53-73-6 Usage
Originator
Hypertensin,Ciba,W. Germany,1961
Manufacturing Process
48 mg (0.042 mmol) of L-asparaginyl-L-arginyl-L-valyl-L-tyrosyl-L-valyl-Lhistidyl-
L-prolyl-L-phenylalanine methyl ester trihydrochloride are suspended
in 0.5 ml of methanol, and treated gradually in the course of one hour with
0.3 ml of N-caustic soda solution (about 7 equivalents) so that the pH value of
the solution is maintained between 10.5 and 11.5. After a further 30 minutes
the solution is freed from methanol under vacuum at room temperature,
adjusted with 1 N acetic acid to pH 7.4 and lyophilized. The residual mixture
of free peptide and inorganic salts (79 mg) is fractionated by countercurrent
distribution in the system butanol/0.1 N ammonium hydroxide. The pure
octapeptide is obtained as a colorless powder which is soluble in water and
methanol, more sparingly soluble in ethanol, and insoluble in acetone.
General Description
Angiotensin amide (Hypertensin)is a synthetic polypeptide (1-L-aspariginyl-5-L-valine angiotensinoctapeptide) and has twice the pressor activity ofangiotensin II. It is pharmaceutically available as alyophilized powder for injection (0.5–2.5 mg diluted in500 mL of sodium chloride injection or 5% dextrose for injection)to be administered by continuous infusion. The pressoreffect of angiotensin is due to an increase in peripheralresistance; it constricts resistance vessels but has little or nostimulating action on the heart and little effect on the capacitancevessels. Angiotensin has been used as an adjunct invarious hypotensive states. It is mainly useful in controllingacute hypotension during administration of general anestheticsthat sensitize the heart to the effects of catecholamines.
Check Digit Verification of cas no
The CAS Registry Mumber 53-73-6 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 5 and 3 respectively; the second part has 2 digits, 7 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 53-73:
(4*5)+(3*3)+(2*7)+(1*3)=46
46 % 10 = 6
So 53-73-6 is a valid CAS Registry Number.
InChI:InChI=1/C49H70N14O11/c1-26(2)39(61-42(67)33(12-8-18-55-49(52)53)57-41(66)32(50)23-38(51)65)45(70)58-34(20-29-14-16-31(64)17-15-29)43(68)62-40(27(3)4)46(71)59-35(22-30-24-54-25-56-30)47(72)63-19-9-13-37(63)44(69)60-36(48(73)74)21-28-10-6-5-7-11-28/h5-7,10-11,14-17,24-27,32-37,39-40,64H,8-9,12-13,18-23,50H2,1-4H3,(H2,51,65)(H,54,56)(H,57,66)(H,58,70)(H,59,71)(H,60,69)(H,61,67)(H,62,68)(H,73,74)(H4,52,53,55)/t32-,33-,34-,35-,36-,37-,39-,40-/m0/s1
53-73-6Relevant articles and documents
Interfacing droplet microfluidics with matrix-assisted laser desorption/ionization mass spectrometry: Label-free content analysis of single droplets
Kuester, Simon K.,Fagerer, Stephan R.,Verboket, Pascal E.,Eyer, Klaus,Jefimovs, Konstantins,Zenobi, Renato,Dittrich, Petra S.
, p. 1285 - 1289 (2013)
Droplet-based microfluidic systems have become a very powerful tool to miniaturize chemical and biological reactions. However, droplet content analysis remains challenging and relies almost exclusively on optical methods such as fluorescence spectroscopy. Hence, labeling of the analyte is typically required which impedes a more universal applicability of microdroplets. Here we present a novel interface coupling droplet microfluidics and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for label-free content analysis of single droplets. Nanoliter aqueous droplets immersed in perfluorinated oil are created in a microfluidic T-junction, transferred into a capillary, and deposited on a high-density microarray MALDI plate mounted on a motorized xy-stage. The fully automated system is robust and reliable due to two unique features. First, a simple optical droplet detection system is used to synchronize stage movement and exit of droplets from the capillary. Second, the microarray plate contains an array of over 26 000 hydrophilic spots within a hydrophobic coating, each spot acting as a recipient to confine the droplets and to prevent cross-contamination. The MALDI matrix can also be applied using our system by spotting matrix droplets on the microarray in a separate run. To demonstrate the potential of our system, we studied the enzymatic cleavage of angiotensin I by angiotensin converting enzyme and monitored the increasing concentration of the product angiotensin II over time. The interface provides a robust and fully automated method for rapid label-free and information-rich content analysis of single droplets. With the high number of droplets per plate, this method is particularly suitable for high-throughput screening applications.
INHIBITORY OCTAPEPTIDES ANGIOTENSIN II
-
, (2008/06/13)
Octapeptide derivatives characterized by an oxygen or sulfur-containing moiety in the C-terminal position are potent inhibitors of the pharmacological effects of Angiotensin and possess the additional advantage of a favorable antagonist/agonist ratio.
