10017-07-9Relevant articles and documents
Synthesis of (R)-cyanohydrins by crude (R)-oxynitrilase-catalyzed reactions in micro-aqueous medium
Han, Shiqing,Lin, Guoqiang,Li, Zuyi
, p. 1835 - 1838 (1998)
In diisopropyl ether or ethyl acetate under micro-aqueous conditions, the enantioselective synthesis of (R)-cyanohydrins from aldehydes and methyl ketones was studied using crude (R)-oxynitrilase prepared from almonds. This reaction system performed well
Hydroxynitrile lyase from Arabidopsis thaliana: Identification of reaction parameters for enantiopure cyanohydrin synthesis by pure and immobilized catalyst
Okrob, Daniel,Paravidino, Monica,Orru, Romano V. A.,Wiechert, Wolfgang,Hanefeld, Ulf,Pohl, Martina
, p. 2399 - 2408 (2011)
The (R)-selective hydroxynitrile lyase from Arabidopsis thaliana (AtHNL) is a promising biocatalyst for the synthesis of a broad range of chiral cyanohydrins. However, the enantiomeric excess of the reaction is strongly compromised by a non-catalyzed side
Immobilized hydroxynitrile lyases for enantioselective synthesis of cyanohydrins: Sol-gels and cross-linked enzyme aggregates
Cabirol, Fabien L.,Hanefeld, Ulf,Sheldon, Roger A.
, p. 1645 - 1654 (2006)
The hydroxynitrile lyases (HNLs) from Prunus amygdalus (paHNL), Manihot esculenta (MeHNL), and Hevea brasiliensis (HbHNL) were successfully immobilized in sol-gels. The cross-linked enzyme aggregate (CLEA) of HbHNL was also prepared. These immobilized enzymes and the commercial PaHNL- and MeHNL-CLEAs were employed for the enantioselective synthesis of cyanohydrins. The sol-gels were highly efficient at low catalyst loading and particularly stable towards the organic solvent (diisopropyl ether) and substrate/product deactivation. The stabilization effect was inconsistent for CLEAs of different HNLs and significant deactivation of PaHNL- and HbHNL-CLEAs in diisopropyl ether was observed. In contrast commercial MeHNL-CLEA proved to be a remarkably robust and efficient biocatalyst in diisopropyl ether.
A practical high through-put continuous process for the synthesis of chiral cyanohydrins
Chen, Peiran,Han, Shiqing,Lin, Guoqiang,Li, Zuyi
, p. 8251 - 8253 (2002)
A practical high through-put continuous process for the synthesis of chiral cyanohydrins is reported. Pretreated almond meal (or other solid raw enzyme sources) was loaded in a column to form a reactor, to which were attached a supplying system to deliver
Evaluation of guanabana (Annona muricata) seed meal as a source of (S)-oxynitrilase
Solis, Aida,Luna, Hector,Perez, Herminia I.,Manjarrez, Norberto
, p. 2351 - 2353 (2003)
Guanabana seed meal is a source of (S)-oxynitrilase which biocatalyzes the enantioselective addition of HCN to aromatic, heteroaromatic and α,β-unsaturated aldehydes to produce cyanohydrins.
Fusion of a Coiled-Coil Domain Facilitates the High-Level Production of Catalytically Active Enzyme Inclusion Bodies
Diener, Martin,Kopka, Benita,Pohl, Martina,Jaeger, Karl-Erich,Krauss, Ulrich
, p. 142 - 152 (2016)
The increasing number of biocatalytic reactions implemented in chemical synthesis routes raises the urgent need for large amounts of enzymes. Hence, new generic methods are required for their simple and cost-efficient production. Here, we describe a gener
Chemoenzymatic synthesis of Fmoc-protected (2S,3S)-2-hydroxy-3-amino acids and their application in the synthesis of an α-hydroxylated β-hexapeptide
Tromp, Reynier A.,Van der Hoeven, Michael,Amore, Alessia,Brussee, Johannes,Overhand, Mark,Van der Marel, Gijs A.,Van der Gen, Arne
, p. 1109 - 1112 (2001)
A chemoenzymatic and stereoselective synthesis of Fmoc-protected (2S,3S)-2-hydroxy-3-amino acids from 2-furaldehyde is described as well as their application, without prior hydroxyl protection, in the solid-phase synthesis of a novel completely α-hydroxylated β-hexapeptide.
BINOLAM, a recoverable chiral ligand for bifunctional enantioselective catalysis: The asymmetric synthesis of cyanohydrins
Casas, Jesus,Najera, Carmen,Sansano, Jose M.,Saa, Jose M.
, p. 2589 - 2592 (2002)
(Matrix presented) A new bifunctional catalytic system based on a monometallic aluminum complex is used for the efficient enantioselective cyanation of aldehydes. The ligand (S)- or (R)-2,2′-bis(diethylaminomethyl)-substituted binaphthol (BINOLAM) used is
A study of asymmetric hydrocyanation of heteroaryl carboxaldehydes catalyzed by (R)-oxynitrilase under micro-aqueous conditions
Chen, Peiran,Han, Shiqing,Lin, Guoqiang,Huang, Hao,Li, Zuyi
, p. 3273 - 3279 (2001)
A number of new optically active heteroaryl cyanohydrins have been prepared by hydrocyanation under micro-aqueous conditions catalyzed by almond meal (containing (R)-oxynitrilase). Substituent effects on the reaction are discussed. This micro-aqueous meth
Enzymatic Synthesis of (R)-Cyanohydrins by Three (R)-Oxynitrilase Sources in Micro-aqueous Organic Medium
Lin, Guoqiang,Han, Shiqing,Li, Zuyi
, p. 3531 - 3540 (1999)
The enantioselective synthesis of optically active (R)-cyanohydrins generated from several aromatic, heteroaromatic and aliphatic aldehydes and methyl ketones was carried out using almond, peach or loquat meal as (R)-oxynitrilase sources in diisopropylether under micro-aqueous conditions The micro-aqueous reaction system, which is superior to the conventionally used water-organic biphase reaction system, performed well over the temperature range of 4 deg C to 30 deg C.
