60129-38-6

Usage

Uses

Used in Biochemistry and Molecular Biology:
DTNB-NHS is used as a linker for the attachment of proteins to gold nanoparticles in Surface-Enhanced Raman Scattering (SERS) based immunoassays. This allows for the detection and analysis of specific biomarkers, making it a valuable tool in the study of various biological processes and diseases.
Used in Medical Diagnostics:
DTNB-NHS is used as a probe in the detection of tuberculosis antigenic markers. Its ability to bind specifically to these markers allows for the accurate identification of tuberculosis, aiding in the diagnosis and treatment of the disease.

Upstream product

• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 69-78-3 5,5'-dithiobis-(2-nitrobenzoic acid) 

Downstream Products

• 250737-32-7 5-{3-[5-((3R,4R,5R,6R)-4,5-Dihydroxy-6-hydroxymethyl-2-oxo-piperidin-3-ylcarbamoyl)-pentylcarbamoyl]-4-nitro-phenyldisulfanyl}-2-nitro-benzoic acid 2,5-dioxo-pyrrolidin-1-yl ester 

Relevant academic research and scientific papers

Femtomolar Detection of Prostate-Specific Antigen: An Immunoassay Based on Surface-Enhanced Raman Scattering and Immunogold Labels

A novel reagent for low-level detection in immunoadsorbent assays is described. The reagent consists of gold nanoparticles modified to integrate bioselective species (e.g., antibodies) with molecular labels for the generation of intense, biolyte-selective

5,5 '-di-thio-( amber acyl imino-2-nitro-benzoic acid ester) method for the preparation of compounds

A 5,5 '-di-thio-( amber acyl imino-2-nitro-benzoic acid ester) method for the preparation of compounds (DSNB), comprising the following steps : (1) preparing a 5,5' -di-thio-(2-nitrobenzoic acid), bicyclic hexanoic carbodiimide, N-hydroxy amber acyl imine

Novel solid-phase reagents for facile formation of intramolecular disulfide bridges in peptides under mild conditions

The controlled formation of intramolecular disulfide bridges in peptides, while avoiding unwanted oligomerization, is a significant challenge. Ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid), was developed originally in the context of an assay for measuring free thiol concentration under physiological conditions. The present studies demonstrate that this reagent, when bound through two sites to a suitable solid support (PEG-PS, modified Sephadex, or controlled-pore glass), is an effective mild oxidizing reagent that promotes the formation of disulfide bridges. Rates and yields of the reactions were determined as a function of pH, excess of oxidizing reagent, resin loading, and parent support, for the preparation of oxytocin and deaminooxytocin (9 residues, disulfide bridge between residues 1 and 6), somatostatin (14 residues, disulfide bridge between residues 3 and 14), α-conotoxin SI (13 residues, disulfide bridges between residues 2 and 7; 3 and 13), and apamin (18 residues, disulfide bridges between residues 1 and 11; 3 and 15). Cystine dimers of these peptide models formed, if at all, in relatively low amounts. Use of solid-phase Ellman's reagents to oxidize the linear precursors of conotoxin or apamin (tetrathiols) gave as the main products the correctly paired regioisomers. Particular advantages of the overall approach include fast reaction rates over a wide range of pH, from 2.7 to 6.6; easy purification of disulfide-containing products; and the specificity and reusability of the reagents.