1384271-41-3Relevant academic research and scientific papers
Convenient Entry to 18F-Labeled Amines through the Staudinger Reduction
Stéen, E. Johanna L.,Shalgunov, Vladimir,Denk, Christoph,Mikula, Hannes,Kj?r, Andreas,Kristensen, Jesper L.,Herth, Matthias M.
, p. 1722 - 1725 (2019/01/30)
Fluorine-18 possesses outstanding decay characteristics for positron emission tomography (PET) imaging. Therefore, it is ideally suited for clinical applications. As such, improved strategies to incorporate fluorine-18 into bioactive molecules are of utmo
Two-color glycan labeling of live cells by a combination of diels-alder and click chemistry
Niederwieser, Andrea,Spaete, Anne-Katrin,Nguyen, Long Duc,Juengst, Christian,Reutter, Werner,Wittmann, Valentin
supporting information, p. 4265 - 4268 (2013/05/09)
One is not enough: Terminal alkenes are used as chemical reporters and ligation partners for 1,2,4,5-tetrazines in a Diels-Alder reaction with inverse electron demand (DARinv). Combination with strain-promoted azide-alkyne cycloaddition (SPAAC) allows the visualization of two different glycan structures in one experiment. Copyright
Preparation of carbohydrate arrays by using Diels-Alder reactions with inverse electron demand
Beckmann, Henning S. G.,Niederwieser, Andrea,Wiessler, Manfred,Wittmann, Valentin
, p. 6548 - 6554 (2012/06/30)
Carbohydrate microarrays are an emerging tool for the high-throughput screening of carbohydrate-protein interactions that represent the basis of many biologically and medicinally relevant processes. The crucial step in the preparation of carbohydrate arrays is the attachment of carbohydrate probes to the surface. We examined the Diels-Alder reaction with inverse-electron-demand (DARinv) as an irreversible, chemoselective ligation reaction for that purpose. After having shown the efficiency of the DARinv in solution, we prepared a series of carbohydrate-dienophile conjugates that were printed onto tetrazine-modified glass slides. Binding experiments with fluorescently labeled lectins proved successful and homogeneous immobilization was achieved by the DARinv. For immobilization of nonfunctionalized reducing oligosaccharides we developed a bifunctional chemoselective linker that enabled the attachment of a dienophile tag to the oligosaccharides through oxime ligation. The conjugates obtained were successfully immobilized on glass slides. The presented strategies for the immobilization of both synthetic carbohydrate derivatives and unprotected reducing oligosaccharides facilitate the preparation of high-quality carbohydrate microarrays by means of the chemoselective DARinv. This concept can be readily adapted for the preparation of other biomolecule arrays. Copyright
