1812-30-2 Usage
Description
Bromazepam is a psychoactive benzodiazepine derivative and prescription drug, commonly known by the brand name Lectopam. It is a crystalline solid that is often abused and associated with drug-facilitated crimes. This product is primarily intended for forensic and research applications.
Uses
Used in Pharmaceutical Industry:
Bromazepam is used as a controlled substance (depressant) for its anxiolytic properties, helping to alleviate anxiety and stress in patients.
Used in Forensic Applications:
Bromazepam is utilized in forensic science for the detection and analysis of its presence in cases related to drug-facilitated crimes, as it is a commonly abused substance in such scenarios.
Used in Research Applications:
As a benzodiazepine derivative, Bromazepam is employed in research settings to study its effects on the central nervous system, its potential for abuse, and the development of new treatments for anxiety and related disorders.
Originator
Lexotan,Roche,Italy,1975
Manufacturing Process
Example: 32.8 grams of 2-(2-aminobenzoyl)-pyridine and 200 cc of acetic
anhydride were stirred at room temperature for 3 hours and then permitted to
stand overnight. Evaporation to dryness and digestion of the residue with 200
cc of water containing a little sodium bicarbonate to make the pH slightly
alkaline gave 2-(2-acetamidobenzoyl)-pyridine as a light tan powder, which
upon crystallization from methanol formed colorless crystals melting at 151°-
153°C.
A solution of 8.6 cc of bromine in 100 cc of acetic acid was added slowly over
a 3.5 hour period to a stirred solution of 38.5 grams of 2-(2-
acetamidobenzoyl)-pyridinein 250 cc of acetic acid. The dark solution was
stirred for another 3 hours, permitted to stand over night, stirred for 1 hour
with N2 sweeping, and evaporated at diminished pressure in the hood. The
gummy residue (75 grams) was treated with water and ether, made alkaline
with dilute sodium bicarbonate solution, and separated. Both phases contained
undissolved product which was filtered off. Additional crops were obtained by
further extraction of the aqueous phase with ether and evaporation of the
resulting ether solutions. All these materials were recrystallized from methanol
(decolorizing carbon added) yielding 2-(2-acetamido-5-bromobenzoyl)-
pyridineas yellow crystals melting at 131.5°-133°C.
20.85 grams of 2-(2-acetamido-5-bromobenzoyl)-pyridinein 250 cc of 20%
hydrochloric acid in ethanol were heated to reflux for 2 hours. 100 cc of
alcohol were added after one hour to maintain fluidity. The mixture stood
overnight, was chilled and filtered to give 20.5 grams of colorless crystalline
2-(2-amino-5-bromobenzoyl)-pyridinehydrochloride. Digestion of this hydrochloride with 0.5liter hot water hydrolyzed this product to the free base,
2-(2-amino-5-bromobenzoyl)-pyridine which formed yellow crystals, melting
at 98°-100°C. Evaporation of the alcoholic mother liquor, water digestion of
the residue, and alkalization of the water digests afforded additional crops of
2-(2-amino-5-bromobenzoyl)pyridine.
0.145 kg of 2-(2-amino-5-bromobenzoyl)-pyridine, was dissolved in 2.0 liters
of glacial acetic acid. The resultant solution was placed in a 3 liter, 3-necked,
round bottom flask fitted with a stirrer, thermometer and dropping funnel. The
system was protected by a drying tube filled with anhydrous calcium chloride.
To the solution, with stirring at room temperature, were carefully added 46.7
ml of bromoacetyl bromide. After the addition was completed, the stirring was
continued for two hours. The mixture was then warmed to 40°C, stirred at
that temperature for 1.5 hours, chilled and filtered. The residue, after being
washed with glacial acetic acid, was dried in vacuo over flake potassium
hydroxide to give 2-(2-bromoacetamido-5-bromobenzoyl)-
pyridinehydrobromide orange crystals, MP 205°-206°C, dec.
The hydrobromide was hydrolyzed to the free base as follows: 0.119 kg of 2-
(2-bromoacetamido-5-bromobenzoyl)-pyridine hydrobromide was stirred with
1.2 liters of cold water for 3.5 hours. The mixture was chilled and filtered, and
the residue washed with cold water and dried to give 2-(2-bromoacetamido-5-
bromobenzoyl)-pyridine, MP 101°C (sinters), 103°-106°C, dec.
