3615-47-2Relevant academic research and scientific papers
A SPIROSTANOL GLYCOSIDE FROM AGAVE CANTALA
Sharma, S. C.,Sati, O. P.
, p. 1820 - 1821 (1982)
A new steroidal saponin has been isolated from the ethanolic extract of the roots of Agave cantala and shown to be 3-O--6-O--(25R)-5α-22α-O-spirostan-3β,6α-diol.Key Word Index - Agave cantala; Agavaceae; saponins; spirostanol glycoside; 3-O--6-O--(25R)-5α-22α-O-spirostan-3β,6α-diol.
A novel low-molecular-mass pumpkin polysaccharide: Structural characterization, antioxidant activity, and hypoglycemic potential
Huang, Linlin,Li, Fei,Li, Quanhong,Liang, Li,Wei, Yunlu,Yu, Guoyong
, (2020/10/02)
The novel natural low-molecular-mass polysaccharide (SLWPP-3) from pumpkin (Cucurbia moschata) was separated from the waste supernatant after macromolecular polysaccharide production and purified using a DEAE cellulose-52 column and gel-filtration chromatography. Chemical and instrumental studies revealed that SLWPP-3 with a molecular mass of 3.5 kDa was composed of rhamnose, glucose, arabinose, galactose and uronic acid with a weight ratio of 1: 1: 4: 6: 15, and primarily contained →3,6)-β-D-Galp-(1→, →4)-α-GalpA-(1→(OMe), →4)-α-GalpA-(1→, →2,4)-α-D-Rhap-(1→, →3)-β-D-Galp-(1→, →4)-α-D-Glcp, and →4)-β-D-Galp residues in the backbone. The branch chain passes were connected to the main chain through the O-4 atom of glucose and O-3 atom of arabinose. Physiologically, the ability of SLWPP-3 to inhibit carbohydrate-digesting enzymes and DPPH and ABTS radicals, as well as protect pancreatic β cells from oxidative damage by decreasing MDA levels and increasing SOD activities, was confirmed. The findings elucidated the structural types of pumpkin polysaccharides and revealed a potential adjuvant natural product with hypoglycemic effects.
Antibacterial Activity of a New Flavone Glycoside from the Stems of Holmskioldia sanguinea Retz.
Yadava,Raghuvansi, Jyotsna
, p. 1815 - 1818 (2019/07/18)
A new flavone glycoside was isolated from ethanolic extract of stem parts of Holmskioldia sanguinea Retz. Its structure was characterized as 3,4′-dihydroxy-5,7-dimethoxyflavone-3-O-β-D-galactopyranosyl(1→4)-O-β-D-arabinopyranosyl-4′-O-α-L-rhamnopyranoside (1) by colour reactions, chemical degradation and spectroscopic analysis. Antibacterial activity of compound 1 was evaluated against various Gram positive and Gram negative bacteria showing a significant effects.
Structural elucidation, antioxidant and immunomodulatory activities of a novel heteropolysaccharide from cultured Paecilomyces cicadae (Miquel.) Samson
Wang, Yanbin,He, Pengfei,He, Liang,Huang, Qingrong,Cheng, Junwen,Li, Weiqi,Liu, Yu,Wei, Chaoyang
, p. 270 - 281 (2019/04/17)
The fine structure and chain conformation of a heteropolysaccharide (PCIPS3) from mycelium of Paecilomyces cicadae were investigated via the analysis of HPLC, IR, methylation, NMR spectroscopy and multiangle light scattering. It was determined to be a 2.23 × 104 g/mol heteropolysaccharide primarily composed of glucose, galactose and mannose in a molar ratio of 23.8:2.1:1.0. The PCIPS3 backbone consisted of 1,4-linked α-D-Glcp and 1,4-linked 6-O-Me-α-D-Glcp residues, which were occasionally interrupted by branched β-Galf residues through 1,6-linkage. Moreover, the α (0.60) from Mark–Houwink–Sakurada (MHS) equation suggested that PCIPS3 adopted a flexible chain conformation in 0.1 mol/L NaNO3 at 25 °C. The worm-like chains model parameters for PCIPS3 were estimated as following: ML = 437 nm?1, q = 0.46 nm and 0.79 nm, which were further evidenced by AFM. Furthermore, PCIPS3 showed excellent scavenging capacities of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, ORAC radical and moderate immunomodulatory activity.
Isolation, purification, characterization and antioxidant activity of polysaccharides from the stem barks of Acanthopanax leucorrhizus
Hu, Hao-Bin,Liang, Hai-Peng,Li, Hai-Ming,Yuan, Run-Nan,Sun, Jiao,Zhang, La-La,Han, Ming-Hu,Wu, Yun
, p. 359 - 367 (2018/05/29)
A novel water-soluble polysaccharide (named ALP-1) was successfully isolated from the stem barks of Acanthopanax leucorrhizus by hot-water extraction, and further purified by Cellulose DEAE-52 and Sephadex G-100 chromatography. The structure of ALP-1 was characterized by HPLC, HPGPC, partial acid hydrolysis, periodate oxidation, Smith degradation, methylation, together with UV, IR and NMR spectral analysis. The antioxidant activities also were evaluated in vitro. Structural analysis revealed that ALP-1 was a homogeneous galactan with the average molecular weight of 169 kDa, composed of galactose, glucose, mannose and arabinose in a molar ratio of 6.1:2.1:1.1:1.0, owning a backbone structure of 1,6-linked α-D-Galp residues with some branches of α-D-Manp-(1 → 3)-α-L-Araf residues at O-3 and α-D-Galp residues at O-4 of 1,6-linked α-D-Galp. Antioxidant assay showed that ALP-1 exhibited strong DPPH[rad] and HO[rad] scavenging activities, as well as ferric-reducing antioxidant power. These results provide a scientific basis for the further use of polysaccharides from A. leucorrhizus.
