10602-14-9Relevant academic research and scientific papers
A novel method for the synthesis of acetyl phosphate
Devedjiev, Ivan
, p. 1785 - 1787 (2006)
A facile method for the synthesis of acetyl phosphate by a reaction of 2-hydroxypropylphosphate with acetic acid is described. Copyright Taylor & Francis Group, LLC.
Regioselective phosphorylation of carbohydrates and various alcohols by bacterial acid phosphatases; probing the substrate specificity of the enzyme from Shigella flexneri
Van Herk, Teunie,Hartog, Aloysius F.,Van Der Burg, Alida M.,Wever, Ron
, p. 1155 - 1162 (2007/10/03)
Bacterial non-specific acid phosphatases normally catalyze the dephosphorylation of a variety of substrates. As shown previously the enzymes from Shigella flexneri and Salmonella enterica are also able to catalyze the phosphorylation of inosine to inosine monophosphate and D-glucose to D-glucose 6-phosphate (D-G6P) using cheap pyrophosphate as the phosphate donor. After optimization high yields (95%) are achieved in the latter reaction and we show here that it is possible to use these enzymes in a preparative manner. This prompted us to investigate by using 31P NMR and HPLC also the phosphorylation of a broad range of carbohydrates and alcohols. Many cyclic carbohydrates are phosphorylated in a regioselective manner. Non-cyclic carbohydrates are phosphorylated as well. Phosphorylation of linear alcohols, cyclic and aromatic alcohols is also possible. In all cases the acid phosphatase from Shigella prefers a primary alcohol function above a secondary one. We conclude that these enzymes are an attractive alternative to existing chemical and enzymatic methods in the phosphorylation of a broad range of compounds.
Metal ion induced allosteric transition in the catalytic activity of an artificial phosphodiesterase
Takebayashi, Shinji,Ikeda, Masato,Takeuchi, Masayuki,Shinkai, Seiji
, p. 420 - 421 (2007/10/03)
An artificial phosphodiesterase (1) bearing two kinds of metal binding sites, a catalytic site and a regulatory bipyridine site showed a unique allosteric transition in the catalytic activity against the metal concentration.
Plasmid relaxation induced by copper metalated diglycine conjugates under heterogeneous reaction conditions
Madhavaiah,Verma, Sandeep
, p. 923 - 926 (2007/10/03)
This paper reports synthesis and plasmid modification activities of a new class of insoluble copper-metalated diglycine conjugates, containing aliphatic linkers of varying length. Besides providing significant rate enhancement for model phosphate ester cl
A Mechanistic Characterization of the Spontaneous Ring Opening Process of Epoxides In Aqueous Solution: Kinetic and Product Studies
Pocker, Y.,Ronald, B. P.,Anderson, K. W.
, p. 6492 - 6497 (2007/10/02)
A careful determination of the extent of rearrangement during epoxide hydration was carried out in conjunction with nuclear magnetic resonance kinetic studies for both propylene and isobutylene oxides.The complete pH-rate profile was mapped at ionic strength 2.0, and solvent kinetic isotope effects were determined for several limbs.Spectrophotometric analysis of the quantity of aldehyde formed by rearrangement in both the acidic and spontaneous limbs reveals that ionic strength has a profound effect.At ionic strength zero, more rearrangement product is generated in the acidic limb, whereas at ionic strength 2.0, larger amounts of aldehyde are formed in both regions.These results support the conclusion that a different carbocationic intermediate exists in the reaction mechanism for the acidic and spontaneous regions.The experimental observations of the spontaneous region can be explained by the existence of zwitterionic intermediate III.Observed solvent kinetic isotope effects, kH3O+/kD3O+, kHspon/kDspon, of 0.33+/-0.02, 1.78+/-0.25 and 0.35+/-0.03, 1.48+/-0.27 for propylene and isobutylene oxides, respectively, were dissected into components on the basis of classical product studies.At high ionic strength, isolation of compound I, an isobutyraldehyde isobutylene glycol acetal, supports the existence of intermediate III.Apparent partitioning ratios for both the β-hydroxy carbocation, II, and the zwitterion, III, are examined and contrasted.Detailed mechanistic proposals are advanced, and their congruence with a variety of observations described previously in this journal is outlined.
