1398940-15-2Relevant academic research and scientific papers
A dual-fluorophore sensor approach for ratiometric fluorescence imaging of potassium in living cells
Wang, Zeming,Detomasi, Tyler C.,Chang, Christopher J.
, p. 1720 - 1729 (2021)
Potassium is the most abundant intracellular metal in the body, playing vital roles in regulating intracellular fluid volume, nutrient transport, and cell-to-cell communication through nerve and muscle contraction. On the other hand, aberrant alterations in K+homeostasis contribute to a diverse array of diseases spanning cardiovascular and neurological disorders to diabetes to kidney disease to cancer. There is an unmet need for studies of K+physiology and pathology owing to the large differences in intracellularversusextracellular K+concentrations ([K+]intra= 150 mM, [K+]extra= 3-5 mM). With a relative dearth of methods to reliably measure dynamic changes in intracellular K+in biological specimens that meet the dual challenges of low affinity and high selectivity for K+, particularly over Na+, currently available fluorescent K+sensors are largely optimized with high-affinity receptors that are more amenable for extracellular K+detection. We report the design, synthesis, and biological evaluation of Ratiometric Potassium Sensor 1 (RPS-1), a dual-fluorophore sensor that enables ratiometric fluorescence imaging of intracellular potassium in living systems.RPS-1links a potassium-responsive fluorescent sensor fragment (PS525) with a low-affinity, high-selectivity crown ether receptor for K+to a potassium-insensitive reference fluorophore (Coumarin 343) as an internal calibration standard through ester bonds. Upon intracellular delivery, esterase-directed cleavage splits these two dyes into separate fragments to enable ratiometric detection of K+.RPS-1responds to K+in aqueous buffer with high selectivity over competing metal ions and is sensitive to potassium ions at steady-state intracellular levels and can respond to decreases or increases from that basal set point. Moreover,RPS-1was applied for comparative screening of K+pools across a panel of different cancer cell lines, revealing elevations in basal intracellular K+in metastatic breast cancer cell linesvs.normal breast cells. This work provides a unique chemical tool for the study of intracellular potassium dynamics and a starting point for the design of other ratiometric fluorescent sensors based on two-fluorophore approaches that do not rely on FRET or related energy transfer designs.
Potassium ion fluorescent probe as well as preparation method and application thereof (by machine translation)
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, (2019/11/21)
The invention relates to a potassium ion fluorescent probe and a preparation method and application thereof (I). The potassium ion fluorescent probe provided by the invention NK1 has the advantages of short synthesis steps and simple structure, wherein, the synthesis steps of the potassium ion fluorescent probe are K ACLE as follows. + To the identification unit BODIPY, the derivative serves TPP as a fluorophore and as a mitochondria targeting group and is matched, so that the potassium ion fluorescent probe has high selectivity and sensitivity in the process of recognizing potassium ions. The NK1 human cervical cancer (HeLa) cell is small in toxic and side effects, high in biocompatibility and capable of targeting mitochondria. In addition, the method provided by the NK1 invention can qualitatively monitor the intracellular or outflow of potassium ions in the cell mitochondria. (by machine translation)
A highly K+-selective phenylaza-[18]crown-6-lariat-ether-based fluoroionophore and its application in the sensing of K+ ions with an optical sensor film and in cells
Ast, Sandra,Schwarze, Thomas,Mueller, Holger,Sukhanov, Aleksey,Michaelis, Stefanie,Wegener, Joachim,Wolfbeis, Otto S.,Koerzdoerfer, Thomas,Duerkop, Axel,Holdt, Hans-Juergen
, p. 14911 - 14917 (2013/11/06)
Herein, we report the synthesis of two phenylaza-[18]crown-6 lariat ethers with a coumarin fluorophore (1 and 2) and we reveal that compound 1 is an excellent probe for K+ ions under simulated physiological conditions. The presence of a 2-methoxyethoxy lariat group at the ortho position of the anilino moiety is crucial to the substantially increased stability of compounds 1 and 2 over their lariat-free phenylaza-[18]crown-6 ether analogues. Probe 1 shows a high K+/Na+ selectivity and a 2.5-fold fluorescence enhancement was observed in the presence of 100mM K+ ions. A fluorescent membrane sensor, which was prepared by incorporating probe 1 into a hydrogel, showed a fully reversible response, a response time of 150s, and a signal change of 7.8 % per 1mM K+ within the range 1-10mM K+. The membrane was easily fabricated (only a single sensing layer on a solid polyester support), yet no leaching was observed. Moreover, compound 1 rapidly permeated into cells, was cytocompatible, and was suitable for the fluorescent imaging of K+ ions on both the extracellular and intracellular levels. Copyright
Π (PI)-CONJUGATED FLUOROIONOPHORES AND METHOD FOR DETERMINING AN ALKALI ION
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Page/Page column 56-57, (2012/09/22)
The present invention relates to π-conjugated fluoroionophores, methods for their preparation, and their use and a method for determining an alkali ion. Fluoroionophoric compounds of the general formula I are described Ionophore – π-Linker - Fluorophore (
