1455429-77-2Relevant academic research and scientific papers
Solid phase synthesis and self-assembly of higher-order siRNAs and their bioconjugates
Cultrara, Christopher N.,Shah, Sunil,Kozuch, Stephen D.,Patel, Mayurbhai R.,Sabatino, David
, p. 999 - 1010 (2019)
New methods for the synthesis of higher-order siRNA motifs and their bioconjugates have recently gained widespread attention in the development of new and improved gene therapeutics. Our efforts aim to produce new chemical tools and protocols for the gene
Solid-phase synthesis, characterization and RNAi activity of branch and hyperbranch siRNAs
Maina, Anthony,Blackman, Brittany A.,Parronchi, Christopher J.,Morozko, Eva,Bender, Maria E.,Blake, Allan D.,Sabatino, David
, p. 5270 - 5274 (2013/09/23)
Linear, branch and hyperbranch siRNAs were effectively prepared for down-regulating GRP78 expression and inducing cell death in HepG2 liver cancer cells. Branch and hyperbranch GRP78 siRNAs were synthesized by automated solid-phase synthesis in good yields (44-78%) and isolated in excellent purities (>99%) following HPLC purification. Moreover, siRNAs adopted stable intramolecular hybrids as discerned by native PAGE and thermal denaturation studies. These sequences also exhibited the pre-requisite A-type helical trajectory for triggering RNAi activity as determined by CD spectroscopy. Biological studies confirmed potent suppression of GRP78 expression (50-60%) while compromising cancer cell viability by ～20%. Thus, branch and hyperbranch siRNAs may serve as potent siRNA candidates in cancer gene therapy applications.
