653-21-4Relevant articles and documents
On the hydrolysis of diethyl 2-(perfluorophenyl)malonate
Kiskin, Mikhail A.,Taydakov, Ilya V.
supporting information, p. 1863 - 1868 (2020/09/03)
Diethyl 2-(perfluorophenyl)malonate was synthesized in 47% isolated yield by the reaction of sodium diethyl malonate and hexafluorobenzene. The resulting compound was considered as a starting material for synthesizing 2-(perfluorophenyl)malonic acid by hydrolysis. It was found that the desired 2-(perfluorophenyl)malonic acid could not be obtained from this ester by hydrolysis, neither under basic nor under acidic conditions. Nevertheless, hydrolysis of the ester with a mixture of HBr and AcOH gave 2-(perfluorophenyl)acetic acid in a good preparative yield of 63%. A significant advantage of this new approach to 2-(perfluoro-phenyl)acetic acid is that handling toxic substances such as cyanides and perfluorinated benzyl halides is avoided.
Mechanism and Mechanism-Based Inactivation of 4-Hydroxyphenylpyruvate Dioxygenase
Forbes, Brian J.R.,Hamilton, Gordon A.
, p. 343 - 361 (2007/10/02)
Six substrate analogs of 4-hydroxyphenylpyruvate, specifically pentafluorophenylpyruvate, 4-hydroxytetrafluorophenylpyruvate, 2-thienylpyruvate, 3-thienylpyruvate, thiophenol oxalate, and p-thiocresol oxalate were synthesized and their interactions with porcine liver 4-hydroxyphenylpyruvate dioxygenase investigated.Both pentafluorophenylpyruvate and thiophenol oxalate are competitive inhibitors of the enzyme with K1 values of 14 and 150 μM, respectively, but p-thiocresol oxalate has no effect on the enzymic activity.The other three substrate analogs are both substrates and mechanism-based inactivators of the enzyme with the following kinetic characteristics (compound, Km, Vmax,kinact, K', partition ratio) at pH 6.0, 37 deg C, and an air atmosphere: 4-hydroxytetrafluorophenylpyruvate, 50 μM, 1.9 mkat/kg, 1.5/min, 70 μM, 4.2; 2-thienylpyruvate, 500μM, 7.8 mkat/kg, 0.6/min, 400 μM, 41; 3-thienylpyruvate, 250 μM, 2.9 mkat/kg, 0.6/min, 300 μM, 22.When inactivated, the dioxygenase was found to contain per mole of active enzyme, 0.78 mol of label from 3-thienyl-3pyruvate and 0.85 mol of label from 4-hydroxytetrafluorophenyl-3pyruvate.The product formed from the enzyme-catalyzed oxidation of 3-thienylpyruvate was determined to be 3-carboxymethyl-3-thiolene-2-one.The implication of these results to the mechanism of the dioxygenase is considered.
