2460-44-8Relevant articles and documents
Expression, purification and characterization of a bifunctional α-L-arabinofuranosidase/β-D-xylosidase from Trichoderma koningii G-39
Wan, Chin-Feng,Chen, Cheng-Ta,Huang, Lina,Li, Yaw-Kuen
, p. 109 - 116 (2007)
A gene of α-L-arabinofuranosidase (Abf) from Trichoderma koningii G-39 was successfully expressed in Pichia pastoris. The recombinant enzyme was purified to > 90% homogeneity by a cation-exchanged chromatography. The purified enzyme exhibits both α-L-arabinofuranosidase and β-D-xylosidase (Xyl) activities with p-nitrophenyl-α-L- arabionfuranoside (pNPAF) and 2,4-dinitrophenyl-β-D-xylopyanoside (2,4-DNPX) as substrate, respectively. The stability and the catalytic feature of the bifunctional enzyme were characterized. The enzyme was stable for at least 2 h at pH values between 2 and 8.3 at room temperature when assayed for Abf and Xyl activities. Enzyme activity decreased dramatically when the pH exceeded 9.5 or dropped below 1.5. The enzyme lost 35% of Abf activity after incubation at 55 °C for 2 h, but retained 95% of Xyl activity, with 2,4-DNXP as substrate, under the same conditions. Further investigation of the active site topology of both enzymatic functions was performed with the inhibition study of enzyme activities. The results revealed that methyl-α-L- arabinofuranoside inhibition is noncompetitive towards 2,4-DNPX as substrate but competitive towards pNPAF. Based on the thermal stability and the inhibition studies, we suggest that the enzymatic reactions of Abf and Xyl are performed at distinct catalytic sites. The recombinant enzyme possesses both the retaining transarabinofuranosyl and transxylopyranosyl activities, indicating both enzymatic reactions proceed through a two-step, double displacement mechanism.
Insight into Aluminum Sulfate-Catalyzed Xylan Conversion into Furfural in a Γ-Valerolactone/Water Biphasic Solvent under Microwave Conditions
Yang, Tao,Zhou, Yi-Han,Zhu, Sheng-Zhen,Pan, Hui,Huang, Yao-Bing
, p. 4066 - 4079 (2017)
A simple and efficient biphasic system with an earth-abundant metal salt catalyst was used to produce furfural from xylan with a high yield of up to 87.8 % under microwave conditions. Strikingly, the metal salt Al2(SO4)3 e
A solid-acid-based process for the conversion of hemicellulose
Dhepe, Paresh Laxmikant,Sahu, Ramakanta
, p. 2153 - 2156 (2010)
We describe a solid-acid-catalyzed conversion of hemicellulose (a component of lignocellulosic material) into xylose, arabinose and furfural in aqueous media.
New monoterpenoids from fruits of Gardenia jasminoides var. radicans
Yu, Shao-Fu,Huang, Xiao-Jun,Fu, Shu-Na,Wu, Chun,Ye, Wen-Cai,Zhou, Guang-Xiong
, p. 1267 - 1272 (2015)
One new lactone, cyclopentanepyrone A (1), and two new monoterpenoids, gardeterpenone A (2) and jasminoside V (3), were isolated from the fruits of Gardenia jasminoides var. radicans, along with four known monoterpenoids, 4-7, which were isolated from this plant for the first time. The structures of the isolates were elucidated by extensive spectroscopic studies, including UV, IR, 1D- and 2D-NMR, ESI-MS, HR-ESI-MS, and CD experiments.
Triterpenoid saponins from the roots of Clematis uncinata
Li, Shuo-Guo,Huang, Xiao-Jun,Li, Man-Mei,Wang, Mei,Feng, Rui-Bing,Zhang, Wei,Li, Yao-Lan,Wang, Ying,Ye, Wen-Cai
, p. 35 - 44 (2014)
Eight new bisdesmosidic triterpenoid saponins, clematiunicinosides A-H (1-8), along with eleven known ones (9-19), were isolated from the roots of Clematis uncinata. Their structures were elucidated on the basis of spectroscopic analysis and chemical evidence. All the isolated saponins were tested for their cytotoxic activities on human caski cervical cancer (Caski) cells, and compounds 13, 17 and 19 exhibited inhibitory effect on Caski cells.
Phenolic metabolites from the stems and leaves of sophora flavescens
Zhang, Chi,Wang, Yan-Min,Zhao, Feng-Chun,Chen, Liang-Mian,Zhang, Qi-Wei,Gao, Hui-Min,Wang, Zhi-Min
, p. 1516 - 1525 (2014)
Two new compounds, (-)-(6aR,11aR)-4-methoxy-8,9-(methylenedioxy)pterocarpan 3-O-β-D-glucopyranoside (1) and 5-hydroxy-7-methoxyisoflavone 4′-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside (2), were isolated, together with 30 known compounds from the stems and leaves of Sophora flavescens Aition. Their structures were elucidated by extensive spectroscopic analysis, including HRESI-MS data. A preliminary comparison of phenolic metabolite profiles, based on the qualitative HPLC analysis, indicated that the composition of the roots and the aerial parts were significantly different.