93.0 grams of 2-(2-bromoacetamido-5-bromobenzoyl)-pyridinewas carefully
added to 0.5 liter of anhydrous ammonia in a 1 liter, 3-necked, round bottom
flask equipped with stirrer and reflux condenser and cooled by a Dry Iceacetone
bath. The system was protected from moisture by a drying tube
containing anhydrous calcium chloride. After stirring for 2 hours, the cooling
bath was removed. The mixture was then stirred for 6 hours, during which
time the ammonia gradually boiled off. 0.4 liter of water was added to the
solid residue and stirrind was resumed for about 2 hours. The solid was then
filtered off, washed with water and dried in vacuo over potassium hydroxide
flakes. The residue was dissolved on a steam bath in 1.4 liters of ethyl
alcohol-acetonitrile (1:1) (decolorizing charcoal added). The solution was
filtered hot and the filtrate chilled overnight. The crystalline deposit was
filtered off, washed with cold ethyl alcohol and dried in vacuo over flake
potassium hydroxide to give 54.2 grams. 7-Bromo-1,3-dihydro-5-(2-pyridyl)-
2H-1,4-benzodiazepin-2-one, MP 238°C (sinters), 239°-240.5°, dec. Further
processing of the mother liquor yielded additional product.
Therapeutic Function
Tranquilizer
Enzyme inhibitor
This lipophilic valium-like benzodiazepine (FW = 316.20 g/mol; CAS 1812- 30-2; IUPAC Name: 7-bromo-5-(pyridin-2-yl)-1H-benzo[e][1,4]diazepin- 2(3H)-one), is a long-lasting anxiolytic agent used to treat anxiety or panic states. Like diazepam, bromazepam is a positive allosteric modulator of GABAA receptors, promoting GABA binding, which in turn increases the total conduction of chloride ions across the neuronal cell membrane. Like other benzodiazepines, bromazepam also inhibits the acetylcholine receptoroperated potassium current. Bromazepam is mainly metabolized by oxidative pathways within the liver.
Check Digit Verification of cas no
The CAS Registry Mumber 1812-30-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,8,1 and 2 respectively; the second part has 2 digits, 3 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 1812-30:
(6*1)+(5*8)+(4*1)+(3*2)+(2*3)+(1*0)=62
62 % 10 = 2
So 1812-30-2 is a valid CAS Registry Number.
InChI:InChI=1/C14H10BrN3O/c15-9-4-5-11-10(7-9)14(17-8-13(19)18-11)12-3-1-2-6-16-12/h1-7H,8H2,(H,18,19)
1812-30-2Relevant articles and documents
Study of acid hydrolysis of bromazepam
Damjanovi?, Tatjana,Popovi?, Gordana,Verbi?, Srdan,Pfendt, Lidija
, p. 1260 - 1265 (2004)
Quantitative study of an acid hydrolysis of bromazepam, which in acidic solution simultaneously undergoes reversible 4,5-azomethine bond cleavage and protolytic reactions, was performed (t = 25 °C). When an equilibrium is established, four species are sim
Improved synthesis of anxiolytic, anticonvulsant, and antinociceptive α2/α3-GABA(A)-ergic receptor subtype selective ligands as promising agents to treat anxiety, epilepsy, and neuropathic pain
Li, Guanguan,Golani, Lalit K.,Jahan, Rajwana,Rashid, Farjana,Cook, James M.
, p. 4124 - 4132 (2018/10/15)
An improved synthesis of the anxiolytic, anticonvulsant, and antinociceptive compounds: Hz-166, and its bioisosteres 1,2,4-oxadiazole (MP-III-080) and 1,3-oxazole (KRM-II-81) were synthesized in higher yields and with more facile purification methods (crystallization, etc.) in multigram quantities without column chromatography. In the synthesis of KRM-II-81, an alternative procedure was employed using the selective reducing reagent, potassium diisobutyl-tert-butoxyaluminum hydride (PDBBA), to prepare the desired C(3)-aldehyde in the absence of N(5)-C(6) imine reduction in good yield on a 20 gram scale.
New Synthesis of 7-Bromo-1,3-dihydro-3-hydroxy-5-(2'-pyridyl)-2H-1,4-benzoazepin-2-one
Kovac, Tomislav,Belin, Branka,Fajdiga, Tatjana,Sunjic, Vitomir
, p. 59 - 62 (2007/10/02)
A new synthesis of 7-bromo-1,3-dihydro-3-hydroxy-5-(2'-pyridyl)-2H-1,4-benzoazepin-2-one (5) is described.Starting from bromazepam (3), C(3) acylation with lead tetraacetate/potassium iodide in acetic acid affords 4, while its mild hydrolysis according ou