)

BEHAVIORAL: FOOD INTAKE (ANIMAL)

LUNGS, THORAX, OR RESPIRATION: DYSPNEA
Toxicology and Applied Pharmacology. Vol. 40, Pg. 217, 1977.

Safety Profile

An experimental teratogen. Experimental reproductive effects. Mutation data reported.
Safety Statements 22-24/25
S22:Do not breathe dust. 
S24/25:Avoid contact with skin and eyes.
RTECS TJ3470000
F 10-21

Specification

Poisoning can be induced from as few as five raw beans and symptoms occur within three hours, beginning with nausea then vomiting which can be severe and sustained (profuse), then diarrhea. Recovery occurs within four or five hours of onset, usually without the need for any medical intervention.