23491-45-4 Usage
Chemical Properties
yellow to yellow-green powder
Uses
Different sources of media describe the Uses of 23491-45-4 differently. You can refer to the following data:
1. Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence
2. Hoechst 33258 is a Hoechst stain used to stain DNA. It is part of a family of fluorescent dyes. Dyes and metabolites.
3. bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.Excitation max. =?346 nmEmission max. =?460 nm
General Description
Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.
Biochem/physiol Actions
bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.
Check Digit Verification of cas no
The CAS Registry Mumber 23491-45-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,3,4,9 and 1 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 23491-45:
(7*2)+(6*3)+(5*4)+(4*9)+(3*1)+(2*4)+(1*5)=104
104 % 10 = 4
So 23491-45-4 is a valid CAS Registry Number.
InChI:InChI=1/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