Allelopathic effect of triterpenoid saponins from the stems of lathyrus aphaca linn
Yadava, Raj N.,Asati, Nidhi
, p. 177 - 184 (2018/09/14)
Two new triterpenoid saponins have been isolated from the methanolic extract of the stems of the Lathyrus aphaca Linn. A new compound 1 has m.p. 295?297°C, m.f. C47H78O16, [M]+ m/z 898 was characterized as 3-O-[β-D-galactopyranosyl-(14)]-β-D-xylopyranosyl-3β,11α,28-trihydroxy-olean-12-ene-28-O-α-L-rhamnopyranoside and compound 2 has m. p. 289-291°C, m.f. C46H76O16, [M]+ m/z 884 was characterized as 3-O-[β-D-xylopyranosyl-(1→4)]-β-D-glucopyranosyl-(1→4) α-L-arabinofuranosyl 2α,3β,19α-trihydroxy-olean-12-ene by various color reactions, chemical degradations, and spectral analysis. From experimental findings, it was concluded that both compounds 1 and 2 have shown allelopathic effects on the growth of shoot length of the seeds of Zea mays (L.).
Structural characterization and protective effect against murine sepsis of fucogalactans from Agaricus bisporus and Lactarius rufus
Ruthes, Andrea C.,Rattmann, Yanna D.,Carbonero, Elaine R.,Gorin, Philip A.J.,Iacomini, Marcello
experimental part, p. 1620 - 1627 (2012/04/11)
Fucogalactans from edible Agaricus bisporus (RFP-Ab) and wild Lactarius rufus (RFP-Lr) mushrooms were obtained on aqueous extraction followed by purification. RFP-Ab had Mw 43.8 × 104 g mol -1 and RFP-Lr Mw 1.4
Two antifungal active triterpenoid saponins from the seeds of Lathyrus plants
Khan, Noor Afshan
experimental part, p. 1687 - 1694 (2012/01/14)
Two novel triterpenoid glycosides have been isolated from butanolic seeds extract of two varieties of Lathyrus plants, i.e. Lathyrus ratan and Lathyrus aphaca. Their structures were elucidated as 3-O-[β-D-glucuronopyranosyl- (1→4)-α-L-arabinopyranosyl-(1→2)-α-L-arabinopyranosyl] -olean-11,13 (18)-dien-28-oic acid (1) and 3-O-{β-D-xylopyranosyl-(1 → 2)-β-D-glcopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]- α-D-xylopyranosyl}-2, 16-dihydroxy-4-hydroxymethyl urs-12-en-28-oic acid (2) on the basis of spectral evidences, i.e. FTIR, 1H-NMR, 13C-NMR, ESI-MS and FAB-MS data. The isolated saponins were tested for their antifungal activity. Compound 1 showed maximum inhibition against Colletotrichum dematium (77.8%), whereas compound 2 showed maximum inhibition against Alternaria alternata (53.9%).
Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synthesis
Kang, Hee Kyoung,Kimura, Atsuo,Kim, Doman
experimental part, p. 4148 - 4155 (2011/10/30)
The variations in glucosidic linkage specificity observed in products of different glucansucrases appear to be based on relatively small differences in amino acid sequences in their sugar-binding acceptor subsites. Various amino acid mutations near active sites of DSRBCB4 dextransucrase from Leuconostoc mesenteroides B-1299CB4 were constructed. A triple amino acid mutation (S642N/E643N/V644S) immediately next to the catalytic D641 (putative transition state stabilizing residue) converted DSRBCB4 enzyme from the synthesis of mainly α-(1→6) dextran to the synthesis of α-(1→6) glucan containing branches of α-(1→3) and α-(1→4) glucosidic linkages. The subsequent introduction of mutation V532P/V535I, located next to the catalytic D530 (nucleophile), resulted in the synthesis of an α-glucan containing increased branched α-(1→4) glucosidic linkages (approximately 11%). The results indicate that mutagenesis can guide glucansucrase toward the synthesis of various oligosaccharides or novel polysaccharides with completely altered linkages without compromising high transglycosylation activity and efficiency.
Glucomannans of serissa serissoides stem
Zhao, Hanmin,Zhou, Min,Huang, Meiyan,Wang, Kuiwu,Wei, Wanxing
experimental part, p. 176 - 178 (2011/09/19)
One β-D-glucomannan was isolated from Serissa serissoides (DC.) Druce. The average molecular weight of the polysaccharide was established to be 2.9 × 103 Da. The structure of the polysaccharide from S. serissoides (SSP) was investigated by using sugar and methylation analysis, infrared spectroscopy, mass spectrometry, and NMR spectroscopy. The experiments revealed that SSP consisted of β-D-Glcp and β-D-Manp units in the ratio of approximately 1.1:1 with β-D-(1→4)-Glcp and β-D-(1→2) Manp linkages