Stryphnosides G–P, 10 new triterpene glycosides from the pericarps of Stryphnodendron fissuratum
Yokosuka, Akihito,Okabe, Genki,Tatsuno, Satoru,Mimaki, Yoshihiro
, p. 18 - 26 (2016)
Ten new triterpene glycosides, stryphnosides G–P (1–10), were isolated from the pericarps of Stryphnodendron fissuratum (Legminosae). The structures of 1–10 were determined based on spectroscopic analyses, including various two-dimensional NMR spectroscopic techniques, and the results of hydrolytic cleavage. The sugar moiety attached to C-3 of the aglycone of 4, 5, and 7–10 is composed of five or six monosaccharides, of which the terminal α-L-arabinosyl unit has a1C4conformation. Compounds 2 and 5 differ from the other isolates in having an α-L-rhamnosyl unit at the C-21 hydroxy group. The cytotoxic activity of 1–10, stryphnosides A–F (11–16), and their aglycones (1a, 11a, 14a, and 16a) against HL-60?cells was also examined.
New triterpenoid saponin from the stems of Albizia adianthifolia (Schumach.) W.Wight
Toukea, Daniel Djou,Kamto, Eutrophe Le Doux,Simo, Line Made,Mbing, Joséphine Ngo,Antheaume, Cyril,Haddad, Mohamed,Noté, Olivier Placide,Pegnyemb, Dieudonné Emmanuel
, p. 780 - 788 (2020/08/19)
As part of our continuing study of apoptosis-inducing saponins from Cameroonian Albizia genus, one new triterpenoid saponin, named adianthifolioside J (1), together with the known gummiferaoside E (2), were isolated from Albizia adianthifolia stems. The s
Ursane-type triterpene oligoglycosides with anti-hepatosteatosis and anti-hyperlipidemic activity from the leaves of Ilex paraguariensis A. St.-Hil.
Inoue, Naoki,Konno, Takuya,Manse, Yoshiaki,Morikawa, Toshio,Muraoka, Osamu,Nagatomo, Akifumi,Ninomiya, Kiyofumi,Sakamoto, Chinatsu,Shibasaka, Aya,Sone, Mayuko,Xu, Yin,Yoshikawa, Masayuki
, (2022/03/19)
The methanol extract from the leaves of Ilex paraguariensis A. St.-Hil. (Aquifoliaceae), popularly known as mate, maté, or yerba maté, inhibits the intracellular triglyceride accumulation in HepG2 cells and suppresses the plasma triglyceride elevation in olive oil-treated mice. Three new triterpene saponins, termed mateosides I (1), II (2), and III (3), were isolated from the extract along with 29 known compounds. The structures of 1–3 were elucidated based on chemical and spectroscopic evidence. Among the isolates, principal saponin constituents, 2 and matesaponins 1 (7) and 2 (9), potently inhibited the triglyceride accumulation in HepG2 cells simultaneously treated with oleic acid and high glucose. In vivo assay of the methanol extract of I. paraguariensis revealed that 7 and 9 showed anti-hyperlipidemic activities in olive oil-treated mice. These results suggested that the saponin constituents of I. paraguariensis could be valuable bioactive marker for the anti-obesogenic activity. Graphical abstract: [Figure not available: see fulltext.]
Mimonoside D: a new triterpenoid saponin from Mimosa diplotricha Sauvalle (Fabaceae)
Dzoyem, Jean Paul,Kemkuignou, Blondelle Matio,Kenmogne, Claudie Fokou,Opatz, Till,Ponou, Beaudelaire Kemvoufo,Tchuenguem, Roland T.,Kühlborn, Jonas,Tapondjou, Léon Azefack,Teponno, Rémy Bertrand
, (2021/12/02)
A new triterpenoid saponin (Mimonoside D: 3-O-α-L-arabinopyranosyl-3β-hydroxyolean-12-en-28-oic acid 28-O-β-D-xylopyranosyl-(1→2)-β-D- glucopyranoside ester (1)) was isolated from the aerial parts of Mimosa diplotricha Sauvalle together with nine known compounds: 7,4′-dihydroxyflavone (2), kaempferol (3), lupeol (4), betulinic acid (5), β-sitosterol (6), β-sitosterol-3-O-β-D-glucopyranoside (7), lutein (8), 5,2′-dihydroxy-7,4′,5′-trimethoxyflavone (9) and vitexin (10). Their structures were elucidated on the basis of spectroscopic (1D and 2D nuclear magnetic resonance) and high-resolution mass spectrometric data as well as by comparison of their spectral data with those of related compounds. Compounds 2, 7 and 8 had already been isolated from M. diplotricha, while compounds 3, 4, 5 and 6 have been isolated from other Mimosa species. Compound 2 moderately inhibited Proteus mirabilis (MIC = 32μg/mL), weakly inhibited Pseudomonas aeruginosa (MIC = 64μg/mL) and very weakly inhibited Staphylococcus aureus (MIC = 128μg/mL) and Enterococus faecalis (MIC = 128μg/mL).
