1200461-32-0Relevant articles and documents
Nucleotides and nucleosides and methods for their use in DNA sequencing
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, (2015/12/18)
The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.
Stereochemistry of benzylic carbon substitution coupled with ring modification of 2-nitrobenzyl groups as key determinants for fast-cleaving reversible terminators
Stupi, Brian P.,Li, Hong,Wang, Jinchun,Wu, Weidong,Morris, Sidney E.,Litosh, Vladislav A.,Muniz, Jesse,Hersh, Megan N.,Metzker, Michael L.
, p. 1724 - 1727 (2012/05/05)
A complete set of 2-nitrobenzyl-modified reversible terminators have been developed, which upon exposure to UV light generate natural hydroxymethyl nucleotides (see scheme). The combination of a stereospecific (S)-tert-butyl group (R) at the benzylic carb