138494-31-2

Upstream product

• 124482-92-4 2-cyanoethyl-N,N-(diisopropylamino)chlorophosphine 
• 138078-31-6 6-N-benzoyl-2'-O-(1-(2-chloroethoxy)ethyl)-5'-O-dimethoxytrityladenosine 
• 138078-24-7 N⁶-benzoyl-2'-O-<1-(2-chloroethoxy)ethyl>adenosine 
• 79154-57-7 3',5'-O-(tetraisopropyldisiloxane-1,3-diyl)-N₆-benzoyladenosine 
• 40615-36-9 4,4'-dimethoxytrityl chloride 

Relevant academic research and scientific papers

SOLID PHASE SYNTHESIS OF OLIGORIBONUCLEOTIDES BY THE PHOSPHORAMIDITE APPROACH USING 2'-O-1-(2-CHLOROETHOXY)ETHYL PROTECTION

The new type protecting group, 1-(2-chloroethoxy)ethyl (Cee) group has been employed for the protection of the 2'-OH groups of ribonucleoside residues in the synthesis of oligoribonucleotides by the phosphoramidite approach on a solid support, using the acid-labile 5'-O-dimethoxytrityl (DMTr) group.This group is completely stable under the acidic conditions required to remove the 5'-terminal protecting groups in oligonucleotide synthesis on a solid support, and yet is easily removable under mild condition of acidic hydrolysis (pH 2.0) for the final unblocking step.The Cee-protected ribonucleoside 3'-phosphoramidite units were evaluated in the synthesis of a series of oligoribonucleotides consisting of the homopolymers of cytidine, the box 9R and 9R' sequences of Tetrahymena rRNA, and a leader sequence of phage Qβ-A protein mRNA.A full data for the deprotection and purification of synthetic oligoribonucleotides are also described.