60686-82-0Relevant articles and documents
On the bi-enzymatic behaviour of Saccharomyces cerevisiae-mediated stereoselective biotransformation of 2,6,6-trimethylcyclohex-2-ene-1,4-dione
Uzir, Mohamad Hekarl,Najimudin, Nazalan
, p. 56 - 64 (2018/02/13)
Baker's yeast has been well-known to have the ability to reduce a variety of substrates into many optically active compounds. One of the important chemicals is (4R,6R)-4-hydroxy-2,2,6-trimethylcyclohexanone or in short, (4R,6R)-actinol, a product formed f
Synthesis of biotinylated retinoids for cross-linking and isolation of retinol binding proteins
Nesnas, Nasri,Rando, Robert R,Nakanishi, Koji
, p. 6577 - 6584 (2007/10/03)
The synthesis of (3R)-3-[Boc-Lys(biotinyl)-O]-11-cis-retinol bromoacetate and 3-[Boc-Lys(biotinyl)-O]-all trans-retinol chloroacetate is described. These biotinylated retinoids are instrumental in labeling the retinol binding proteins (RBPs) via a nucleophilic displacement of the haloacetate by a residue in the binding site of the protein. The covalently linked biotin will allow for a facile isolation and purification of the protein on a streptavidin column thus rendering the protein ready for a tryptic digest followed by mass spectrometric analysis. The 11-cis retinoid was synthesized via metal reduction of an alkyne intermediate generated from a Horner-Wadsworth-Emmons (HWE) reaction whereas the all-trans was synthesized via two consecutive HWE couplings.
Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding monovalent cation-activated levodione reductase from Corynebacterium aquaticum M-13.
Yoshisumi,Wada,Takagi,Shimizu,Nakamori
, p. 830 - 836 (2007/10/03)
The gene encoding (6R)-2,2,6-trimethyl-1,4-cyclohexanedione (levodione) reductase was cloned from the genomic DNA of the soil isolate bacterium Corynebacterium aquaticum M-13. The gene contained an open reading frame consisting of 801 nucleotides correspo