664985-43-7

Usage

Uses

Used in Medicinal Chemistry and Drug Development:
4-[2-(DIMETHYLAMINO)ETHYLAMINOSULFONYL]-7-CHLORO-2,1,3-BENZOXADIAZOLE is used as a building block in the synthesis of pharmaceutical compounds, leveraging its unique structure to contribute to the development of new drugs.
Used in Biochemical and Cellular Assays:
4-[2-(DIMETHYLAMINO)ETHYLAMINOSULFONYL]-7-CHLORO-2,1,3-BENZOXADIAZOLE serves as a probe in biochemical and cellular assays, potentially aiding in the study of biological processes and the discovery of new therapeutic targets.
Used in Fluorescent Labeling and Imaging Studies:
Due to its chemical properties, 4-[2-(DIMETHYLAMINO)ETHYLAMINOSULFONYL]-7-CHLORO-2,1,3-BENZOXADIAZOLE may be utilized in fluorescent labeling and imaging studies, which can enhance the visualization and analysis of biological samples.
Further research and testing are necessary to fully explore the range of properties and potential applications of 4-[2-(DIMETHYLAMINO)ETHYLAMINOSULFONYL]-7-CHLORO-2,1,3-BENZOXADIAZOLE in various industries.

InChI:InChI=1/C10H13ClN4O3S/c1-15(2)6-5-12-19(16,17)8-4-3-7(11)9-10(8)14-18-13-9/h3-4,12H,5-6H2,1-2H3

Upstream product

• 142246-48-8 7-chlorobenzo[c][1,2,5]oxadiazole-4-sulfonic acid chloride 
• 108-00-9 N,N-dimethylethylenediamine 

Relevant academic research and scientific papers

Fluorogenic Derivatization Reagents Suitable for Isolation and Identification of Cysteine-Containing Proteins Utilizing High-Performance Liquid Chromatography - Tandem Mass Spectrometry

The fluorogenic derivatization reagents with a positive charge, 4-(dimethylaminoethylaminosulfonyl)-7-chloro-2,1,3-benzoxadiazole (DAABD-Cl) and 7-chloro-2,1,3-benzoxadiazole-4-sulfonylaminoethyltrimethylammonium chloride (TAABD-Cl), are proposed for use in proteomics studies. Following derivatization of protein mixtures with these reagents, a series of standard processes of isolation, digestion, and identification of the proteins were performed utilizing high-performance liquid chromatography-fluorescence detection and tandem mass spectrometry with the probability-based protein identification algorithm. Both DAABD and TAABD derivatives were detected fluorometrically at the femtomole level and showed more than 100-fold improvement in sensitivity compared to the underivatized original compounds with an electrospray ionization ion trap mass spectrometer analysis. The modification of the MASCOT database search system memorized with the fragment information of a DAABD-attached Cys residue allowed the identification of the proteolytic peptide fragments of the derivatized bovine serum albumin (BSA) with an estimated 38% sequence coverage of BSA. Utilizing DAABD-Cl as a derivatization reagent, identification of several proteins was also possible in a soluble extract of Caenorhabditis elegans (10 μg of protein). Consequently, for identification of proteins in the complex matrixes of proteins, DAABD-Cl could be a more appropriate reagent than ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate as reported previously.

Analytical method and apparatus using fingerprints on the basis of types in expression levels of express trace proteins and/or peptides contained in living tissue and/or biological fluid

Provided are an analytical method and apparatus using fingerprints on the basis of the types and expression levels of expressed trace proteins and/or peptides contained in living tissue and/or biological fluid. The present invention relates to a method and apparatus for analyzing the status of living tissue and/or biological fluid by binding a specific fluorogenic reagent (such as DAABD-Cl) to expressed trace proteins and/or peptides contained in living tissue and/or biological fluid without degradation treatment followed by precisely detecting and separating by nano-liquid chromatography, and subsequently continuously and quantitatively measuring the separated and purified fluorescent labelled proteins and/or peptides using a fluorescence detector. The use of a nano-liquid chromatograph having a fluorescence detector makes it possible to obtain profiles of expressed trace proteins and/or peptides contained in living tissue and/or biological fluid.