124814-86-4Relevant academic research and scientific papers
Synthesis of Nonadeca-and Octadecaribonucleotides Using the Solid-Phase Phosphotriester with Tetrahydropyranyl Groups as the 2'-Hydroxyl-Protecting Group
Hirao, Ichiro,Ishikawa, Masahide,Hori, Hiroyuki,Watanabe, Kimitsuna,Miura, Kin-ichiro
, p. 1995 - 2001 (1989)
Nonadeca- and octadecaribonucleotides corresponding to the D-loop of tRNAPhe from yeast and the leader sequence of phage fl coat protein mRNA were synthesized by the activated phosphotriester method.Coupling yield in the synthesis of oligoribonucleotides depended on the extent of nucleosides loaded on controlled pore glass beads (CPG).N-Acyl-5'-O-dimethoxytrityl-2'-O-tetrahydropyranyl derivates were used as fully protected ribonucleotide monomer units.The 18mer and 19mer corresponding to the D-loop did not serve as substrates for tRNA (guanosine-2'-)methyltransferase from T. thermophilus, but inhibited methylation of the 5'-half fragment of tRNAPhe.This indicates that both fragments possess some affinity with the enzyme.
Synthesis of Oligoribonucleotides by the Hydroxybenzotriazole-Activated Phosphotriester/Dicyclohexylcarbodiimide System
Hirao, Ichiro,Miura, Kin-ichiro
, p. 1799 - 1802 (2007/10/02)
Addition of dicyclohexylcarbodiimide stabilized the benzotriazole-activated phosphotriester intermediates in which were used oligoribonucleotide synthesis.By this system, two oligoribonucleotides, r(CGAAAGC) and r(GCGAAAGC), were synthesized for structural comparison with d(GCGAAAGC) whose structure was unusually stable.Electrophoretic experiments showed r(GCGAAAGC) to behave differently from d(GCGAAAGC), although each had the same base sequence.
