172018-16-5Relevant articles and documents
Microbial transformation of 7-epi-10-deacetylbaccatin III to 10-deacetylbaccatin III
Feng, Xu,Sun, Lingzhi,Fu, Shaobing,Zou, Zhongmei,Sun, Di-An
, p. 45 - 47 (2010)
The microbial transformation of 7-epi-10-deacetylbaccatin III (7-epi-10-DAB III) to 10-deacetylbaccatin III (10-DAB III) was studied. In this report, seven microorganisms were found to be able to realize the transformation at yields from 20.0% to as high as 70.8%. The optimized conditions such as the solvent, pH value of the medium, the microorganisms, transformation time, and substrate concentration were investigated.
Microbial hydrolysis of 7-xylosyl-10-deacetyltaxol to 10-deacetyltaxol
Wang, Kang,Wang, Tingting,Li, Jianhua,Zou, Jianhua,Chen, Yongqin,Dai, Jungui
, p. 250 - 255 (2011)
Enterobacter sp. CGMCC 2487, a bacterial strain isolated from the soil around a Taxus cuspidata Sieb. et Zucc. plant, was able to remove the xylosyl group from 7-xylosyltaxanes. The xylosidase of this strain was an inducible enzyme. In the bioconversion of 7-xylosyl-10-deacetyltaxol (7-XDT) to 10-deacetyltaxol (10-DT), for the purpose of enhancing the conversion efficiency, the effects of NH4+, oat xylan, temperature, pH value, cell density and substrate concentration on the bioconversion have been systematically investigated. 3.0 mM NH4+, 0.6% oat xylan in the media could enhance the yield of 10-DT; the optimum biocatalytic temperature was 26 °C and optimum pH value was 6.0. The highest conversion rate and yield of 10-DT from 7-XDT reached 92% and 764 mg/L, respectively. In addition, the biocatalytic capacity of the cell cultures remained 66.1% after continuous three batches. These results indicate that converting 7-XDT to 10-DT, a useful intermediate for the semisynthesis of paclitaxel or other taxane-based anticancer drugs by a novel bacterial strain, Enterobacter sp. CGMCC 2487, would be an alternative for the practical application in the future.
PROCESS FOR THE PURIFICATION 10-DEACETYBACCATINE III FROM 10-DEACETYL-2-DEBENZOYL-2-PENTENOYLBACCATINE III
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Page/Page column 2, (2009/12/23)
A process for the preparation of 10-deacetyl-7,10-bis-trichloroacetylbaccatine III with HPLC purity higher than 99% and free from 2-debenzoyl-2-pentenoylbaccatine III by purification of 10-deacetyl-7,10-bis-trichloroacetylbaccatineIII followed by alkaline hydrolysis of the protecting groups in position 7 and 10.