146368-13-0

Basic information

• Product Name: Cy3
• Synonyms: Sulfo Cy3 Carboxylic acids(ethyl);Sulfo-Cy3-acid;2-(3-(1-(5-Carboxypentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)prop-1-en-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate;3H-Indolium, 2-[3-[1-(5-carboxypentyl)-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-, inner salt
• CAS NO: 146368-13-0
• Molecular Formula: C₃₁H₃₈N₂O₈S₂
• Mol File: 146368-13-0.mol
• Article Data: 6

Chemical Properties

• Appearance: /
• Storage Temp.: under inert gas (nitrogen or Argon) at 2-8°C
• CAS DataBase Reference: Cy3(CAS DataBase Reference)
• NIST Chemistry Reference: Cy3(146368-13-0)
• EPA Substance Registry System: Cy3(146368-13-0)

Safety Data

• Hazardous Substances Data: 146368-13-0(Hazardous Substances Data)

Usage

Description

Sulfo-Cy3-acid is a fluorescent dye belonging to the cyanine dye family, known for its strong absorption and emission in the red spectral range. It is modified with sulfonate groups, enhancing its solubility in aqueous solutions, making it suitable for biological applications. Valued for its photostability and brightness, it is a crucial tool for fluorescent microscopy, flow cytometry, and other analytical techniques.

Uses

Used in Biological and Chemical Research:
Sulfo-Cy3-acid is used as a fluorescent label for biological and chemical research, enabling the visualization and tracking of biomolecules and chemical compounds due to its strong fluorescence properties in the red spectral range.
Used in Multi-color Labeling Experiments:
Sulfo-Cy3-acid is used as a component in multi-color labeling experiments for distinguishing and simultaneously visualizing multiple targets or markers within a sample, taking advantage of its compatibility with other fluorescence dyes.
Used in Fluorescent Microscopy:
Sulfo-Cy3-acid is used as a fluorescent probe in fluorescent microscopy for high-resolution imaging of cellular structures and processes, benefiting from its brightness and photostability.
Used in Flow Cytometry:
Sulfo-Cy3-acid is used as a fluorescent marker in flow cytometry for the rapid analysis and sorting of cells based on specific fluorescent signals, allowing for the assessment of cell characteristics and functions.
Used in Analytical Techniques:
Sulfo-Cy3-acid is used as a fluorescent indicator in various analytical techniques for the detection, quantification, and study of molecular interactions and processes, leveraging its strong fluorescence and aqueous solubility.

Upstream product

• 146368-08-3 1-(5-carboxypentyl)-2,3,3-trimethyl-3H-indolium-5-sulfonate 
• 122-51-0 orthoformic acid triethyl ester 
• 146368-07-2 1-Ethyl-2,3,3-Trimethylindolenineninium 5-Sulfone 
• 4224-70-8 6-bromohexanoic acid 
• 1251915-08-8 C₂₂H₂₄N₂O₄S 
• 146368-09-4 C₂₀H₂₂N₂O₃S 
• 132557-72-3 2,3,3-trimethylindole-5-sulfonic acid 
• 563-80-4 3-methyl-butan-2-one 
• 98-71-5 4-hydrazino-benzenesulfonic acid 
• 146368-09-4 (E)-1-ethyl-3,3-dimethyl-2-(2-(phenylamino)vinyl)-3H-indol-1-ium-5-sulfonate 

Downstream Products

• 1240125-45-4 Cy₃ N-hydroxysuccinimidyl ester 
• 146368-16-3 3H-indolium-1-(5-carboxypentyl)-2-[(1E,3E)-3-(1-ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl-5-sulfo-, inner salt, succinimidyl ester 

Relevant articles and documents

COMPOUNDS AND COMPOSITIONS FOR RETINAL INJURY DETECTION AND METHODS OF USING SAME

Described are compounds, compositions, and methods suitable for diagnosing individuals with eye injuries and/or diseases. The compounds of the present disclosure have fluorescent groups and bis-dipicolylamine groups, which may be substituted or unsubstituted. The fluorescent group and bis-dipicolylamine group are connected by linking groups. The compositions may be formulated and administered as an eye drop. The methods may be used to track and/or label dying cells associated with eye injuries and/or diseases, such as, for example, retinal degenerations including, but not limited to, retinitis pigmentosa, glaucoma, diabetic retinopathy, and age-related macular degeneration.

FLUORESCENCE COMPOUNDS AND PREPARATION METHOD THEREOF

Provided is a fluorescent compound represented by the following [Chemical Formula 1] and a method for preparing the same: wherein each of X, Y, R1, R2, R3 and n is the same as defined in the specification.

Synthesis of PEG derivatives bearing aminophenyl and their application for liquid-phase synthesis of water-soluble unsymmetrical cyanine dyes

The synthesis and NMR characterization of soluble PEG-supported polymers were described, and their subsequent application for liquid-phase synthesis of water-soluble cyanine dyes was also studied. Nucleophilic substitution of tosylation of PEG 16 with 1,3-bis(4-nitrophenoxy)-2-propanol 15 under basic conditions, followed by nitro group reduction, gave PEG-bound aminophenyl 18. Another PEG-bound aminophenyl 28 was prepared by condensation reaction of PEG-bound pentaerythritol 25 and 4-aminobenzoic acid followed by the cleavage of BOC group. Subsequent loading and activation of sulfoindoleninium to PEG derivatives 18 or 28 were achieved via simple strategies. Cyanine dyes were released by the attack of heterocyclic carbon nucleophile and the cleavage of PEG-bound hemicyanine without the chromatographic separation. The efficient, facile, and practical approaches appear to be robust and versatile strategies to deliver not only indocyanine dyes but also benzoindocyanine dyes.