5174-26-5Relevant academic research and scientific papers
α,β-Difluoromethylene deoxynucleoside 5′-triphosphates: A convenient synthesis of useful probes for DNA polymerase β structure and function
Upton, Thomas G.,Kashemirov, Boris A.,McKenna, Charles E.,Goodman, Myron F.,Prakash, G. K. Surya,Kultyshev, Roman,Batra, Vinod K.,Shock, David D.,Pedersen, Lars C.,Beard, William A.,Wilson, Samuel H.
, p. 1883 - 1886 (2009)
αβ-Difluoromethylene deoxynucleoside 5′-triphosphates (dNTPs, N = A or C) are advantageously obtained via phosphorylation of corresponding dNDP analogues using catalytic ATP, PEP, nucleoside diphosphate kinase, and pyruvate kinase. DNA pol β Kdd values for the α, β-CF2 and unmodified dNTPs, α, β-NH dUTP, and the α, β-CH2 analogues of dATP and dGTP are discussed in relation to the conformations of α, β-CF2 dTTP versus α, β-NH dUTP bound into the enzyme active site.2009 American Chemical Society.
Pyrimidine-purine and pyrimidine heterodinucleosides synthesis containing a triazole linkage
Lucas,Elchinger,Faugeras,Zerrouki
experimental part, p. 168 - 177 (2011/08/02)
This article describes a synthetic route to generate two purine-pyrimidine and pyrimidine heterodinucleosides. Both microwave activated regioselective alkylation using hydride and copper-catalyzed-azide-alkyne-cycloaddition (CuAAC) were used in order to perform the synthesis.
DNG cytidine: Synthesis and binding properties of octameric guanidinium-linked deoxycytidine oligomer
Szabo, Istvan E.,Bruice, Thomas C.
, p. 4233 - 4244 (2007/10/03)
The synthesis of guanidinium-linked cytidyl oligomer (DNG-C8), a cationic DNA analog, and the corresponding cytidine monomers is described. The DNG monomer synthesis was streamlined to produce a shorter route to the final monomer than previously reported for thymidine and subsequent solid-phase synthesis produced an octameric cytidyl DNG strand. Because octameric deoxyguanosine would be used as the complementary strand in our studies, it was necessary to investigate guanosine self-association. Singular value decomposition was used to mathematically deconvolve the spectral data and confirm the presence of transitions due to DNA-G8 self-association. Job plots show the binding stoichiometry of DNG-C8 with DNA-G 8 to be 1:1. Thermal denaturation studies of the DNG-C 8·DNA-G8 duplex established a T m≥90°C and a ΔG°=-13.3kcalmol-1, indicating the DNG-C8·DNA-G8 duplex is over 1000 times more stable than that of DNA-C8·DNA-G8.
