103251-34-9

Upstream product

• 50-89-5 thymidine 
• 76-83-5 trityl chloride 
• 7791-71-1 5'-O-tritylthymidine 
• 42214-24-4 1-(2-deoxy-3-O-methanesulfonyl-5-O-trityl-β-D-ribopentofuranosyl)thymine 
• 524-38-9 N-hydroxyphthalimide 
• 55612-11-8 5'-O-trityldeoxyxylothymidine 

Downstream Products

• 132497-10-0 C₂₉H₂₉N₃O₅ 
• 157723-20-1 C₅₇H₆₁N₅O₁₂Si 
• 157723-19-8 1-{(2R,4S,5R)-4-Aminooxy-5-[bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-tetrahydro-furan-2-yl}-5-methyl-1H-pyrimidine-2,4-dione 

Relevant academic research and scientific papers

Synthesis of an oxyamide linked nucleotide dimer and incorporation into antisense oligonucleotide sequences

Two derivatives of thymidine, a 5'-protected 3'-hydroxylamine and 5'-carboxy-3'-protected compound, were prepared and coupled to form an oxyamide-linked dinucleotide analogue; this dimer was incorporated into an oligodeoxynucleotide then shown to anneal t

Optimized synthesis of 3′-O-aminothymidine and evaluation of its oxime derivative as an anti-HIV agent

The synthesis and isolation of 3′-O-aminothymidine oximes have been optimized. Synthesized compounds were characterized by NMR and UV spectral and analytical data. A mixture of previously not reported syn and anti isomers of acetaldoximes was assessed for anti-HIV activity and the prevention of syncytia formation caused by HIV-1 infection.

Method for sequencing DNA and RNA by synthesis

This invention relates to the field of nucleic acid chemistry, more specifically to the field of compositions and processes that are nucleic acid analogs. More specifically, this invention relates to compositions that allow the sequencing of oligonucleotides by synthesis, and processes for sequencing by synthesis that exploit these compositions. Most specifically, the instant invention discloses compositions of matter that are 5′-triphosphates of ribo- and 2′-deoxyribonucleosides wherein the 3′-OH group is replaced by a 3′-ONHR group in the alpha configuration, wherein R is either a H or CH3 group. Also disclosed are these triphosphates where the nucleobase carries, via a linker, a reporter groups, such as a fluorescent species that can be used in single- or multi-copy DNA sequencing, or a tag that can be visualized by ultramicroscopy. Also disclosed are processes that use these compositions to do sequencing by synthesis.