Synthesis of Nonadeca-and Octadecaribonucleotides Using the Solid-Phase Phosphotriester with Tetrahydropyranyl Groups as the 2'-Hydroxyl-Protecting Group

Article abstract of DOI:10.1246/bcsj.62.1995

Nonadeca- and octadecaribonucleotides corresponding to the D-loop of tRNA^Phe from yeast and the leader sequence of phage fl coat protein mRNA were synthesized by the activated phosphotriester method.Coupling yield in the synthesis of oligoribonucleotides depended on the extent of nucleosides loaded on controlled pore glass beads (CPG).N-Acyl-5'-O-dimethoxytrityl-2'-O-tetrahydropyranyl derivates were used as fully protected ribonucleotide monomer units.The 18mer and 19mer corresponding to the D-loop did not serve as substrates for tRNA (guanosine-2'-)methyltransferase from T. thermophilus, but inhibited methylation of the 5'-half fragment of tRNA^Phe.This indicates that both fragments possess some affinity with the enzyme.