458-35-5Relevant articles and documents
Two-Step One-Pot Synthesis of Pinoresinol from Eugenol in an Enzymatic Cascade
Ricklefs, Esther,Girhard, Marco,Koschorreck, Katja,Smit, Martha S.,Urlacher, Vlada B.
, p. 1857 - 1864 (2015)
The phytoestrogen pinoresinol is a high-value compound that has a protective effect against diverse health disorders, and thus is of interest for the pharmaceutical industry. Isolation of pinoresinol from plants suffers from low yields, and its chemical synthesis involves several work-up steps. In this study we devised a novel two-step one-pot enzymatic cascade combining a vanillyl-alcohol oxidase and a laccase for the production of pinoresinol from eugenol via the intermediate coniferyl alcohol. Along with the well-characterized vanillyl-alcohol oxidase from Penicillium simplicissimum used to catalyze the oxidation of eugenol, enzyme screening revealed three bacterial laccases that were appropriate for the synthesis of pinoresinol from coniferyl alcohol. The cascade was optimized regarding enzyme ratios, pH value, and the presence of organic solvents. Under optimized conditions, pinoresinol concentration achieved 4.4 mM (1.6 gl-1), and this compound was isolated and analyzed. Increasing value: The high-value compound pinoresinol is synthesized in a two-step one-pot process combining the vanillyl-alcohol oxidase from Penicillium simplicissimum (PsVAO) and a bacterial laccase starting from the inexpensive substrate eugenol. tBME=tert-butyl methyl ether.
On the role of the monolignol γ-carbon functionality in lignin biopolymerization
Holmgren, Anders,Norgren, Magnus,Zhang, Liming,Henriksson, Gunnar
, p. 147 - 155 (2009)
In order to investigate the importance of the monomeric γ-carbon chemistry in lignin biopolymerization and structure, synthetic lignins (dehydrogenation polymers; DHP) were made from monomers with different degrees of oxidation at the γ-carbon, i.e., carb
COMPOUNDS HAVING HEPATIAL DISEASE EFFECTIVE
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Paragraph 0114-0115; 0118, (2021/01/29)
The invention discloses a compound with a hepatopathy curative effect, and the compound is a compound shown as a general formula (I), an optical isomer or pharmaceutically acceptable salt thereof, canbe applied to treatment or prevention of hepatopathy, particularly to drugs for treating or preventing fatty liver, liver fibrosis or liver cirrhosis, and has a good application prospect.
Consolidated production of coniferol and other high-value aromatic alcohols directly from lignocellulosic biomass
Tramontina, Robson,Galman, James L.,Parmeggiani, Fabio,Derrington, Sasha R.,Bugg, Timothy D. H.,Turner, Nicholas J.,Squina, Fabio M.,Dixon, Neil
supporting information, p. 144 - 152 (2020/01/13)
Sustainable production of fine chemicals and biofuels from renewable biomass offers a potential alternative to the continued use of finite geological oil reserves. However, in order to compete with current petrochemical refinery processes, alternative biorefinery processes must overcome significant costs and productivity barriers. Herein, we demonstrate the biocatalytic production of the versatile chemical building block, coniferol, for the first time, directly from lignocellulosic biomass. Following the biocatalytic treatment of lignocellulose to release and convert ferulic acid with feruloyl esterase (XynZ), carboxylic acid reductase (CAR) and aldo-keto reductase (AKR), this whole cell catalytic cascade not only achieved equivalent release of ferulic acid from lignocellulose compared to alkaline hydrolysis, but also displayed efficient conversion of ferulic acid to coniferol. This system represents a consolidated biodegradation-biotransformation strategy for the production of high value fine chemicals from waste plant biomass, offering the potential to minimize environmental waste and add value to agro-industrial residues.
