110764-74-4

Usage

Uses

Used in Pharmaceutical Industry:
Cytidine, N-benzoyl-5'-O-[bis(4-Methoxyphenyl)phenylMethyl]-2'-O-Methylis used as a key intermediate in the synthesis of fluorocarbon oligonucleotide conjugates for the delivery of nucleic acids into human cells. This allows for targeted gene therapy and the development of novel treatments for various genetic disorders and diseases.
Used in Biotechnology Industry:
Cytidine, N-benzoyl-5'-O-[bis(4-Methoxyphenyl)phenylMethyl]-2'-O-Methylis used as a building block in the preparation of RNA and RNA fragments, such as in the synthesis of capped RNA. This enables the development of new RNA-based therapeutics and diagnostic tools, as well as the study of RNA structure and function.
Used in Research and Development:
Cytidine, N-benzoyl-5'-O-[bis(4-Methoxyphenyl)phenylMethyl]-2'-O-Methylis used as a research compound for studying the properties and mechanisms of nucleosides and their role in various biological processes. This contributes to the advancement of knowledge in the fields of molecular biology, genetics, and biochemistry.

Upstream product

• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 52571-45-6 2'-O-methyl-N⁴-benzoylcytidine 
• 81246-76-6 4-N-benzoyl-1-(5-O-dimethoxytrityl-β-D-ribofuranosyl)cytosine 
• 74-88-4 methyl iodide 
• 186581-53-3 diazomethane 
• 13089-48-0 N₄-benzoylcytidine 
• 69304-43-4 1-[3,5-O-(1,1,3,3-tetraisopropyldisiloxane-1,3-diyl)-β-D-ribofuranosyl]-N⁴-benzoylcytosine 
• 2140-72-9 2'-O-methylcytidine 
• 93-97-0 benzoic acid anhydride 

Downstream Products

• 110764-78-8 N4-Benzoyl-5'-O-(4,4'-dimethoxytriphenylmethyl)-2'-O-methyl cytidine-3'-O-(β-cyanoethyl N,N-diisopropylphosphoramidite) 
• 118380-94-2 C₄₁H₄₆N₅O₁₄P*H₃N 

Relevant academic research and scientific papers

SYNTHESIS OF OLIGONUCLEOTIDES AND RELATED COMPOUNDS

Methods of synthesizing oligonucleotides via new intermediates on a cleavable support having an azidomethyl moiety are disclosed. The method comprises multiple reaction cycles, each of which comprises sequential coupling a nucleoside or oligonucleotide su

Process for the preparation of 2'-O-alkyl purine phosphoramidites

2'-O-alkylated guanosine, uridine, cytidine, and 2,6-diaminopurine 3'-O-phosphoramidites are prepared by alkylating nucleoside precursors, adding suitable blocking groups and phosphitylating. Alkylation is effected on 2,6-diamino-9-(β-D-ribofuranosyl)purine followed by deamination to prepare guanosine 2'-O-alkylated 3'-O-phosphormidites. Alkylation is effected on a dialkyl stannylene derivative of uridine to prepare uridine 2'-O-alkylated 3'-O-phosphormidites. Alkylation is effected directly on cytidine to prepare cytidine 2'-O-alkylated 3'-O-phosphormidites. Alkylation is effected directly on 2,6-diaminopurine to prepare 2,6-diaminopurine 2'-O-alkylated 3'-O-phosphormidites.

Oligo(2′-O-methylribonucleotides) and their derivatives. II. Synthesis and properties of oligo(2′-O-methylribonucleotides) modified with N-(2-hydroxyethyl)phenazinium and steroid groups at the 5′-terminus

Oligo(2′-O-methylribonucleotides) modified at the 5′-terminus with a steroid (cholesterol or testosterone) or polycyclic aromatic dye N-(2-hydroxyethyl)phenazinium residue were synthesized. It was shown that the introduction of an N-(2-hydroxyethyl)phenazinium moiety into octa(2′-O-methylribonucleotide) increased the melting temperature of the duplex with the d-target by 9°C. The steroid residue, which was attached to the 5′-position of deca(2′-O-methyluridylate) via a phosphodiester linkage, enhanced the stability of the steroid conjugate complexes with d(pA)16 and (pA)16; this effect was stronger with the cholesterol derivative (ΔTm 5 and 8°C, respectively) than with the testosterone derivative(ΔTm 1 and 4°C).

Simplified synthesis of 2′ -O-alkyl ribopyrimidines

Direct 2′-hydroxyl alkylation (R = methyl, ethyl, propyl, pentyl or allyl) of 5′-dimethoxytrityl-N-(t-butylphenoxyacetyl or benzoyl)-cytidine 1 was achieved by reaction in the presence of silver I oxide and a catalytic amount of pyridine with excess alkyl

REGIOSPECIFIC CLEAVAGE OF RNA BY RNASE H FROM E. coli IN THE PRESENCE OF A COMPLEMENTARY OLIGONUCLEOTIDE WITH INSERTED ALTERNATING 2'-O-METHYLCYTIDINE RESIDUES

Undecanucleotide dA-dC-dC-dA-Cm-Cm-dG-Cm-dG-Cm-dT (Cm = 2'-O-methylcytidine) was prepared and used as a probe for splitting of 5S rRNA fragment by RNase H (E. coli).It was found that substitution of 2'-deoxycytidine of d(ACCACCGCGCT) by 2'-O-methylcytidin

SYNTHESIS OF N,O-PROTECTED DERIVATIVES OF 2'-O-METHYLCYTIDINE AND OF 2'-O-METHYL- AND N1-METHYLGUANOSINES

Using selective 3',5'-O-tetraisopropyldisiloxane protection, N-acyl-2'-O-methyl derivatives of cytidine and guanosine and of 2'-O-tetrahydropyranyl-N1-methyl-guanosine have been synthesized.It has been shown that the spatial screening of the am