1241390-31-7Relevant academic research and scientific papers
Red-Shifted Substrates for FAST Fluorogen-Activating Protein Based on the GFP-Like Chromophores
Povarova, Natalia V.,Zaitseva, Snizhana O.,Baleeva, Nadezhda S.,Smirnov, Alexander Yu.,Myasnyanko, Ivan N.,Zagudaylova, Marina B.,Bozhanova, Nina G.,Gorbachev, Dmitriy A.,Malyshevskaya, Kseniya K.,Gavrikov, Alexey S.,Mishin, Alexander S.,Baranov, Mikhail S.
supporting information, p. 9592 - 9596 (2019/07/09)
A genetically encoded fluorescent tag for live cell microscopy is presented. This tag is composed of previously published fluorogen-activating protein FAST and a novel fluorogenic derivative of green fluorescent protein (GFP)-like chromophore with red fluorescence. The reversible binding of the novel fluorogen and FAST is accompanied by three orders of magnitude increase in red fluorescence (580–650 nm). The proposed dye instantly stains target cellular proteins fused with FAST, washes out in a minute timescale, and exhibits higher photostability of the fluorescence signal in confocal and widefield microscopy, in contrast with previously published fluorogen:FAST complexes.
Plug-and-play fluorophores extend the spectral properties of spinach
Song, Wenjiao,Strack, Rita L.,Svensen, Nina,Jaffrey, Samie R.
, p. 1198 - 1201 (2014/02/14)
Spinach and Spinach2 are RNA aptamers that can be used for the genetic encoding of fluorescent RNA. Spinach2 binds and activates the fluorescence of (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one (DFHBI), allowing the dynamic localizations of Spinach2-tagged RNAs to be imaged in live cells. The spectral properties of Spinach2 are limited by DFHBI, which produces fluorescence that is bluish-green and is not optimized for filters commonly used in fluorescence microscopes. Here we characterize the structural features that are required for fluorophore binding to Spinach2 and describe novel fluorophores that bind and are switched to a fluorescent state by Spinach2. These diverse Spinach2-fluorophore complexes exhibit fluorescence that is more compatible with existing microscopy filter sets and allows Spinach2-tagged constructs to be imaged with either GFP or YFP filter cubes. Thus, these plug-and-play fluorophores allow the spectral properties of Spinach2 to be altered on the basis of the specific spectral needs of the experiment.
